Výsledky vyhledávání - "Xavier Ponte"

Towards optimal substrate feeding for heterologous protein production in Pichia pastoris (Komagataella spp) fed-batch processes under P AOX1 control: a modeling aided approach

Autor: Michael Maurer, Francisco Valero, José Luis Montesinos-Seguí, José Manuel Barrigón, Diethard Mattanovich, Xavier Ponte

Publikováno v: Journal of Chemical Technology & Biotechnology. 93:3208-3218

BACKGROUND: To improve the efficiency of a bioprocess, key parameters, such as yield, titer and productivity, must be considered. They are mainly dependent on the specific rates for product (qP) and cell growth (μ), and their correlation determines

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f191796cd41492566707198d5e9f6ed7
https://doi.org/10.1002/jctb.5677

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Bioprocess efficiency in Rhizopus oryzae lipase production by Pichia pastoris under the control of PAOX1 is oxygen tension dependent

Autor: José Luis Montesinos-Seguí, Xavier Ponte, Francisco Valero

Publikováno v: Process Biochemistry. 51:1954-1963

Dissolved oxygen (DO) has been stated as a key parameter for Pichia pastoris (Mut+) cultures growing on methanol. DO set point (DOSP) – between 5 and 50% air saturation – with time-varying oxygen transfer rate (OTR) was investigated during the in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::fd6120b6b3b2ce70669f994041238e2f
https://doi.org/10.1016/j.procbio.2016.08.030

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Functional expression of aryl‐alcohol oxidase in Saccharomyces cerevisiae and Pichia pastoris by directed evolution

Autor: Javier Viña-Gonzalez, Katarina Elbl, Xavier Ponte, Miguel Alcalde, Francisco Valero

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
instname

Aryl‐alcohol oxidase (AAO) plays a fundamental role in the fungal ligninolytic secretome, acting as a supplier of H2O2. Despite its highly selective mechanism of action, the presence of this flavooxidase in different biotechnological settings has h

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65d1aedbb0419615eda86e5991ab5cf2
http://hdl.handle.net/10261/169223

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Inflow occlusion

Autor: Sousa, Xavier Ponte de

Publikováno v: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP

Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2014 Submitted by Biblioteca da Faculdade de Medicina da Universidade de Lisboa (repositorio@medicina.ulisboa.pt) on 2016-07-26T14:41:12Z No. of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______3056::78764b908425f43eadfe57c6e483901b

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Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae lipase

Autor: Johannes Hemmerich, Xavier Ponte, Astrid Hörmann, Francisco Valero, Núria Adelantado, José Manuel Barrigón, Pau Ferrer, Frank Kensy

Publikováno v: Microbial Cell Factories
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona

In Pichia pastoris bioprocess engineering, classic approaches for clone selection and bioprocess optimization at small/micro scale using the promoter of the alcohol oxidase 1 gene (P AOX1 ), induced by methanol, present low reproducibility leading to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::798944e22c93bf721f91051898f5e90a
https://pubmed.ncbi.nlm.nih.gov/24606982

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