Zobrazeno 1 - 10
of 118
pro vyhledávání: '"Wolfgang Fischle"'
Autor:
Baolei Yuan, Chongwei Bi, Yeteng Tian, Jincheng Wang, Yiqing Jin, Khaled Alsayegh, Muhammad Tehseen, Gang Yi, Xuan Zhou, Yanjiao Shao, Fernanda Vargas Romero, Wolfgang Fischle, Juan Carlos Izpisua Belmonte, Samir Hamdan, Yanyi Huang, Mo Li
Publikováno v:
BMC Biology, Vol 22, Iss 1, Pp 1-15 (2024)
Abstract Background CRISPR-Cas9 genome editing often induces unintended, large genomic rearrangements, posing potential safety risks. However, there are no methods for mitigating these risks. Results Using long-read individual-molecule sequencing (ID
Externí odkaz:
https://doaj.org/article/d1681b87d8e041b8a7a543e11f0f1746
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-12 (2023)
Abstract In CRISPR-Cas and related nuclease-mediated genome editing, target recognition is based on guide RNAs (gRNAs) that are complementary to selected DNA regions. While single site targeting is fundamental for localized genome editing, targeting
Externí odkaz:
https://doaj.org/article/fc029be41a704115a2fd7ef30fd63382
Autor:
Karthik Eswara, Wolfgang Fischle
Publikováno v:
ACS Central Science, Vol 8, Iss 2, Pp 146-149 (2022)
Externí odkaz:
https://doaj.org/article/4c9d9ef82d51475ebbecfd5c9bbc7ee4
Autor:
Alexandra Stützer, Luisa M. Welp, Monika Raabe, Timo Sachsenberg, Christin Kappert, Alexander Wulf, Andy M. Lau, Stefan-Sebastian David, Aleksandar Chernev, Katharina Kramer, Argyris Politis, Oliver Kohlbacher, Wolfgang Fischle, Henning Urlaub
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
Cross-linking mass spectrometry (XLMS) allows mapping of protein-protein and protein-RNA interactions, but the analysis of protein-DNA complexes remains challenging. Here, the authors develop a UV light-based XLMS workflow to determine protein-DNA in
Externí odkaz:
https://doaj.org/article/88c57b0f820b4a1e9b22493af84520da
Autor:
Kyoko Hiragami-Hamada, Szabolcs Soeroes, Miroslav Nikolov, Bryan Wilkins, Sarah Kreuz, Carol Chen, Inti A. De La Rosa-Velázquez, Hans Michael Zenn, Nils Kost, Wiebke Pohl, Aleksandar Chernev, Dirk Schwarzer, Thomas Jenuwein, Matthew Lorincz, Bastian Zimmermann, Peter Jomo Walla, Heinz Neumann, Tuncay Baubec, Henning Urlaub, Wolfgang Fischle
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-16 (2016)
Heterochromatin protein 1 (HP1), including HP1 α, β and γ, is a family of non-histone chromatin factors thought to be involved in chromatin organization. Here, the authors show that dimeric HP1β interacts dynamically with H3K9me3, a hallmark of h
Externí odkaz:
https://doaj.org/article/daad9f601a9746829955629c5cc1bf39
Autor:
Philip Yuk Kwong Yung, Alexandra Stuetzer, Wolfgang Fischle, Anne-Marie Martinez, Giacomo Cavalli
Publikováno v:
Cell Reports, Vol 11, Iss 9, Pp 1437-1445 (2015)
Trimethylation at histone H3K27 is central to the polycomb repression system. Juxtaposed to H3K27 is a widely conserved phosphorylatable serine residue (H3S28) whose function is unclear. To assess the importance of H3S28, we generated a Drosophila H3
Externí odkaz:
https://doaj.org/article/109f44103f274ef88cb16acb9e9659b4
Autor:
Efrat Shema-Yaacoby, Miroslav Nikolov, Mahmood Haj-Yahya, Peter Siman, Eric Allemand, Yuki Yamaguchi, Christian Muchardt, Henning Urlaub, Ashraf Brik, Moshe Oren, Wolfgang Fischle
Publikováno v:
Cell Reports, Vol 4, Iss 3, Pp 601-608 (2013)
