Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Yae Kurosawa"'
Publikováno v:
PLoS ONE, Vol 14, Iss 9, p e0222199 (2019)
Infectious virus purification techniques are important for vaccine development and gene therapy applications. However, the standardized one-step purification technique using ceramic hydroxyapatite (CHAp) has proven unsuitable for poliovirus. Therefor
Externí odkaz:
https://doaj.org/article/c678c725bb00461697aa51c32c00a9fc
Publikováno v:
PLoS ONE, Vol 8, Iss 1, p e53893 (2013)
Although ceramic hydroxyapatite (HAp) chromatography has been used as an alternative method ultracentrifugation for the production of vaccines, the mechanism of virus separation is still obscure. In order to begin to understand the mechanisms of viru
Externí odkaz:
https://doaj.org/article/6b3066d8c3694d5baa8979e1f5c840c4
Autor:
Enoch Y. Park, Shintaro Kobayashi, Robert Minkner, Rina Baba, Yae Kurosawa, Tatsuya Kato, Shinichiro Suzuki
Publikováno v:
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1096
A three-stage chromatography protocol for the purification of human papillomavirus-like particles (HPV-LPs) from the silkworm-based Bombyx mori nucleopolyhedrovirus bacmid expression system was developed. For host cell DNA separation, anion exchange
Publikováno v:
PLoS ONE
PLoS ONE, Vol 14, Iss 9, p e0222199 (2019)
PLoS ONE, Vol 14, Iss 9, p e0222199 (2019)
Infectious virus purification techniques are important for vaccine development and gene therapy applications. However, the standardized one-step purification technique using ceramic hydroxyapatite (CHAp) has proven unsuitable for poliovirus. Therefor
Autor:
Yee Yee Lwin, Yae Kurosawa, Tin Myint, Kay Thi Aye, Shingo Inoue, Kyaw Zin Thant, Pe Thet Khin, Khin Htwe, Filipinas F. Natividad, Mya Myat Ngwe Tun, Sanda Lin, Cynthia A. Mapua, Kenji Hirayama, Kouichi Morita
Publikováno v:
Journal of Medical Virology. 85:1258-1266
In Myanmar, dengue fever (DF)/dengue hemorrhagic fever (DHF) is one of the leading causes of morbidity and mortality among children. From Pyinmana Hospital in 2004 and Mandalay Children Hospital in 2006, 160 patients diagnosed clinically to have DHF/
Publikováno v:
Hybridoma. 31:68-71
Antibody purification using proteins A and G has been a standard method for research and industrial processes. The conventional method, however, includes a three-step process, including buffer exchange, before chromatography. In addition, proteins A
Publikováno v:
World Journal of Vaccines. :155-160
Dengue virus type 2 ThNH7/93 retained infectious activity after purification by ceramic hydroxyapatite chromatogra-phy. Dengue virus type 2 culture fluid was loaded onto the ceramic hydroxyapatite column and eluted with a linear gradient of sodium ph
Autor:
Ken Sugo, Shintaro Kobayashi, Katsumi Kawamura, Masahiro Tomita, Yae Kurosawa, Tsuneo Okuyama, Tomohiko Yoshitake
Publikováno v:
Separation and Purification Technology. 76:432-435
Histidine-tagged proteins have been purified with immobilized nickel affinity chromatography. This method, however, bears some serious disadvantages, including carcinogenicity and potential leakage of the nickel and the handling and disposal costs. W
Autor:
Cynthia A. Mapua, Kouichi Morita, Maria Theresa P. Alera, Kazunori Oishi, Maria T.G. Alonzo, Shingo Inoue, Ronald R. Matias, Filipinas F. Natividad, Yae Kurosawa, Efren M. Dimaano, Futoshi Hasebe, Joyce D. Reyes, Mariko Saito
Publikováno v:
Vector-Borne and Zoonotic Diseases. 10:143-150
To establish a new method for the diagnosis of dengue secondary infection, 187 serum samples from the patients with dengue secondary infection, 40 serum samples from the patients with dengue primary infection, and 44 serum samples from the healthy vo
Autor:
Robert G. Ridley, Raffaello Masciadri, Werner Hofheinz, Hisao Shimada, Hugues Matile, Tomoko Satoh, Arnulf Dorn, Michiko Kitsuji-Shirane, Manfred Kansy, Yae Kurosawa
Publikováno v:
Antimicrobial Agents and Chemotherapy. 44:2638-2644
Hematin polymerization is a parasite-specific process that enables the detoxification of heme following its release in the lysosomal digestive vacuole during hemoglobin degradation, and represents both an essential and a unique pharmacological drug t