A Recombinant Human Anti-Platelet scFv Antibody Produced in Pichia pastoris for Atheroma Targeting.

Autor: Amelie Vallet-Courbin, Mélusine Larivière, Agnès Hocquellet, Audrey Hemadou, Sarjapura-Nagaraja Parimala, Jeanny Laroche-Traineau, Xavier Santarelli, Gisèle Clofent-Sanchez, Marie-Josée Jacobin-Valat, Abdelmajid Noubhani

Publikováno v: PLoS ONE, Vol 12, Iss 1, p e0170305 (2017)

Cells of the innate and adaptive immune system are key factors in the progression of atherosclerotic plaque, leading to plaque instability and rupture, potentially resulting in acute atherothrombotic events such as coronary artery disease, cerebrovas

Externí odkaz: https://doaj.org/article/dea397549106467ca89ec9d1c18744c1

Media Selection in Ion Exchange Chromatography in a Single Microplate

Autor: Charlotte Cabanne, Xavier Santarelli

Publikováno v: Methods in Molecular Biology ISBN: 9781071607749
Methods in Molecular Biology ISBN: 9781627039765
Protein Downstream Processing. Methods in Molecular Biology
Protein Downstream Processing. Methods in Molecular Biology, 2178, Springer, pp.27-33, 2021, 978-1-0716-0775-6. ⟨10.1007/978-1-0716-0775-6_4⟩

High-throughput process development is more and more used in chromatography. Limitations are the tools provided by the manufacturers. Here, we describe a method to select ion exchange chromatographic media using a 96-well filter microplate.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8752140941a6e083fd3a20d9af5b3ed
https://doi.org/10.1007/978-1-0716-0775-6_4

Development of anti-chloro (192) tyrosine HDL apoA-I antibodies for the immunodiagnosis of cardiovascular diseases

Autor: Gilles Joucla, Marie-Josée Jacobin-Valat, Xavier Santarelli, Krishnan Venkataraman, Rajasekar R. Prasanna, Mookambeswaran A. Vijayalakshmi, N. S. Jayaprakash, Kirubanandhan Lokeshwaran, Wilfrid Dieryck, Audrey Hemadou, Gisèle Clofent-Sanchez

Publikováno v: Journal of Immunological Methods
Journal of Immunological Methods, Elsevier, 2019, 474, pp.112637. ⟨10.1016/j.jim.2019.112637⟩

High density lipoproteins (HDL) are considered cardio protective. Apolipoprotein A-I (apoA-I), a major component of HDL helps in reverse cholesterol transport, whose function is greatly affected during atherosclerosis due to oxidation by myeloperoxid

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6720732196e11f43b4efa9e17d193fb5
http://hdl.handle.net/20.500.12278/7555

CPPS

Autor: Xavier Santarelli, Tsutomu Arakawa

Publikováno v: Current Protein & Peptide Science
Current Protein & Peptide Science, Bentham Science, 2018, 20 (1), pp.3-3
Current Protein and Peptide Science
Current Protein and Peptide Science, Bentham Science Publishers, In press, 20 (1), pp.3-3. ⟨10.2174/138920372001181031110923⟩

Mixed-mode (multimodal) chromatography now occupies an important place in biopharmaceutical purification. It adds a new dimension to such conventional chromatographies as ion-exchange, hydrophobic interaction, reverse-phase and size-exclusion. Mixed-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34b0fd901b031d1e9cea939cce628cf6
http://hdl.handle.net/20.500.12278/3838

Mixed Mode Chromatography, Complex Development for Large Opportunities

Autor: Charlotte Cabanne, Xavier Santarelli

Publikováno v: Current Protein and Peptide Science
Current Protein and Peptide Science, Bentham Science Publishers, In press, 20 (1), pp.22-27. ⟨10.2174/1389203718666171030104211⟩

Mixed mode chromatography resins with salt tolerance, large design space and orthogonal selectivity requires a slightly more complex development than traditional resins. It is important to screen several ligands and several binding and elution condit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ddf1ba52864c3b4b36c7a8918904cc9d
https://hal.archives-ouvertes.fr/hal-02503996