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Autor:
M M, Zaripov, G V, Afanasieva, X A, Glukhova, Y A, Trizna, A S, Glukhov, I P, Beletsky, O V, Prusakova
Publikováno v:
Bioorganicheskaia khimiia. 41(4)
A simple and fast method for obtaining biotin-labeled monoclonal antibodies was developed usingcontent of hybridoma culture supernatant sufficient to select antibody pairs in sandwich ELISA. The method consists in chemical biotinylation of antigen-bo