Výsledky vyhledávání - "Xiaoping Z. He"

Akademický článek

Development of an icIEF assay for monitoring AAV capsid proteins and application to gene therapy products

Autor: Xiaoping Z. He, Thomas W. Powers, Sisi Huang, Zhenjiu Liu, Heliang Shi, John D. Orlet, Jim J. Mo, Saipraveen Srinivasan, Steven Jacobs, Kun Zhang, Herbert A. Runnels, Melissa M. Anderson, Thomas F. Lerch

Publikováno v: Molecular Therapy: Methods & Clinical Development, Vol 29, Iss , Pp 133-144 (2023)

Adeno-associated virus (AAV) gene therapy vectors, which contain a DNA transgene packaged into a protein capsid, have shown tremendous therapeutic potential in recent years. Methods traditionally used in quality control labs, such as high-performance

Externí odkaz: https://doaj.org/article/19963ef7905e47ad85ca83d2661c2bbd

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Charge heterogeneity profiling of monoclonal antibodies using low ionic strength ion-exchange chromatography and well-controlled pH gradients on monolithic columns

Autor: Qian Wang, Paul R. Haddad, Xiaoping Z. He, Anna Nordborg, Nathan A. Lacher, Mohammad Talebi, Andras Gaspar, Emily F. Hilder

Publikováno v: Journal of Chromatography A. 1317:148-154

In this work, the suitability of employing shallow pH gradients generated using single component buffer systems as eluents through cation-exchange (CEX) monolithic columns is demonstrated for the high-resolution separation of monoclonal antibody (mAb

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c87a561db07208b15ef961ea5e99334c
https://doi.org/10.1016/j.chroma.2013.08.061

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Analysis of charge heterogeneities in mAbs using imaged CE

Autor: Jim J. Mo, Amy H. Que, Xiaoping Z. He

Publikováno v: Electrophoresis. 30(5)

An analytical method using the imaged CE (iCE) technology has been developed and validated for measuring contents of charge variants for mAb molecules. The method could generate similar information that require both conventional IEF electrophoresis a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2502e11728849ecb8e82c1a84e0e92e4
https://pubmed.ncbi.nlm.nih.gov/19199293

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Characterization of monoclonal antibodies using polymeric cation exchange monoliths in combination with salt and pH gradients

Autor: Bo Zhang, Xiaoping Z. He, Emily F. Hilder, Anna Nordborg, Paul R. Haddad

The characterization of three different mAb formulations of pharmaceutical interest is demonstrated using cation exchange polymethacrylate-based monolithic columns as well as imaged CE (iCE). Elution of the mAbs from both a strong cation exchanger (P

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::77e95bd6630c9550f0ce28405de2013b
https://hdl.handle.net/11541.2/111905

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