Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Wlodzimierz Mandecki"'
Publikováno v:
Nucleic Acids Research. 12:4011-4017
Synthetic oligonucleotides were used to introduce mutations into the lacPUV5 promoter. Four mutations were obtained at positions -13, -14, and -15, with respect to the transcriptional start site. The effects of these mutations were measured in vivo a
Publikováno v:
Biochemical and Biophysical Research Communications. 93:223-227
Summary Complementation has been found to occur between extracts of lacZ X90, an operator distal nonsense mutant of E. coli , and CNBr24,the 32-residue carboxyl-terminal cyanogen bromide peptide from β-galactosidase. The degree of complementation is
Autor:
Wlodzimierz Mandecki, Jadwiga Wild
Publikováno v:
Molecular and General Genetics MGG. 173:339-343
Three temperature-sensitive mutant strains for RNA polymerase beta or beta' subunits (carrying mutations tsx, A2R7 and R120) were used in order to investigate the dependence of the induced lac expression on stimulation by cyclic AMP after the shift t
Publikováno v:
Nucleic Acids Research. 10:903-912
A lac promotor mutant was constructed by filling in the protruding ends of the HpaII site located within the lac promotor. The mutation, named M42, is a two base pair insertion that changes the distance between the -10 and -35 regions from 18 to 20 r