Cryo-EM reveals the molecular basis oflaminin polymerization and LN-lamininopathies

Autor: Arkadiusz W. Kulczyk, Karen K. McKee, Ximo Zhang, Iwona Bizukojc, Ying Q. Yu, Peter D. Yurchenco

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-7 (2023)

In this work the authors report the cryo-EM structure of the laminin polymer node and reveal the molecular basis of calcium-dependent formation of laminin lattice. The work provides insights into laminin polymerization defects manifesting in human di

Externí odkaz: https://doaj.org/article/60769db0fb56402eb40aadb01cc00d25

Optimized Reversed-Phase Liquid Chromatography/Mass Spectrometry Methods for Intact Protein Analysis and Peptide Mapping of Adeno-Associated Virus Proteins

Autor: Weibin Chen, Ximo Zhang, Ying Qing Yu, Stephan M. Koza, Xiaoying Jin, Lin Liu, Zichuang Zhang

Publikováno v: Human Gene Therapy

Recombinant adeno-associated viruses (AAVs) have emerged as the leading gene delivery platform owing to their nonpathogenic nature and long-term gene expression capability. The AAV capsid, in addition to protecting the viral genome, plays an importan

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::783a0808ebc8293291e44687ee37267f
https://pubmed.ncbi.nlm.nih.gov/34278837

Released N-Glycan Analysis for Biotherapeutic Development Using Liquid Chromatography and Mass Spectrometry

Autor: Ximo, Zhang

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2261

In this chapter, we describe an LC-fluorescence (FLR)/MS-based method for released N-glycan analysis in the development of biotherapeutic proteins. The method includes enzymatic release and labeling of N-glycans with a signal-enhancing tag, LC-MS dat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::bcbd8ccad62571cf4deb39361a6178ad
https://pubmed.ncbi.nlm.nih.gov/33420983

Routine Analysis of N-Glycans Using Liquid Chromatography Coupled to Routine Mass Detection

Autor: Mitul Patel, Ximo Zhang, Vithiya Vimalraj

Publikováno v: Methods in Molecular Biology ISBN: 9781071612408

Analysis of N-glycans are commonly conducted via enzymatic release, labeling, and liquid chromatography (LC) separation and fluorescent detection. Mass spectrometry (MS) has been increasingly used as an orthogonal detection method to provide addition

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::dc3e0e9ae475afe807074dea8b05e143
https://doi.org/10.1007/978-1-0716-1241-5_15

Tissue residues and withdrawal time of moxidectin treatment of swine by topical pour-on application.

Autor: Jicheng, Qiu, Hongzhi, Xiao, Pan, Sun, Yuying, Cao, Qinyao, Wu, Ximo, Zhang, Lu, Zhang, Xiaohui, Gong, Xingyuan, Cao, Feifei, Sun

Publikováno v: Food Additives & Contaminants. Part A: Chemistry, Analysis, Control, Exposure & Risk Assessment; Apr2021, Vol. 38 Issue 4, p602-608, 7p, 2 Charts, 3 Graphs