Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Ya-Wen Fu"'
Autor:
Zhi-Xue Yang, Dong-Hao Deng, Zhu-Ying Gao, Zhi-Kang Zhang, Ya-Wen Fu, Wei Wen, Feng Zhang, Xiang Li, Hua-Yu Li, Jian-Ping Zhang, Xiao-Bing Zhang
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-15 (2024)
Abstract The potential for off-target mutations is a critical concern for the therapeutic application of CRISPR-Cas9 gene editing. Current detection methodologies, such as GUIDE-seq, exhibit limitations in oligonucleotide integration efficiency and s
Externí odkaz:
https://doaj.org/article/ca2357b3dbc94140bda92191948be5cd
Autor:
Zhi-Xue Yang, Ya-Wen Fu, Juan-Juan Zhao, Feng Zhang, Si-Ang Li, Mei Zhao, Wei Wen, Lei Zhang, Tao Cheng, Jian-Ping Zhang, Xiao-Bing Zhang
Publikováno v:
Genomics, Proteomics & Bioinformatics, Vol 21, Iss 6, Pp 1206-1220 (2023)
A series of clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9) systems have been engineered for genome editing. The most widely used Cas9 is SpCas9 from Streptococcus pyogenes and SaCas9 from Staphyl
Externí odkaz:
https://doaj.org/article/dd9a845d89064b8db6bec0665d9eb342
Autor:
Wei Wen, Zi-Jun Quan, Si-Ang Li, Zhi-Xue Yang, Ya-Wen Fu, Feng Zhang, Guo-Hua Li, Mei Zhao, Meng-Di Yin, Jing Xu, Jian-Ping Zhang, Tao Cheng, Xiao-Bing Zhang
Publikováno v:
Genome Biology, Vol 22, Iss 1, Pp 1-22 (2021)
Abstract Background After repairing double-strand breaks (DSBs) caused by CRISPR-Cas9 cleavage, genomic damage, such as large deletions, may have pathogenic consequences. Results We show that large deletions are ubiquitous but are dependent on editin
Externí odkaz:
https://doaj.org/article/df51a80f1e864427bde4af68b40acd79
Autor:
Jian-Ping Zhang, Xin-Xin Cheng, Mei Zhao, Guo-Hua Li, Jing Xu, Feng Zhang, Meng-Di Yin, Fei-Ying Meng, Xin-Yue Dai, Ya-Wen Fu, Zhi-Xue Yang, Cameron Arakaki, Ruijun Jeanna Su, Wei Wen, Wen-Tian Wang, Wanqiu Chen, Hannah Choi, Charles Wang, Guangping Gao, Lei Zhang, Tao Cheng, Xiao-Bing Zhang
Publikováno v:
Genome Biology, Vol 20, Iss 1, Pp 1-17 (2019)
Abstract Background Hemophilia A, a bleeding disorder resulting from F8 mutations, can only be cured by gene therapy. A promising strategy is CRISPR-Cas9-mediated precise insertion of F8 in hepatocytes at highly expressed gene loci, such as albumin (
Externí odkaz:
https://doaj.org/article/0175546634cc4b5da1856d314090ad7d
95
The purpose of this study was to validate the Taiwanese version of the Pittsburgh Sleep Quality Index (PSQI-T) and explore the cutoff point for defining sleep disturbance in a sample of 205 Taiwanese patients with multiple diagnoses of cancer
The purpose of this study was to validate the Taiwanese version of the Pittsburgh Sleep Quality Index (PSQI-T) and explore the cutoff point for defining sleep disturbance in a sample of 205 Taiwanese patients with multiple diagnoses of cancer
Externí odkaz:
http://ndltd.ncl.edu.tw/handle/86392076358885478983
Autor:
Juan Fu, Ya-Wen Fu, Juan-Juan Zhao, Zhi-Xue Yang, Si-Ang Li, Guo-Hua Li, Zi-Jun Quan, Feng Zhang, Jian-Ping Zhang, Xiao-Bing Zhang, Chang-Kai Sun
Publikováno v:
Stem Cell Reviews and Reports. 18:1822-1833
