Zobrazeno 1 - 10
of 131
pro vyhledávání: '"Xiang-Fu Wu"'
Autor:
Yaozhou Zhang, Zheng-Bing Lv, Bo Liu, Qingliang Zheng, Xiang-Fu Wu, Hongxiu Ge, Jianqing Chen, Zuoming Nie, Jianhong Shu, Jian Chen
Publikováno v:
African Journal of Biotechnology; Vol 10, No 14 (2011); 2588-2599
Leucine-rich repeat (LRR) proteins play important roles in the transduction of cellular signals and activation of defense responses. By scanning the cDNA library of silkworm ( Bombyx mori ) pupae constructed in our laboratory, we identified a 1557 bp
Autor:
Jiang Wang, Jian Chen, Yaozhou Zhang, Xuedong Wang, Zhengbing Lv, Jianqing Chen, Xiang-Fu Wu, Zuoming Nie, Dan Wang, Gai Qijing
Publikováno v:
Cell and Tissue Research. 331:725-738
We have cloned and sequenced a novel Bombyx mori gene that encodes a protein having a high degree of homology with other known troponin C (TnC) proteins. The amino acid sequence, DX[DN]X[DSG]X(6)E, a highly conserved putative Ca(2+) -binding motif fo
Autor:
Zuoming Nie, Jian Chen, Dan Wang, Yaozhou Zhang, Lingyin Kong, Hongdan Shen, Xuedong Wang, Xiang-Fu Wu, Zhengbing Lv
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 1770:1598-1604
14-3-3 proteins, which have been identified in a wide variety of eukaryotes, are highly conserved acidic proteins. In this study, we identified two genes in silkworm that encode 14-3-3 proteins (Bm14-3-3zeta and Bm14-3-3epsilon). Category of two 14-3
Publikováno v:
Molecular Biotechnology. 35:179-184
Osteoprotegerin (OPG) regulates the formation of osteoclasts and is involved in the regulation of bone resorption and remodeling. To investigate the feasibility of using silkworm (Bombyx mori) larvae to produce recombinant osteoprotegerin as a oral a
Autor:
Hongdan Shen, Jian Chen, Xuedong Wang, Yaozhou Zhang, Zuoming Nie, Xue-Jian Wang, Dan Wang, Zhengbing Lv, Xiang-Fu Wu
Publikováno v:
Journal of Cellular Biochemistry. 102:970-979
Cellular retinoic acid binding protein (CRABP) is a member of intracellular lipid-binding protein (iLBP), and closely associated with retinoic acid (RA) activity. We have cloned the CRABP gene from silkworm pupae and studied the interaction between B
Publikováno v:
Acta Biochimica et Biophysica Sinica. 38:201-206
Human lactoferrin (hLf) is a multifunctional iron-binding glycoprotein. In this study, we amplified hLf cDNA by reverse transcription-polymerase chain reaction from normal human mammary gland. The nucleotide sequence of the hLf was identical to the k
Publikováno v:
Journal of Biotechnology. 118:246-256
In this paper, recombinant human lactoferrin (rhLf) was expressed very well using Bombyx mori nuclear polyhedrosis baculovirus expression system. Infection of silkworm larvae with recombinant virus, vBm-hLf, the rhLf was efficiently secreted into lar
Publikováno v:
Acta Biochimica et Biophysica Sinica. 37:371-378
Methionine synthase (MS) is grouped into two classes. Class One MS (MetH) and Class Two MS (MetE) share no homology and differ in their catalytic model. Based on the conserved sequences of metE genes from different organisms, a segment of the metE ge
Publikováno v:
Acta Biochimica et Biophysica Sinica
The causative agent of severe acute respiratory syndrome (SARS) is a previously unidentified coronavirus, SARS-CoV. The nucleocapsid (N) protein of SARS-CoV is a major viral protein recognized by acute and early convalescent sera from SARS patients.
Publikováno v:
Protein & Peptide Letters. 11:63-69
We constructed a mouse PC6B truncated mutant and introduced a tag of 6 consecutive histidines at its carboxyl terminus for simple purification. Using the baculovirus expression system and standard enzymatic assay, we obtained recombinant mouse PC6B p