Zobrazeno 1 - 10
of 529
pro vyhledávání: '"XhoI"'
Publikováno v:
Journal of Shahrekord University of Medical Sciences, Vol 23, Iss 2, Pp 68-75 (2021)
Background and aims: Human interferon beta-1a (hIFNβ-1a) is a 22.5-kDa glycoprotein used to treat diseases such as multiple sclerosis (MS). Because of appropriate post-translation modifications, protein isolation, and lack of toxicity in Chinese ham
Autor:
He Zhiyuan, Ming-Li Wang, Wu Bo, Dong-Mei Gao, Jun Zhao, Xia Bingbing, Hai-Yang Yu, Zhou Wei, Jiang Minzhi
Publikováno v:
Journal of Veterinary Research, Vol 65, Iss 1, Pp 101-108 (2021)
Journal of Veterinary Research
Journal of Veterinary Research
Introduction Ovine interferon-tau (oIFN-τ) is a newly discovered type I interferon. This study used biochemical techniques to transform the oIFN-τ gene into Escherichia coli to obtain the mass and soluble expression of the recombinant protein. Mate
Publikováno v:
YAKUGAKU ZASSHI. 140:1501-1508
In Japan, mitragynine, 7-hydroxymitragynine and Mitragyna speciosa Korth. (M. speciosa, "Kratom") were controlled as Designated Substances under the Pharmaceutical and Medical Device Act from March 2016. In this study, the origins of 16 Kratom produc
Publikováno v:
BioTechniques. 69:161-170
Endolysins have been studied intensively as an alternative to antibiotics. In this study, endolysin derived from a phage which infects methicillin-resistant Staphylococcus aureus (MRSA) was cloned and expressed in Escherichia coli pET28a. Initially,
Publikováno v:
International Journal of Peptide Research and Therapeutics. 26:2355-2364
Cellobiose is one of the most abundant carbohydrates available in nature which can be used as biofuel in various applications such as food industry, energy production, paper production, etc. with no harm to the environment. One way for a safe decompo
Publikováno v:
The Analyst. 145:6054-6060
Nucleases play a crucial role in DNA replication, recombination and repair which are associated with cancers. Herein, we develop a four-color fluorescent probe for ratiometric detection of multiple nucleases. This four-color fluorescent probe consist
Publikováno v:
The Journal of Poultry Science, Vol 41, Iss 2, Pp 147-154 (2004)
We investigated the efficiency of DNA extraction and the accuracy of polymerase chain reaction- (PCR-) based sexing of DNA that was obtained from adult chickens and early chicken embryos. DNA samples were prepared using water-, ammonium-, and protein
Externí odkaz:
https://doaj.org/article/781dff2b7a684df69a93b71cc973db90
Publikováno v:
Iranian Biomedical Journal
Iranian Biomedical Journal, Vol 24, Iss 1, Pp 15-23 (2020)
Iranian Biomedical Journal, Vol 24, Iss 1, Pp 15-23 (2020)
Background Streptokinase (SK), a heterogeneous plasminogen activator (PA) protein from groups A, C, and G streptococci (GAS, GCS, GGS, respectively) contains three structural domains (SKα, SKβ, and SK). Based on the variable region of SKβ, GAS-
Autor:
Hirofumi Yoshikawa, Mitsuhiro Itaya, Mitsuru Sato, Rintaro Sato, Masaru Tomita, Satoru Watanabe
Publikováno v:
The Journal of Biochemistry. 166:231-236
Bacillus subtilis 168 has been explored as a platform for the synthesis and transmission of large DNA. Two inherent DNA incorporation systems, natural transformation and pLS20-based conjugation transfer, enable rapid handling of target DNA. Both syst
Publikováno v:
Journal of immunological methods. 494
Antibody phage display technology plays an important role in the development of monoclonal antibodies, humanization, and affinity evolution of antibodies. Thus far, antibody phage display mainly focuses on the display of antibody variable region or a