Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Wolfram Hemmer"'
Publikováno v:
LWT - Food Science and Technology. 31:664-667
The genetically modified corn `MaisGardTM' (Monsanto, `YieldGardTM' in the U.S.A.) is resistant to the European corn borer due to the insertion and expression of the cryIA(b) gene fromBacillus thuringiensisssp. kurstaki. This gene controlled by a 35S
Publikováno v:
Biochemistry. 37:7708-7715
The conserved glycines in the glycine-rich loop (Leu-Gly50-Thr-Gly52-Ser-Phe-Gly55-Arg-Val) of the catalytic (C) subunit of cAMP-dependent protein kinase were each mutated to Ser (G50S, G52S, and G55S). The effects of these mutations were assessed he
Publikováno v:
Journal of Biological Chemistry. 272:16946-16954
A glycine-rich loop in the ATP-binding site is one of the most highly conserved sequence motifs in protein kinases. Each conserved glycine (Gly-50, Gly-52, and Gly-55) in the catalytic (C) subunit of cAMP-dependent protein kinase (cAPK) was replaced
Recombinant Strategies for Rapid Purification of Catalytic Subunits of cAMP-Dependent Protein Kinase
Publikováno v:
Analytical Biochemistry. 245:115-122
Knowledge of the crystal structure of the catalytic subunit (C) of cAMP-dependent protein kinase provided for the first time a molecular basis for probing function by site-directed mutagenesis. The purification of mutant C-subunits, however, presente
Autor:
Wolfram Hemmer, Wolfram Kutsch
Publikováno v:
Journal of Insect Physiology. 40:519-525
In adult and last larval stage locusts, muscle responses to short wind puffs (8–1000 ms) were recorded. For adult animals the characteristic flight motor output can be released in both pterothoracic segments. For the last larval stage in which thes
Autor:
Wolfram Hemmer, Theo Wallimann
Publikováno v:
Molecular and Cellular Biochemistry. :193-220
Over the past years, a concept for creatine kinase function, the 'PCr-circuit' model, has evolved. Based on this concept, multiple functions for the CK/PCr-system have been proposed, such as an energy buffering function, regulatory functions, as well
Autor:
Hans M. Eppenberger, Wolfram Hemmer, Elizabeth M. Furter-Graves, Else Zanolla, Theo Wallimann
Publikováno v:
European Journal of Neuroscience. 6:538-549
Creatine kinase isoenzymes were localized in the chicken cerebellum by the use of isoenzyme-specific anti-chicken creatine kinase antibodies. Brain-type creatine kinase was found in high amounts in the molecular layer, particularly in Bergmann glial
Autor:
Wolfram Kutsch, Wolfram Hemmer
Publikováno v:
Journal of Insect Physiology. 40:97-106
The tritocerebral commissure giant (TCG) wind-sensitive interneurone is involved in initiation and maintenance of flight in adult locusts. In this paper we study this interneurone in the imago and non-flying larval instars. Cobalt fills show that the
Publikováno v:
Developmental Neuroscience. 15:261-270
The physiological role of the phosphocreatine (PCr)/creatine kinase (CK) system has been studied in rat brain by comparing maturational changes in in vivo CK-catalyzed reaction rate and activities of CK isoenzymes. The CK-catalyzed reaction rates, me
Publikováno v:
FEBS Letters. 283:37-43
Isoenzymes of creatine kinase (CK, EC 2.7.3.2) in guinea-pig smooth, cardiac and skeletal muscles as well as in brain were analyzed by cellulose acetate electrophoresis and FPLC gel permeation chromatography. In crude tissue extracts of smooth muscle