Next generation sequencing is a highly reliable method to analyze exon 7 deletion of survival motor neuron 1 (SMN1) gene

Autor: Sumin Zhao, Yaoshen Wang, Xiuqing Xin, Zhonghai Fang, Linlin Fan, Zhiyu Peng, Rui Han, Chaonan Shi, Yixiang Zhang, Chuang Fan, Jun Sun, Xuelian He

Publikováno v: Scientific Reports, Vol 12, Iss 1, Pp 1-9 (2022)

Abstract Spinal muscular atrophy (SMA) is one of the most common and severe genetic diseases. SMA carrier screening is an effective way to identify couples at risk of having affected children. Next-generation sequencing (NGS)-based expanded carrier s

Externí odkaz: https://doaj.org/article/06470a79b1c34fe3bb6ba6eadd8ccd96

Development of an autotrophic fermentation technique for the production of fatty acids using an engineered Ralstonia eutropha cell factory

Autor: Zhongkang, Li, Bin, Xiong, Li, Liu, Siwei, Li, Xiuqing, Xin, Zhi, Li, Xueli, Zhang, ChangHao, Bi

Publikováno v: Journal of Industrial Microbiology and Biotechnology. 46:783-790

Massive emission of CO2 into atmosphere from consumption of carbon deposit is causing climate change. Researchers have applied metabolic engineering and synthetic biology techniques for improving CO2 fixation efficiency in many species. One solution

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c975387fb76ea53f3ac6606d782324b3
https://doi.org/10.1007/s10295-019-02156-8

Glycosylase base editors enable C-to-A and C-to-G base changes

Autor: Marcus A. Price, Ju Li, Susan J. Rosser, Muzi Hu, Siwei Li, Changhao Bi, Dongdong Zhao, Xiuqing Xin, Xueli Zhang

Publikováno v: Zhao, D, Li, J, Li, S, Xin, X, Hu, M, Price, M A, Rosser, S J, Bi, C & Zhang, X 2020, ' Glycosylase base editors enable C-to-A and C-to-G base changes ', Nature Biotechnology, vol. 39, pp. 35-40 . https://doi.org/10.1038/s41587-020-0592-2

Current base editors (BEs) catalyze only base transitions (C to T and A to G) and cannot produce base transversions. Here we present BEs that cause C-to-A transversions in Escherichia coli and C-to-G transversions in mammalian cells. These glycosylas

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21a85ef8966a5ef9ed337eba981a07db
https://www.pure.ed.ac.uk/ws/files/192864573/GlycosylaseBaseEditors.pdf

Double-Check Base Editing for Efficient A to G Conversions

Autor: Siwei Li, Feiyu Fan, Zhongkang Li, Qianwen Xie, Xueli Zhang, Changhao Bi, Ju Li, Dongdong Zhao, Xiuqing Xin

Publikováno v: ACS synthetic biology. 8(12)

With the development of CRISPR/Cas9 technology, a new generation of editing methods that convert specific bases has enabled precise single-base mutations. To date, conversion of cytosine to thymidine and adenine to guanine has been achieved using the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb113a166e2f494bd10b4dd6cfd3756d
https://pubmed.ncbi.nlm.nih.gov/31765564

Publisher Correction: Glycosylase base editors enable C-to-A and C-to-G base changes

Autor: Siwei Li, Muzi Hu, Dongdong Zhao, Ju Li, Xiuqing Xin, Susan J. Rosser, Changhao Bi, Marcus A. Price, Xueli Zhang

Publikováno v: Nature Biotechnology. 39:115-115

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a39559f85b7caa27a124ebda9d7499d3
https://doi.org/10.1038/s41587-020-0648-3