Engineering Yarrowia lipolytica to produce glycoproteins homogeneously modified with the universal Man3GlcNAc2 N-glycan core.

Autor: Karen De Pourcq, Petra Tiels, Annelies Van Hecke, Steven Geysens, Wouter Vervecken, Nico Callewaert

Publikováno v: PLoS ONE, Vol 7, Iss 6, p e39976 (2012)

Yarrowia lipolytica is a dimorphic yeast that efficiently secretes various heterologous proteins and is classified as "generally recognized as safe." Therefore, it is an attractive protein production host. However, yeasts modify glycoproteins with no

Externí odkaz: https://doaj.org/article/f27fc7f5be924bc6a6bbebf5e66cbe4f

Contribution of Galactofuranose to the Virulence of the Opportunistic Pathogen Aspergillus fumigatus

Autor: Gerhard H. Braus, Sven Krappmann, Armin Braun, Roland Contreras, Philipp S. Schmalhorst, Wouter Vervecken, Manfred Rohde, Meike Müller, Françoise H. Routier, Hans Bakker

Publikováno v: EUKARYOTIC CELL

The filamentous fungus Aspergillus fumigatus is the primary cause of invasive aspergillosis, an often fatal condition affecting people with a weakened immune system. Along with the immunocompromised population, the incidence of invasive aspergillosis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::159aefb6f1f5a9c40c9c0ae8d41620a4
https://doi.org/10.1128/ec.00109-08

Rating of CCl4-induced rat liver fibrosis by blood serum glycomics

Autor: Cuiying Chen, Bernard de Hemptinne, Marleen Praet, Ye-Dong Fan, Tomasz Jaworski, Liesbeth Desmyter, Wouter Vervecken, Roland Contreras

Publikováno v: Journal of Gastroenterology and Hepatology. 22:1148-1154

Background: Non-invasive staging of human liver fibrosis is a desirable objective that remains under extensive evaluation. Animal model systems are often used for studying human liver disease and screening antifibrotic compounds. The aim of the prese

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b622173f0775bcb7eb5ccabf6093b597
https://doi.org/10.1111/j.1440-1746.2006.04553.x

Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88

Autor: Marc J. E. C. van der Maarel, Marco A. van den Berg, Steven Geysens, Etienne Danchin, Rachel M. van der Kaaij, Hein Stam, Jibin Sun, Kaj Albermann, Maurien M.A. Olsthoorn, Arnold J. M. Driessen, Frans M. Klis, Monika Schmoll, Gerald Hofmann, K. Pal, Thomas Guillemette, Bernard Henrissat, Johannes H. de Winde, David B. Archer, Coenie Goosen, Pedro M. Coutinho, Richard Albang, Michael Cornell, Jens Nielsen, Johannes Andries Roubos, Harrie J. Kools, Peter J.J. Van De Vondervoort, Cornelis Maria Jacobus Sagt, Noël Nicolaas Maria Elisabeth Van Peij, Jannick Dyrløv Bendtsen, Alard Van Dijk, Marga Herweijer, Martin Mortimer, Ursula Rinas, An-Ping Zeng, Roland Contreras, Holger Wedler, Stephen G. Oliver, Cees A. M. J. J. van den Hondel, David W. Ussery, Gert S.P. Groot, Jaap Visser, Mikael Rørdam Andersen, Arthur F. J. Ram, Hildegard Henna Menke, René T. J. M. van der Heijden, Paul S. Dyer, Piet W. J. de Groot, Han A. B. Wösten, Alfons J. M. Debets, Jacques A.E. Benen, János Varga, Lubbert Dijkhuizen, Christian P. Kubicek, Peter J. T. Dekker, Albert J. J. van Ooyen, Johannes Petrus Theodorus Wilhelmus Van Den Hombergh, Ronald P. de Vries, Rogier Meulenberg, Jürgen Lauber, Mark X. Caddick, Geoffrey Turner, Xin Lu, Patricia Ann van Kuyk, Wouter Vervecken, Peter J. Schaap, Piet W.M. van Dijck, Stefaan Breestraat, Herman Jan Pel, Christophe d'Enfert

Publikováno v: Nature Biotechnology, 25(2), 221-231
Nature Biotechnology
Nature Biotechnology, Nature Publishing Group, 2007, 25 (2), pp.221-231. ⟨10.1038/nbt1282⟩
Nature Biotechnology, 25(2), 221-231. Nature Publishing Group
Nature Biotechnology, 2007, 25 (2), pp.221-231. ⟨10.1038/nbt1282⟩
Nature Biotechnology 25 (2007)
Nature Biotechnology, 25, 221-31
Nature Biotechnology, 25, 2, pp. 221-31
Nature Biotechnology, 25, 221-231
Nature Biotechnology, 25(2), 221. Nature Publishing Group
Technical University of Denmark Orbit

Contains fulltext : 51722.pdf (Publisher’s version ) (Closed access) The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec3d8832d6c3443d190409999a1cdf16
https://doi.org/10.1038/nbt1282

In Vivo Synthesis of Mammalian-Like, Hybrid-Type N-Glycans in Pichia pastoris

Autor: Roland Contreras, Wouter Vervecken, Nico Callewaert, Steven Geysens, Vladimir Kaigorodov, Kristof De Vusser

Publikováno v: Applied and Environmental Microbiology. 70:2639-2646

The Pichia pastoris N-glycosylation pathway is only partially homologous to the pathway in human cells. In the Golgi apparatus, human cells synthesize complex oligosaccharides, whereas Pichia cells form mannose structures that can contain up to 40 ma

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::92688e87a202e4a002840c9b6de4195b
https://doi.org/10.1128/aem.70.5.2639-2646.2004