A unique human cord blood CD8 ⁺ CD45RA ⁺ CD27 ⁺ CD161 ⁺ T-cell subset identified by flow cytometric data analysis using Seurat.

Autor: Reyes JGA; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.; Nepean Hospital, Nepean Blue Mountains Local Health District, Penrith, New South Wales, Australia.; Nepean Clinical School, The University of Sydney, Sydney, New South Wales, Australia., Ni D; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.; Nepean Hospital, Nepean Blue Mountains Local Health District, Penrith, New South Wales, Australia.; Nepean Clinical School, The University of Sydney, Sydney, New South Wales, Australia., Santner-Nanan B; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.; Nepean Hospital, Nepean Blue Mountains Local Health District, Penrith, New South Wales, Australia.; Nepean Clinical School, The University of Sydney, Sydney, New South Wales, Australia., Pinget GV; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.; Nepean Clinical School, The University of Sydney, Sydney, New South Wales, Australia., Kraftova L; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.; Nepean Clinical School, The University of Sydney, Sydney, New South Wales, Australia.; Department of Microbiology, Faculty of Medicine, University Hospital in Pilsen, Charles University, Pilsen, Czech Republic.; Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic., Ashhurst TM; Sydney Cytometry Core Research Facility, Charles Perkins Centre, The University of Sydney and Centenary Institute, Sydney, New South Wales, Australia., Marsh-Wakefield F; Liver Injury and Cancer Program, Centenary Institute, Sydney, New South Wales, Australia.; Human Cancer and Viral Immunology Laboratory, The University of Sydney, Sydney, New South Wales, Australia., Wishart CL; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.; Viral immunopathology Laboratory, Infection, Immunity and Inflammation Research Theme, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia.; Ramaciotti Facility for Human System Biology, The University of Sydney and Centenary Institute, Sydney, New South Wales, Australia., Tan J; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia., Hsu P; Kids Research, The Children's Hospital at Westmead, Sydney, New South Wales, Australia.; Discipline of Child and Adolescent Health, The University of Sydney, Sydney, New South Wales, Australia., King NJC; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.; Sydney Cytometry Core Research Facility, Charles Perkins Centre, The University of Sydney and Centenary Institute, Sydney, New South Wales, Australia.; Viral immunopathology Laboratory, Infection, Immunity and Inflammation Research Theme, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia.; Ramaciotti Facility for Human System Biology, The University of Sydney and Centenary Institute, Sydney, New South Wales, Australia.; The University of Sydney Institute for Infectious Diseases, The University of Sydney, Sydney, New South Wales, Australia.; Sydney Nano, The University of Sydney, Sydney, New South Wales, Australia., Macia L; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.; Sydney Cytometry Core Research Facility, Charles Perkins Centre, The University of Sydney and Centenary Institute, Sydney, New South Wales, Australia.; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia., Nanan R; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.; Nepean Hospital, Nepean Blue Mountains Local Health District, Penrith, New South Wales, Australia.; Nepean Clinical School, The University of Sydney, Sydney, New South Wales, Australia.

Publikováno v: Immunology [Immunology] 2024 Sep; Vol. 173 (1), pp. 106-124. Date of Electronic Publication: 2024 May 26.

High-Dimensional Methods of Single-Cell Microglial Profiling to Enhance Understanding of Neuropathological Disease.

Autor: Spiteri AG; Viral Immunopathology Laboratory, Infection, Immunity and Inflammation Research Theme, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia.; Charles Perkins Centre, The University of Sydney, Sydney, Australia., Pilkington KR; Cytek Biosciences, Inc., Fremont, California., Wishart CL; Viral Immunopathology Laboratory, Infection, Immunity and Inflammation Research Theme, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia.; Charles Perkins Centre, The University of Sydney, Sydney, Australia., Macia L; Charles Perkins Centre, The University of Sydney, Sydney, Australia.; Sydney Cytometry, The University of Sydney and Centenary Institute, Sydney, Australia., King NJC; Viral Immunopathology Laboratory, Infection, Immunity and Inflammation Research Theme, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia.; Charles Perkins Centre, The University of Sydney, Sydney, Australia.; Sydney Cytometry, The University of Sydney and Centenary Institute, Sydney, Australia.; The University of Sydney Institute for Infectious Diseases, The University of Sydney, Sydney, Australia.; The University of Sydney Nano Institute, The University of Sydney, Sydney, Australia.

