A high-resolution melt curve toolkit to identify lineage-defining SARS-CoV-2 mutations

Autor: Fraser, AJ, Greenland-Bews, C, Kelly, D, Williams, CT, Bengey, D, Buist, K, Clerkin, K, Finch, LS, Gould, S, Kontogianni, K, Savage, HR, Thompson, CR, Wardale, J, Watkins, RL, Wooding, D, Allen, AJ, Body, R, Braybrook, J, Buckle, P, Clark, E, Dark, P, Davis, K, Gordon, A, Hayward, G, Halstead, A, Harden, C, Inkson, C, Jones, N, Jones, W, Lasserson, D, Lee, J, Lendrem, C, Lewington, A, Logan, M, Micocci, M, Nicholson, B, Perera-Salazar, R, Prestwich, G, Price, A, Reynard, C, Riley, B, Simpson, AJ, Tate, V, Turner, P, Wilcox, M, Zhifang, M, Adams, ER, Atienzar, AC, Edwards, T, Allen, DJ

Publikováno v: Fraser, A J, Greenland-Bews, C, Kelly, D, Williams, C T, CONDOR Steering Group, LSTM Diagnostics Group, Body, R, Adams, E R, Cubas Atienzar, A, Edwards, T, Allen, D J & Dark, P 2023, ' A high-resolution melt curve toolkit to identify lineage-defining SARS-CoV-2 mutations ', Scientific Reports, vol. 13, no. 1, 3887 . https://doi.org/10.1038/s41598-023-30754-1

The emergence of severe acute respiratory syndrome 2 (SARS-CoV-2) variants of concern (VOCs), with mutations linked to increased transmissibility, vaccine escape and virulence, has necessitated the widespread genomic surveillance of SARS-CoV-2. This

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30dae00c4947d5c0f84dd0f74c8d4dad
http://hdl.handle.net/10044/1/105266

Development of a sensitive, quantitative assay with broad subtype specificity for detection of total HIV-1 nucleic acids in plasma and PBMC

Autor: Kibirige, C. N., Manak, M., King, D., Abel, B., Hack, H., Wooding, D., Liu, Y., Fernandez, N., Dalel, J., Kaye, Steve, Imami, N., Jagodzinski, L., Gilmour, J.

Publikováno v: Scientific Reports, Vol 12, Iss 1, Pp 1-16 (2022)
Scientific Reports

An LTR-based quantitative PCR (qPCR) assay was modified and optimized for the quantification of total HIV-1 nucleic acids in plasma and PBMC. TaqMan qPCR primers and probes were designed against the NCBI/LANL HIV-1 compendium database by analyzing se

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c71ae7cac54c7b9671a836827cec88ac
https://doi.org/10.1038/s41598-021-03016-1

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