Zobrazeno 1 - 10
of 74
pro vyhledávání: '"Y V, Sheludko"'
Publikováno v:
Transgenic research. 31(3)
Cytochrome P450 monooxygenases (CYPs) are important tools for regio- and stereoselective oxidation of target molecules or engineering of metabolic pathways. Functional heterologous expression of eukaryotic CYPs is often problematic due to their depen
Autor:
V. V. Kleschevnikov, L. O. Sakhno, I. A. Arbuzova, N. V. Kuchuk, I. M. Gerasymenko, Y. V. Sheludko, Victor E. Dosenko, V. R. Kedlian
Publikováno v:
Cytology and Genetics. 51:1-7
Development of RNAi-based therapeutics is a fast growing field of the pharmaceutical industry. Using plants for production of pharmaceutically valuable siRNAs may have significant advantages of costeffectiveness, scalability, and low risk of contamin
Publikováno v:
Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv. 14:181-186
Aim. For modifying of the plant organisms with genetic engineering techniques to produce genus stress resistant low temperatures or frosts it is necessary to check their physiological characteristics at high temperatures stress. Methods. In this stud
Autor:
T. N. Kyrpa, I. V. Goldenkova-Pavlova, A. M. Ostapchuk, T. A. Hadjiev, Y. V. Sheludko, L. A. Sakhno, I. M. Gerasymenko
Publikováno v:
Russian Journal of Plant Physiology. 62:283-291
We established transgenic lines of Nicotiana tabacum expressing hybrid genes of Synechocystis sp. PCC 6803 Δ12 (desA) acyllipid desaturase and Synechococcus vulcanus Δ9 (desC) acyllipid desaturase with or without sequence coding for transit peptide
Autor:
Y. V. Sheludko, I. M. Gerasymenko
Publikováno v:
Biotechnology letters. 39(7)
To exploit cold-inducible biochemical processes beneficial for foreign mRNA transcription, translation and storage, as well as protein product stability, during Agrobacterium-mediated transient expression. The efficiency of three different 5′-regul
Autor:
V. A. Rudas, I. M. Gerasymenko, A. A. Klebanovych, T. M. Klein, Y. V. Sheludko, N. V. Kuchuk, A. M. Shakhovsky
Publikováno v:
Transgenic research. 26(1)
The development of tools which ensure the desired level of transgene expression in plastids is a prerequisite for the effective utilization of these plant organelles for the deployment of bioactive proteins. High-level accumulation of target proteins
Publikováno v:
Biopolymers and Cell, Vol 28, Iss 5, Pp 368-373 (2012)
Our aim was to investigate the influence of some exogenous agents on the recombinant protein accumulation in plants via Agrobacterium-mediated transient expression. Methods. Agrobacterium-mediated transient expression method, spectrophotometric metho
Publikováno v:
Scopus-Elsevier
During the last decade interferons are regarded as potent candidates for generation of plant-based edible vaccines because of broad spectrum of antiviral activities and adjuvant properties. Establishment and certification of numerous interferon produ
Autor:
Y. V. Sheludko, Y. R. Sindarovska, N. V. Kuchuk, N. Y. Spivak, I. M. Gerasymenko, Z. M. Olevinskaya
Publikováno v:
Cytology and Genetics. 44:313-316
Human interferon alpha2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized conditions resulted in m
Autor:
V. Momot, N. N. Cherep, N. V. Kuchuk, Y. V. Sheludko, V. A. Rudas, O. O. Ovcharenko, I. K. Komarnitsky
Publikováno v:
Plant Cell, Tissue and Organ Culture (PCTOC). 105:21-27
Asymmetric intergeneric hybrid plants were obtained through protoplast fusion between Orychophragmus violaceus (L.) O.E. Schulz and Lesquerella fendleri (Gray) Wats. The latter carried chloroplasts transformed with the fused aadA16gfp gene construct,