Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Xavier Garrabou"'
Autor:
Duncan Stuart Macdonald, Donald Hilvert, Cindy Klaus, Xavier Garrabou, Takahiro Mori, Rebecca Verez
Publikováno v:
Journal of the American Chemical Society. 142:10250-10254
Controlling regio- and stereoselectivity of aldol additions is generally challenging. Here we show that an artificial aldolase with high specificity for acetone as the aldol donor can be reengineered via single active site mutations to accept linear
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a09e8bd946a64fbec8e052f7cd2914a1
https://doi.org/10.1021/jacs.0c02351
https://doi.org/10.1021/jacs.0c02351
Publikováno v:
Journal of the American Chemical Society. 139:103-106
Artificial enzymes created by computational design and directed evolution are versatile biocatalysts whose promiscuous activities represent potentially attractive starting points for divergent evolution in the laboratory. The artificial aldolase RA95
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13bb66689b5157183ba17788584923bb
https://doi.org/10.1021/jacs.6b11928
https://doi.org/10.1021/jacs.6b11928
Publikováno v:
Chemistry - A European Journal. 23:6001-6003
The promiscuity of de novo designed enzymes provides a privileged platform for diverse abiological reactions. In this work, we report the first example of a nitroolefin synthase that catalyzes the Henry condensation between aromatic aldehydes and nit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6386145335cc8894a02bc27d267132b9
https://doi.org/10.1002/chem.201605757
https://doi.org/10.1002/chem.201605757
Publikováno v:
Angewandte Chemie International Edition. 54:5609-5612
Recent advances in computational design have enabled the development of primitive enzymes for a range of mechanistically distinct reactions. Here we show that the rudimentary active sites of these catalysts can give rise to useful chemical promiscuit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::589ecf4c0fe109341d560e714fec70eb
https://doi.org/10.1002/anie.201500217
https://doi.org/10.1002/anie.201500217
Publikováno v:
Journal of the American Chemical Society. 138:6972-6974
The simple catalytic motifs utilized by enzymes created by computational design and directed evolution constitute a potentially valuable source of chemical promiscuity. Here we show that the artificial retro-aldolase RA95.5-8 is able to use a reactiv
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b80f35ba8f8eb52a93a70801d5f6ff84
https://doi.org/10.1021/jacs.6b00816
https://doi.org/10.1021/jacs.6b00816
Publikováno v:
Angewandte Chemie (International ed. in English). 57(19)
Enzymes are valuable biocatalysts for asymmetric synthesis due to their exacting stereocontrol. Changing the selectivity of an existing catalyst for new applications is, however, challenging. Here we show that, in contrast, the stereoselectivity of a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5700470a83f9425bd2a87752e9623cd5
https://pubmed.ncbi.nlm.nih.gov/29446221
https://pubmed.ncbi.nlm.nih.gov/29446221
Publikováno v:
Current opinion in structural biology. 48
Exploring the sequence space of enzyme catalysts is ultimately a numbers game. Ultrahigh-throughput screening methods for rapid analysis of millions of variants are therefore increasingly important for investigating sequence-function relationships, s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b3d3220212e6418f2e759c865b87ae1
https://pubmed.ncbi.nlm.nih.gov/29413955
https://pubmed.ncbi.nlm.nih.gov/29413955
Autor:
Jordi Bujons, Mariana L. Gutierrez, Teodor Parella, Xavier Garrabou, Jesús Joglar, Anna Soler, Pere Clapés, Eduardo Busto, Karel Hernández
Publikováno v:
Advanced Synthesis & Catalysis. 356:3007-3024
A multistep chemoenzymatic synthesis for stereodiverse polyhydroxypipecolic acid analogues, homoiminocyclitols and polyhydroxylated piperidine and pyrrolidine derivatives combining glycine-dependent aldolases and both D-fructose-6-phosphate aldolase
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ad6105a66d77ae67d67c9f45853cfbc0
https://doi.org/10.1002/adsc.201400453
https://doi.org/10.1002/adsc.201400453
Autor:
Andrew D. Griffiths, David Baker, Peer R. E. Mittl, Richard Obexer, Xavier Garrabou, Donald Hilvert, Alexei Godina
Publikováno v:
Nature chemistry. 9(1)
Designing catalysts that achieve the rates and selectivities of natural enzymes is a long-standing goal in protein chemistry. Here, we show that an ultrahigh-throughput droplet-based microfluidic screening platform can be used to improve a previously
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00ca7fb71891deb44aedc8346b6fd08b
https://pubmed.ncbi.nlm.nih.gov/27995916
https://pubmed.ncbi.nlm.nih.gov/27995916
Autor:
Wolf-Dieter Fessner, Anna Szekrenyi, Pere Clapés, Xavier Garrabou, Sebastian Junker, Deniz Güclü, Michael Kickstein
Publikováno v:
Digital.CSIC. Repositorio Institucional del CSIC
instname
instname
Application of aldolases for the asymmetric synthesis of multifunctional chiral products is hampered by their reputed strict nucleophile (=aldol donor) specificity owing to a mechanistic requirement for creating a carbanion nucleophile in aqueous med
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e39a42dd8720936c236a53fb3d8f017
http://hdl.handle.net/10261/129488
http://hdl.handle.net/10261/129488
