Výsledky vyhledávání - "Yacov Ashani"

Utilization of diverse organophosphorus pollutants by marine bacteria

Autor: Dragana Despotović, Einav Aharon, Olena Trofimyuk, Artem Dubovetskyi, Kesava Phaneendra Cherukuri, Yacov Ashani, Or Eliason, Martin Sperfeld, Haim Leader, Andrea Castelli, Laura Fumagalli, Alon Savidor, Yishai Levin, Liam M. Longo, Einat Segev, Dan S. Tawfik

Publikováno v: Proceedings of the National Academy of Sciences. 119

Anthropogenic organophosphorus compounds (AOPCs), such as phosphotriesters, are used extensively as plasticizers, flame retardants, nerve agents, and pesticides. To date, only a handful of soil bacteria bearing a phosphotriesterase (PTE), the key enz

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8dea64629062f425106cb4a391722fe3
https://doi.org/10.1073/pnas.2203604119

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Torpedo californica acetylcholinesterase is stabilized by binding of a divalent metal ion to a novel and versatile 4D motif

Autor: Lev Weiner, Esther Roth, Israel Silman, Joel L. Sussman, Anne Nicolas, Valery L. Shnyrov, Yacov Ashani

Publikováno v: Protein Science : A Publication of the Protein Society

Stabilization of Torpedo californica acetylcholinesterase by the divalent cations Ca+2, Mg+2, and Mn+2 was investigated. All three substantially protect the enzyme from thermal inactivation. Electron paramagnetic resonance revealed one high‐affinit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b6efd6e2799b74058fdfb11797f65ca
https://doi.org/10.1002/pro.4061

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Quinone Methide‐Based Organophosphate Hydrolases Inhibitors: Trans Proximity Labelers versus Cis Labeling Activity‐Based Probes

Autor: Dan S. Tawfik, Laura Fumagalli, Gili Ben-Nissan, Anna Meshcheriakova, Artem Dubovetskyi, Eitan Reuveny, Kesava Phaneendra Cherukuri, Michal Sharon, Yacov Ashani

Publikováno v: ChemBioChem. 22:894-903

Quinone methide (QM) chemistry is widely applied including in enzyme inhibitors. Typically, enzyme-mediated bond breaking releases a phenol product that rearranges into an electrophilic QM that in turn covalently modifies protein side chains. However

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c1ffe650ecee4d3b4689c5f51a0d800
https://doi.org/10.1002/cbic.202000611

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Widespread Utilization of Diverse Organophosphate Pollutants by Marine Bacteria

Autor: Alon Savidor, Olena Trofimyuk, Andrea Castelli, Dan S. Tawfik, Yishai Levin, Artem Dubovetskyi, Liam M. Longo, Yacov Ashani, Dragana Despotovic, Einat Segev, Einav Aharon, Laura Fumagalli, Haim Leader, Kesava Phaneendra Cherukuri

Anthropogenic organophosphates (AOPs), such as phosphotriesters, are used extensively as plasticizers, flame retardants, nerve agents and pesticides. Soil bacteria bearing a phosphotriesterase (PTE) can degrade AOPs, but whether bacteria are capable

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::34856e5058887262ab012f3b884e6608
https://doi.org/10.1101/2021.10.18.464763

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Catalytic bioscavengers as countermeasures against organophosphate nerve agents

Autor: Yacov Ashani, Moshe Goldsmith

Publikováno v: Chemico-Biological Interactions. 292:50-64

Recent years have seen an increasing number of incidence, in which organophosphate nerve agents (OPNAs) have been used against civilians with devastating outcomes. Current medical countermeasures against OPNA intoxications are aimed at mitigating the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6bfdaaf3e3955029a1bb166087fb1725
https://doi.org/10.1016/j.cbi.2018.07.006

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Overcoming an optimization plateau in the directed evolution of highly efficient nerve agent bioscavengers

Autor: Per Jr Greisen, Sergey Ovchinnikov, Yacov Ashani, Sarel J. Fleishman, Joel L. Sussman, Moshe Goldsmith, Dan S. Tawfik, Halim Jubran, David Baker, Adi Goldenzweig, Nidhi Aggarwal, Haim Leader

Publikováno v: Protein Engineering, Design and Selection. 30:333-345

Improving an enzyme's initially low catalytic efficiency with a new target substrate by an order of magnitude or two may require only a few rounds of mutagenesis and screening or selection. However, subsequent rounds of optimization tend to yield dec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7fbe9af6bbb4ccfbb9c1ecc174a67615
https://doi.org/10.1093/protein/gzx003

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Single treatment of VX poisoned guinea pigs with the phosphotriesterase mutant C23AL: Intraosseous versus intravenous injection

Autor: Joel L. Sussman, Nidhi Aggarwal, Timo Wille, Moshe Goldsmith, Horst Thiermann, Dan S. Tawfik, Franz Worek, Katharina Neumaier, Christina Ehinger, Marianne Koller, Yacov Ashani

Publikováno v: Toxicology Letters. 258:198-206

The recent attacks with the nerve agent sarin in Syria reveal the necessity of effective countermeasures against highly toxic organophosphorus compounds. Multiple studies provide evidence that a rapid onset of antidotal therapy might be life-saving b

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f99690b31bd8d371d273088446d2add6
https://doi.org/10.1016/j.toxlet.2016.07.004

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The impact of crystallization conditions on structure-based drug design: A case study on the methylene blue/acetylcholinesterase complex

Autor: Joel L. Sussman, Israel Silman, Yechun Xu, Valery L. Shnyrov, Lev Weiner, Clifford E. Felder, Orly Dym, Yacov Ashani, Robbie P. Joosten, Esther Roth, Wanling Song

Publikováno v: Protein Science. 25:1096-1114

Structure-based drug design utilizes apoprotein or complex structures retrieved from the PDB. >57% of crystallographic PDB entries were obtained with polyethylene glycols (PEGs) as precipitant and/or as cryoprotectant, but

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::05dbdacc79a35a3990d5a86d8a476d9c
https://doi.org/10.1002/pro.2923

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