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Akademický článek

De Novo Transcriptome Analysis by PacBio SMRT-Seq and Illumina RNA-Seq Provides New Insights into Polyphenol Biosynthesis in Chinese Olive Fruit

Autor: Qinghua Ye, Shiyan Zhang, Qian Xie, Wei Wang, Zhehui Lin, Huiquan Wang, Yafang Yuan, Qingxi Chen

Publikováno v: Horticulturae, Vol 10, Iss 3, p 293 (2024)

Polyphenols play a crucial role in fruit flavor. To elucidate the mechanism of fruit polyphenol metabolism, we constructed a transcriptome atlas through PacBio single-molecule real-time (SMRT) sequencing and Illumina next-generation sequencing (NGS)

Externí odkaz: https://doaj.org/article/64ee7d54bfdc49d68a5361613ed5dcea

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Akademický článek

The transcription factor PbrbZIP52 positively affects pear pollen tube longevity by promoting callose synthesis.

Autor: Zhongheng Xia, Binxu Wen, Jing Shao, Tianci Zhang, Mengmeng Hu, Lin Lin, Yiping Zheng, Zhixin Shi, Xinlin Dong, Juanjuan Song, Yuanshan Li, Yongjie Wu, Yafang Yuan, Juyou Wu, Qingxi Chen, Jianqing Chen

Publikováno v: Plant Physiology; Mar2023, Vol. 191 Issue 3, p1734-1750, 17p

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The transcription factor PbrbZIP52 positively affects pear pollen tube longevity by promoting callose synthesis

Publikováno v: Plant physiology.

In pear (Pyrus bretschneideri), pollen tube growth is critical for the double fertilization associated with seed setting, which in turn affects fruit yield. The normal deposition of callose mediates the polar growth of pollen tubes. However, the mech

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c60ede647f3b21a8bd86201353bc203b
https://pubmed.ncbi.nlm.nih.gov/36617219

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Genome-wide identification and validation of optimal reference genes for gene expression normalization in pear peel

Autor: Changmei Chen, Xiayu Guan, Chen Zhiyi, Tengyun Wang, Zhenmei Cai, Yafang Yuan, Wang Wei, Guifeng Xie, Jianqing Chen, Qingxi Chen, Xie Qian

Publikováno v: Plant Growth Regulation. 92:249-261

Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) is the preferred method for gene expression research, but normalization based on suitable reference genes (RGs) is the key to obtaining reliable gene expression results. In this s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::1a5b80bd3f96ec9a8a109a55322f49f4
https://doi.org/10.1007/s10725-020-00634-z

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Genome-wide identification and testing of superior reference genes for transcript normalization during analyses of flesh development in Asian pear cultivars

Autor: Xie Qian, Xia Zhongheng, Xiayu Guan, Yafang Yuan, Meng Yuan, Changmei Chen, Jianqing Chen, Liu Yueying, Wang Wei, Juanjuan Song, Qingxi Chen

Publikováno v: Scientia Horticulturae. 271:109459

Quantitative real-time polymerase chain reaction (qRT-PCR) is currently the best available option for analyzing gene expression and transcriptomes. In this context, it is important to use ideal reference genes (RGs) to normalize the expression data f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::63badb4f9ff612965a2d9cdd3e2b7bd9
https://doi.org/10.1016/j.scienta.2020.109459

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