Zobrazeno 1 - 10
of 254
pro vyhledávání: '"Ya-Ming Hou"'
Autor:
Sunita Maharjan, Howard Gamper, Yuka Yamaki, Thomas Christian, Robert Y. Henley, Nan-Sheng Li, Takeo Suzuki, Tsutomu Suzuki, Joseph A. Piccirilli, Meni Wanunu, Erin Seifert, Douglas C. Wallace, Ya-Ming Hou
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-17 (2024)
Abstract Human mitochondrial tRNAs (mt-tRNAs), critical for mitochondrial biogenesis, are frequently associated with pathogenic mutations. These mt-tRNAs have unusual sequence motifs and require post-transcriptional modifications to stabilize their f
Externí odkaz:
https://doaj.org/article/6beaaf5507164908a32932465f77b163
Autor:
Amr Makhamreh, Sepideh Tavakoli, Ali Fallahi, Xinqi Kang, Howard Gamper, Mohammad Nabizadehmashhadtoroghi, Miten Jain, Ya-Ming Hou, Sara H. Rouhanifard, Meni Wanunu
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-13 (2024)
Abstract Chemical modifications to mRNA respond dynamically to environmental cues and are important modulators of gene expression. Nanopore direct RNA sequencing has been applied for assessing the presence of pseudouridine (ψ) modifications through
Externí odkaz:
https://doaj.org/article/e8c24c20290e43c3baa8bfc053081702
Autor:
Isao Masuda, Ya-Ming Hou
Publikováno v:
Frontiers in Microbiology, Vol 15 (2024)
Interpretation of the genetic code from triplets of nucleotides to amino acids is fundamental to life. This interpretation is achieved by cellular tRNAs, each reading a triplet codon through its complementary anticodon (positions 34–36) while deliv
Externí odkaz:
https://doaj.org/article/e7999c18706a4df0bab901e5090e3fc7
Autor:
Mateusz Jedrzejewski, Barbara Belza, Iwona Lewandowska, Marta Sadlej, Agata P. Perlinska, Rafal Augustyniak, Thomas Christian, Ya-Ming Hou, Marcin Kalek, Joanna I. Sulkowska
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 21, Iss , Pp 3999-4008 (2023)
The Nep1 protein is essential for the formation of eukaryotic and archaeal small ribosomal subunits, and it catalyzes the site-directed SAM-dependent methylation of pseudouridine (Ψ) during pre-rRNA processing. It possesses a non–trivial topology,
Externí odkaz:
https://doaj.org/article/fce9cd31689d48fc924328112827005f
Autor:
Sepideh Tavakoli, Mohammad Nabizadeh, Amr Makhamreh, Howard Gamper, Caroline A. McCormick, Neda K. Rezapour, Ya-Ming Hou, Meni Wanunu, Sara H. Rouhanifard
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-12 (2023)
Pseudouridine (psi) is an RNA modification that can affect its physiology, including increased half-life. Here the authors identify sites of psi modification in the human transcriptome using direct RNA sequencing and provide a “ground truth” list
Externí odkaz:
https://doaj.org/article/0d5179a76ab04c11a24ee5827b1cd49c
Autor:
Isao Masuda, Ya-Ming Hou
Publikováno v:
STAR Protocols, Vol 4, Iss 2, Pp 102196- (2023)
Summary: We describe here a genome-wide screening approach to identify the most critical core reaction among a network of many that are supported by an essential gene to establish cell viability. We describe steps for maintenance plasmid construction
Externí odkaz:
https://doaj.org/article/d227eb1c038446f6b2165a56186e9a8c
Interplay between an ATP-binding cassette F protein and the ribosome from Mycobacterium tuberculosis
Autor:
Zhicheng Cui, Xiaojun Li, Joonyoung Shin, Howard Gamper, Ya-Ming Hou, James C. Sacchettini, Junjie Zhang
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-12 (2022)
The authors present structures of the MtbEttA protein bound to or free of the ribosome from Mycobacterium tuberculosis under different nucleotide states, which provide insights into the mechanism of translational regulation by EttA-like proteins.
Externí odkaz:
https://doaj.org/article/a1ceaf8c8b90485fbe8e0f72e313307d
Autor:
Christine E. Carbone, Anna B. Loveland, Howard B. Gamper, Ya-Ming Hou, Gabriel Demo, Andrei A. Korostelev
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
EF-G drives ribosomal translocation along mRNA. Time-resolved cryo-EM captured translocation with EF-G•GTP—without inhibitors—revealing how EF-G uses ribosome fluctuations to drive translocation and GTP hydrolysis to leave at the right moment.
Externí odkaz:
https://doaj.org/article/4bea5e8abd3d4ecd81046f7ade0b17b2
Autor:
Gabriel Demo, Howard B. Gamper, Anna B. Loveland, Isao Masuda, Christine E. Carbone, Egor Svidritskiy, Ya-Ming Hou, Andrei A. Korostelev
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Translational frameshifting is a mechanism that expands the coding capabilities of mRNA. Here, structures of 70S ribosome complexes with GTPase elongation factor G (EF-G), a +1-frameshifting-prone mRNA and tRNAs reveal the cooperation between the rib
Externí odkaz:
https://doaj.org/article/9e96fbbefbf946cca2e64e5aa10017b3
Autor:
Howard Gamper, Haixing Li, Isao Masuda, D. Miklos Robkis, Thomas Christian, Adam B. Conn, Gregor Blaha, E. James Petersson, Ruben L. Gonzalez, Ya-Ming Hou
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-18 (2021)
Genome recoding with quadruplet codons requires a +1-frameshift-suppressor tRNA able to insert an amino acid at quadruplet codons of interest. Here the authors identify the mechanisms resulting in +1 frameshifting and the steps of the elongation cycl
Externí odkaz:
https://doaj.org/article/f25e7830b887458f96ecdebbbd086160