Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Wojciech M. Śmigiel"'
Autor:
Tjeerd Pols, Hendrik R. Sikkema, Bauke F. Gaastra, Jacopo Frallicciardi, Wojciech M. Śmigiel, Shubham Singh, Bert Poolman
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Functional out-of-equilibrium networks are typical of living cells. Here the authors report the construction of a sustained ATP production system in vesicles with controlled energy dissipation and physicochemical homeostasis.
Externí odkaz:
https://doaj.org/article/978bc8a91a0141beb7a7d957b10b5695
Autor:
Luca Mantovanelli, Dmitrii S Linnik, Michiel Punter, Hildeberto Jardón Kojakhmetov, Wojciech M Śmigiel, Bert Poolman
Publikováno v:
PLoS Computational Biology, Vol 19, Iss 9, p e1011093 (2023)
We have developed Simulation-based Reconstructed Diffusion (SbRD) to determine diffusion coefficients corrected for confinement effects and for the bias introduced by two-dimensional models describing a three-dimensional motion. We validate the metho
Externí odkaz:
https://doaj.org/article/d3c2fcba44e24a918f2fdaf7b688ea26
Publikováno v:
eLife, Vol 6 (2017)
Much of the molecular motion in the cytoplasm is diffusive, which possibly limits the tempo of processes. We studied the dependence of protein mobility on protein surface properties and ionic strength. We used surface-modified fluorescent proteins (F
Externí odkaz:
https://doaj.org/article/1b0b600116e3495bb1b7b16e695ef1f3
Autor:
Wojciech M, Śmigiel, Luca, Mantovanelli, Dmitrii S, Linnik, Michiel, Punter, Jakob, Silberberg, Limin, Xiang, Ke, Xu, Bert, Poolman
Publikováno v:
Science advances. 8(32)
We analyze the structure of the cytoplasm by performing single-molecule displacement mapping on a diverse set of native cytoplasmic proteins in exponentially growing
Autor:
Wojciech M. Śmigiel, Luca Mantovanelli, Dmitrii S. Linnik, Michiel Punter, Jakob Silberberg, Limin Xiang, Ke Xu, Bert Poolman
Publikováno v:
Science Advances, 8(32):eabo5387. AMER ASSOC ADVANCEMENT SCIENCE
We analyze the structure of the cytoplasm by performing single-molecule displacement mapping on a diverse set of native cytoplasmic proteins in exponentially growing Escherichia coli . We evaluate the method for application in small compartments and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15a4f57f0920268782afecbf9bc0b27a
https://research.rug.nl/en/publications/5d982d55-7fa7-4eb9-bbc1-eeaa4b386334
https://research.rug.nl/en/publications/5d982d55-7fa7-4eb9-bbc1-eeaa4b386334
Publikováno v:
Emerging Topics in Life Sciences
The bottom-up construction of synthetic cells from molecular components is arguably one of the most challenging areas of research in the life sciences. We review the impact of confining biological systems in synthetic vesicles. Complex cell-like syst
Publikováno v:
eLife, Vol 6 (2017)
eLife
eLife, 6:eLife.30084. ELIFE SCIENCES PUBLICATIONS LTD
eLife
eLife, 6:eLife.30084. ELIFE SCIENCES PUBLICATIONS LTD
Much of the molecular motion in the cytoplasm is diffusive, which possibly limits the tempo of processes. We studied the dependence of protein mobility on protein surface properties and ionic strength. We used surface-modified fluorescent proteins (F