Exploring d-xylose oxidation in Saccharomyces cerevisiae through the Weimberg pathway

Autor: Lisa Wasserstrom, Diogo Portugal-Nunes, Henrik Almqvist, Anders G. Sandström, Gunnar Lidén, Marie F. Gorwa-Grauslund

Publikováno v: AMB Express, Vol 8, Iss 1, Pp 1-16 (2018)

Abstract Engineering of the yeast Saccharomyces cerevisiae towards efficient d-xylose assimilation has been a major focus over the last decades since d-xylose is the second most abundant sugar in nature, and its conversion into products could signifi

Externí odkaz: https://doaj.org/article/3af36dd99b3540efa18c7fddadf1a68d

Engineering Escherichia coli for glycolic acid production from D-xylose through the Dahms pathway and glyoxylate bypass

Autor: Kristine Rose M. Ramos, Grace M. Nisola, Angelo B. Bañares, Won-Keun Lee, Kris Niño G. Valdehuesa, Rhudith B. Cabulong, Wook-Jin Chung

Publikováno v: Applied Microbiology and Biotechnology. 102:2179-2189

Glycolic acid (GA) is an ⍺-hydroxy acid used in cosmetics, packaging, and medical industries due to its excellent properties, especially in its polymeric form. In this study, Escherichia coli was engineered to produce GA from D-xylose by linking th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e8bd89b6820f477137356c0c4f7028c
https://doi.org/10.1007/s00253-018-8744-8

Characterization and mutagenesis of two novel iron–sulphur cluster pentonate dehydratases

Autor: Paul Carlson, Martina Andberg, Merja Oja, Anu Koivula, Merja Penttilä, Sophie Bozonnet, Niina Hakulinen, Niina Aro-Kärkkäinen, Mervi Toivari, Michael J. O’Donohue

Publikováno v: Applied Microbiology and Biotechnology
Applied Microbiology and Biotechnology, 2016, 100 (17), pp.7549-7563. ⟨10.1007/s00253-016-7530-8⟩
Applied Microbiology and Biotechnology, Springer Verlag, 2016, 100 (17), pp.7549-7563. ⟨10.1007/s00253-016-7530-8⟩
Andberg, M, Aro-Kärkkäinen, N, Carlson, P, Oja, M, Bozonnet, S, Toivari, M, Hakulinen, N, O'Donohue, M, Penttilä, M & Koivula, A 2016, ' Characterization and mutagenesis of two novel iron-sulphur cluster pentonate dehydratases ', Applied Microbiology and Biotechnology, vol. 100, no. 17, pp. 7549-7563 . https://doi.org/10.1007/s00253-016-7530-8

We describe here the identification and characterization of two novel enzymes belonging to the IlvD/EDD protein family, the D-xylonate dehydratase from Caulobacter crescentus, Cc XyDHT, (EC 4.2.1.82), and the L-arabonate dehydratase from Rhizobium le

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce6f9bb3a1d54cb5f1d8cb65e681e742
https://doi.org/10.1007/s00253-016-7530-8

Crystallization and X-ray diffraction analysis of an L-arabinonate dehydratase from Rhizobium leguminosarum bv. trifolii and a D-xylonate dehydratase from Caulobacter crescentus

Autor: Martina Andberg, Mohammad Mubinur Rahman, Juha Rouvinen, Anu Koivula, Nina Hakulinen

Publikováno v: 'Acta Crystallographica F ', vol: 72, pages: 604-608 (2016)
Acta Crystallographica. Section F, Structural Biology Communications
Rahman, M M, Andberg, M, Koivula, A, Rouvinen, J & Hakulinen, N 2016, ' Crystallization and X-ray diffraction analysis of an l-arabinonate dehydratase from Rhizobium leguminosarum bv. trifolii and a d-xylonate dehydratase from Caulobacter crescentus ', Acta Crystallographica Section F: Structural Biology Communications, vol. 72, no. Part 8, pp. 604-608 . https://doi.org/10.1107/S2053230X16010311

l-Arabinonate dehydratase and d-xylonate dehydratase from the IlvD/EDD family were crystallized by the vapour-diffusion method. Diffraction data sets were collected to resolutions of 2.40 and 2.66 Å from crystals of l-arabinonate dehydratase and d-x

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::82c80c1293545cd1c36f0187ac78747a
https://epn-library.esrf.fr/flora/jsp/index_view_direct_anonymous.jsp?record=doc:PUB_ESRF:44939

Metabolic pathway optimization for biosynthesis of 1,2,4-butanetriol from xylose by engineered Escherichia coli

Autor: Jinbao Wang, Yang Zhang, Haijun Gao, Nannan Zhang

Publikováno v: Enzyme and microbial technology.

1,2,4-Butanetriol (BT) and related derivatives have been widely used in many fields, especially in the military and in medicine. In this paper, we systematically optimized the BT biosynthetic pathway. We first investigated the activities of various N

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ef31e714166b3b568849d67a1ae201d
https://pubmed.ncbi.nlm.nih.gov/27702485