Chromatin posttranslational modifications (PTMs), including monoubiquitylation of histone H2B on lysine 120 (H2Bub1), play a major role in regulating genome functions. To elucidate the molecular mechanisms of H2Bub1 activity, a chromatin template uni
Externí odkaz:
https://doaj.org/article/616a77e55b9e4f359fcf1122fff351f8
Autor:
Huaying Zhao, Rodolfo Ghirlando, Carlos Alfonso, Fumio Arisaka, Ilan Attali, David L Bain, Marina M Bakhtina, Donald F Becker, Gregory J Bedwell, Ahmet Bekdemir, Tabot M D Besong, Catherine Birck, Chad A Brautigam, William Brennerman, Olwyn Byron, Agnieszka Bzowska, Jonathan B Chaires, Catherine T Chaton, Helmut Cölfen, Keith D Connaghan, Kimberly A Crowley, Ute Curth, Tina Daviter, William L Dean, Ana I Díez, Christine Ebel, Debra M Eckert, Leslie E Eisele, Edward Eisenstein, Patrick England, Carlos Escalante, Jeffrey A Fagan, Robert Fairman, Ron M Finn, Wolfgang Fischle, José García de la Torre, Jayesh Gor, Henning Gustafsson, Damien Hall, Stephen E Harding, José G Hernández Cifre, Andrew B Herr, Elizabeth E Howell, Richard S Isaac, Shu-Chuan Jao, Davis Jose, Soon-Jong Kim, Bashkim Kokona, Jack A Kornblatt, Dalibor Kosek, Elena Krayukhina, Daniel Krzizike, Eric A Kusznir, Hyewon Kwon, Adam Larson, Thomas M Laue, Aline Le Roy, Andrew P Leech, Hauke Lilie, Karolin Luger, Juan R Luque-Ortega, Jia Ma, Carrie A May, Ernest L Maynard, Anna Modrak-Wojcik, Yee-Foong Mok, Norbert Mücke, Luitgard Nagel-Steger, Geeta J Narlikar, Masanori Noda, Amanda Nourse, Tomas Obsil, Chad K Park, Jin-Ku Park, Peter D Pawelek, Erby E Perdue, Stephen J Perkins, Matthew A Perugini, Craig L Peterson, Martin G Peverelli, Grzegorz Piszczek, Gali Prag, Peter E Prevelige, Bertrand D E Raynal, Lenka Rezabkova, Klaus Richter, Alison E Ringel, Rose Rosenberg, Arthur J Rowe, Arne C Rufer, David J Scott, Javier G Seravalli, Alexandra S Solovyova, Renjie Song, David Staunton, Caitlin Stoddard, Katherine Stott, Holger M Strauss, Werner W Streicher, John P Sumida, Sarah G Swygert, Roman H Szczepanowski, Ingrid Tessmer, Ronald T Toth, Ashutosh Tripathy, Susumu Uchiyama, Stephan F W Uebel, Satoru Unzai, Anna Vitlin Gruber, Peter H von Hippel, Christine Wandrey, Szu-Huan Wang, Steven E Weitzel, Beata Wielgus-Kutrowska, Cynthia Wolberger, Martin Wolff, Edward Wright, Yu-Sung Wu, Jacinta M Wubben, Peter Schuck
Publikováno v:
PLoS ONE, Vol 10, Iss 5, p e0126420 (2015)
Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose o
Externí odkaz:
https://doaj.org/article/3bd91e8f42734f39965350067490e003
Autor:
Francesca Munari, Nasrollah Rezaei-Ghaleh, Shengqi Xiang, Wolfgang Fischle, Markus Zweckstetter
Publikováno v:
PLoS ONE, Vol 8, Iss 4, p e60887 (2013)
As essential components of the molecular machine assembling heterochromatin in eukaryotes, HP1 (Heterochromatin Protein 1) proteins are key regulators of genome function. While several high-resolution structures of the two globular regions of HP1, ch
Externí odkaz:
https://doaj.org/article/94f925db5f664815a27736b73ce193fd
Publikováno v:
PLoS Genetics, Vol 7, Iss 3, p e1002017 (2011)
MBT domain proteins are involved in developmental processes and tumorigenesis. In vitro binding and mutagenesis studies have shown that individual MBT domains within clustered MBT repeat regions bind mono- and dimethylated histone lysine residues wit
Externí odkaz:
https://doaj.org/article/e480656fb71a4f62a7eac90c6ee328ce