Highly efficient gene knockout (KO) editing of CRISPR–Cas9 has been achieved in iPSCs, whereas homology-directed repair (HDR)-mediated precise gene knock-in (KI) and high-level expression are still bottlenecks for the clinical applications of iPSCs
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9049a885066256d78deaf31a0e687d6f
https://doi.org/10.1007/s12015-022-10331-1
https://doi.org/10.1007/s12015-022-10331-1
Autor:
Jing Xu, Mei Zhao, Wei Wen, Meng-Di Yin, Zi-Jun Quan, Tao Cheng, Ya-Wen Fu, Guo-Hua Li, Feng Zhang, Xiao-Bing Zhang, Zhi-Xue Yang, Jian-Ping Zhang, Si-Ang Li
Publikováno v:
Genome Biology, Vol 22, Iss 1, Pp 1-22 (2021)
Genome Biology
Genome Biology
BackgroundAfter repairing double-strand breaks (DSBs) caused by CRISPR-Cas9 cleavage, genomic damage, such as large deletions, may have pathogenic consequences.ResultsWe show that large deletions are ubiquitous but are dependent on editing sites and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a207dbedddc97e3343e53f19f85f6a9
https://doaj.org/article/df51a80f1e864427bde4af68b40acd79
https://doaj.org/article/df51a80f1e864427bde4af68b40acd79
Autor:
Lei Zhang, David J. Baylink, Hongyu Qiu, Hannah Choi, Tao Cheng, Beldon Zhang, Meredith Brown, Feng Zhang, Xin-Yue Dai, Ya-Wen Fu, Zhi-Xue Yang, Wei Wen, Jian-Ping Zhang, Charles Wang, Xiao-Bing Zhang, Wanqiu Chen
Publikováno v:
Science China Life Sciences. 64:1449-1462
Genome-edited human induced pluripotent stem cells (iPSCs) hold great promise for therapeutic applications. However, low editing efficiency has hampered the applications of CRISPR-Cas9 technology in creating knockout and homology-directed repair (HDR
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::aa311882df8f69481a9a969076abcdd8
https://doi.org/10.1007/s11427-020-1855-4
https://doi.org/10.1007/s11427-020-1855-4
Autor:
Xiao-Bing Zhang, Kerby C. Oberg, Feng Zhang, Tao Cheng, Juan-Juan Zhao, Ya-Wen Fu, Lei Zhang, Wei Wen, Wen-Tian Wang, Jian-Ping Zhang, Zhi-Xue Yang, Xin-Yue Dai
Publikováno v:
Nucleic Acids Research
Investigations of CRISPR gene knockout editing profiles have contributed to enhanced precision of editing outcomes. However, for homology-directed repair (HDR) in particular, the editing dynamics and patterns in clinically relevant cells, such as hum
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cc7de84eecfd97c14b89db03e47c530
https://doi.org/10.1093/nar/gkaa1251
https://doi.org/10.1093/nar/gkaa1251
Autor:
Tao Cheng, Xin-Yue Dai, Guo-Hua Li, Xin-Xin Cheng, Fei-Ying Meng, Wen-Tian Wang, Feng Zhang, Jian-Ping Zhang, Ruijun Jeanna Su, Guangping Gao, Wanqiu Chen, Zhi-Xue Yang, Wei Wen, Jing Xu, Hannah Choi, Meng-Di Yin, Charles Wang, Xiao-Bing Zhang, Cameron Arakaki, Ya-Wen Fu, Lei Zhang, Mei Zhao
Publikováno v:
Genome Biology, Vol 20, Iss 1, Pp 1-17 (2019)
Genome Biology
Genome Biology
Background Hemophilia A, a bleeding disorder resulting from F8 mutations, can only be cured by gene therapy. A promising strategy is CRISPR-Cas9-mediated precise insertion of F8 in hepatocytes at highly expressed gene loci, such as albumin (Alb). Unf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cec7d067ef4ed93caf644a5f740f8148
https://doaj.org/article/0175546634cc4b5da1856d314090ad7d
https://doaj.org/article/0175546634cc4b5da1856d314090ad7d