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Influence of reagent formulation on mRNA quantification by RT-PCR using imported external standard curves

Autor: Mireia Farriol, Xavi Orta

Publikováno v: Acta Biochimica Polonica. 52:845-848

Use of an imported external standard curve is common in real-time quantitative RT-PCR. Two practical strategies for long-term experiments include importing a grand mean standard curve to all accumulated runs or using daily imported standard curves, f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a4d9cc769de4720971a1793e932143a
https://doi.org/10.18388/abp.2005_3396

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Role of putrescine in cell proliferation in a colon carcinoma cell line

Autor: Yusimy Venereo, Mireia Farriol, Jose Ma Castellanos, Toni Segovia-Silvestre, Xavi Orta

Publikováno v: Nutrition. 17:934-938

OBJECTIVES: Putrescine, the precursor for higher polyamine biosynthesis, is necessary for cell growth in mammals. Ornithine decarboxylase (ODC) activity and polyamine production are increased in neoplastic cells. Using colon cancer cell line derived

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d91025894337ffa404e3e7746242c8a
https://doi.org/10.1016/s0899-9007(01)00670-0

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Antioxidant effect of polyamines on erythrocyte cell membrane lipoperoxidation after free-radical damage

Autor: Yusimy Venereo, Mireia Farriol, Xavi Orta, Toni Segovia-Silvestre

Publikováno v: Phytotherapy research : PTR. 17(1)

This in vitro study determined the effect of three doses each (100, 500 and 1000 microm) of putrescine, spermidine and spermine on malondialdehyde (MDA) release in red blood cells (RBCs) from healthy individuals after hydrogen peroxide stimulation (1

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::562a3b859aad842851e523c1baf6abac
https://pubmed.ncbi.nlm.nih.gov/12557246

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Quantitative analysis of ornithine decarboxylase mRNA by reverse transcription-PCR with the LightCycler system

Autor: Xavi Orta, Mireia Farriol, Yusimy Venereo, Jorge Olsina, Joaquin Balsells, Toni Segovia-Silvestre

Publikováno v: Clinical chemistry. 48(10)

Polyamines originate from the decarboxylation of ornithine, which gives rise to putrescine, the first polyamine in this metabolic pathway. The reaction is catalyzed by ornithine decarboxylase (ODC; EC 4.1.1.17), a rate-limiting enzyme in polyamine sy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::552aae8f0429fe764f39aad9c42e5778
https://pubmed.ncbi.nlm.nih.gov/12324496

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