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Autor:
D G, Kamenev, Y N, Shkryl, G N, Veremeichik, V A, Golotin, N N, Naryshkina, Y O, Timofeeva, S N, Kovalchuk, I V, Semiletova, V P, Bulgakov
Publikováno v:
Journal of nanoscience and nanotechnology. 15(12)
The cDNA fragment encoding the catalytic domain of the new silicatein-like cathepsin enzyme LoCath was expressed in a strain Top10 of Escherichia coli, extracted and purified via nickel-affinity chromatography. Recombinant enzyme performed silica-pol