Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Wolf Holtkamp"'
Autor:
Alessandro Ascione, Marcello Belfiore, Jaana Vesterinen, Mihaela Buda, Wolf Holtkamp, Francesca Luciani
Publikováno v:
mAbs, Vol 16, Iss 1 (2024)
ABSTRACTTherapeutic mAbs show a specific “charge fingerprint” that may affect safety and efficacy, and, as such, it is often identified as a critical quality attribute (CQA). Capillary iso-electric focusing (cIEF), commonly used for the evaluatio
Externí odkaz:
https://doaj.org/article/90b4b78ca43542ea9001ea85f6de584f
Publikováno v:
PLoS Computational Biology, Vol 20, Iss 3, p e1011918 (2024)
Processive enzymes like polymerases or ribosomes are often studied in bulk experiments by monitoring time-dependent signals, such as fluorescence time traces. However, due to biomolecular process stochasticity, ensemble signals may lack the distinct
Externí odkaz:
https://doaj.org/article/c4cdb5a848f940708a48139066417690
Autor:
Kai-Hsin Chan, Valentyn Petrychenko, Claudia Mueller, Cristina Maracci, Wolf Holtkamp, Daniel N. Wilson, Niels Fischer, Marina V. Rodnina
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Rescue of ribosomes stalled on non-stop mRNA is essential for cell viability, and several rescue systems to resolve stalling exist in bacteria. Here, the authors use rapid kinetics and cryo-EM to reveal the pathway and selectivity mechanism of ArfB-m
Externí odkaz:
https://doaj.org/article/2ee13ea8233d47ba89c88cbd83274f16
Autor:
Sarah Adio, Heena Sharma, Tamara Senyushkina, Prajwal Karki, Cristina Maracci, Ingo Wohlgemuth, Wolf Holtkamp, Frank Peske, Marina V Rodnina
Publikováno v:
eLife, Vol 7 (2018)
Release factors RF1 and RF2 promote hydrolysis of peptidyl-tRNA during translation termination. The GTPase RF3 promotes recycling of RF1 and RF2. Using single molecule FRET and biochemical assays, we show that ribosome termination complexes that carr
Externí odkaz:
https://doaj.org/article/b5b4525df75141a792bec2900ba14734
Autor:
Claudia Mueller, Daniel N. Wilson, Wolf Holtkamp, Marina V. Rodnina, Valentyn Petrychenko, Kai-Hsin Chan, Niels Fischer, Cristina Maracci
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Nature Communications
Nature Communications, 11:4106
Nature Communications
Nature Communications, 11:4106
Alternative ribosome-rescue factor B (ArfB) rescues ribosomes stalled on non-stop mRNAs by releasing the nascent polypeptide from the peptidyl-tRNA. By rapid kinetics we show that ArfB selects ribosomes stalled on short truncated mRNAs, rather than o
Publikováno v:
Nucleic Acids Research
The bacterial signal recognition particle (SRP) is part of the machinery that targets ribosomes synthesizing membrane proteins to membrane-embedded translocons co-translationally. Recognition of nascent membrane proteins occurs by virtue of a hydroph
Autor:
Joerg Mittelstaet, Anton A. Komar, Goran Kokic, Wolf Holtkamp, Marina V. Rodnina, Marcus Jäger
Publikováno v:
Science
Protein domains can fold into stable tertiary structures while they are synthesized on the ribosome. We used a high-performance, reconstituted in vitro translation system to investigate the folding of a small five-helix protein domain—the N-termina
Author response: Dynamics of ribosomes and release factors during translation termination in E. coli
Autor:
Prajwal Karki, Ingo Wohlgemuth, Frank Peske, Cristina Maracci, Marina V. Rodnina, Sarah Adio, Wolf Holtkamp, Tamara Senyushkina, Heena Sharma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d1ce00e4b20c720b06351ba5da5d3832
https://doi.org/10.7554/elife.34252.023
https://doi.org/10.7554/elife.34252.023
Autor:
Wolf Holtkamp, Marina V. Rodnina, Frank Peske, Cristina Maracci, Ingo Wohlgemuth, Sarah Adio, Tamara Senyushkina, Heena Sharma, Prajwal Karki
Publikováno v:
eLife
eLife, Vol 7 (2018)
eLife, Vol 7 (2018)
Release factors RF1 and RF2 promote hydrolysis of peptidyl-tRNA during translation termination. The GTPase RF3 promotes recycling of RF1 and RF2. Using single molecule FRET and biochemical assays, we show that ribosome termination complexes that carr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0805162b5b84d6cbe76b5d0a8f90cbae
https://doi.org/10.1101/243485
https://doi.org/10.1101/243485
Autor:
Marija Liutkute, Marina V. Rodnina, Ekaterina Samatova, Manisankar Maiti, Jörg Enderlein, Wolf Holtkamp
Publikováno v:
Biophysical Journal. 116:189a-190a
The ribosome synthesizes proteins according to the sequence of the messenger RNA (mRNA) by progressively adding amino acids to the C-terminus of the nascent peptide. For a protein to become fully functional, it has to fold into a specific three-dimen