Publikováno v: Current protocols [Curr Protoc] 2024 Mar; Vol. 4 (3), pp. e985.

Integrating transcriptomic datasets across neurological disease identifies unique myeloid subpopulations driving disease-specific signatures.

Autor: Wishart CL; Infection, Immunity, Inflammation Research Theme, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia.; Sydney Cytometry Facility, The University of Sydney and Centenary Institute, Sydney, New South Wales, Australia.; Ramaciotti Facility for Human Systems Biology, The University of Sydney and Centenary Institute, Sydney, New South Wales, Australia.; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia., Spiteri AG; Infection, Immunity, Inflammation Research Theme, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia.; Sydney Cytometry Facility, The University of Sydney and Centenary Institute, Sydney, New South Wales, Australia.; Ramaciotti Facility for Human Systems Biology, The University of Sydney and Centenary Institute, Sydney, New South Wales, Australia.; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia., Locatelli G; Theodor Kocher Institute, University of Bern, Bern, Switzerland.; Novartis Institutes for BioMedical Research, Novartis, Basel, Switzerland., King NJC; Infection, Immunity, Inflammation Research Theme, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia.; Sydney Cytometry Facility, The University of Sydney and Centenary Institute, Sydney, New South Wales, Australia.; Ramaciotti Facility for Human Systems Biology, The University of Sydney and Centenary Institute, Sydney, New South Wales, Australia.; Charles Perkins Centre, The University of Sydney, Sydney, New South Wales, Australia.; Sydney Institute for Infectious Diseases, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia.; The University of Sydney Nano Institute, Faculty of Science, The University of Sydney, Sydney, New South Wales, Australia.

Publikováno v: Glia [Glia] 2023 Apr; Vol. 71 (4), pp. 904-925. Date of Electronic Publication: 2022 Dec 16.

High-parameter cytometry unmasks microglial cell spatio-temporal response kinetics in severe neuroinflammatory disease.

Autor: Spiteri AG; Discipline of Pathology, Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney, Sydney, Australia.; Charles Perkins Centre, The University of Sydney, Sydney, Australia., Terry RL; Discipline of Pathology, Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney, Sydney, Australia.; Charles Perkins Centre, The University of Sydney, Sydney, Australia.; Current Address: Children's Cancer Institute, Randwick, New South Wales, Australia.; Current Affiliation: Cancer Immunology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia., Wishart CL; Discipline of Pathology, Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney, Sydney, Australia.; Charles Perkins Centre, The University of Sydney, Sydney, Australia., Ashhurst TM; Discipline of Pathology, Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney, Sydney, Australia.; Charles Perkins Centre, The University of Sydney, Sydney, Australia.; Sydney Cytometry Facility, The University of Sydney and Centenary Institute, Sydney, Australia.; Ramaciotti Facility for Human Systems Biology, The University of Sydney and Centenary Institute, Sydney, Australia.; Marie Bashir Institute for Infectious Diseases and Biosecurity (MBI), Faculty of Medicine and Health, Sydney Medical School, The University of Sydney, Sydney, Australia., Campbell IL; Marie Bashir Institute for Infectious Diseases and Biosecurity (MBI), Faculty of Medicine and Health, Sydney Medical School, The University of Sydney, Sydney, Australia.; School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia., Hofer MJ; Charles Perkins Centre, The University of Sydney, Sydney, Australia.; Marie Bashir Institute for Infectious Diseases and Biosecurity (MBI), Faculty of Medicine and Health, Sydney Medical School, The University of Sydney, Sydney, Australia.; School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia., King NJC; Discipline of Pathology, Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney, Sydney, Australia. nicholas.king@sydney.edu.au.; Charles Perkins Centre, The University of Sydney, Sydney, Australia. nicholas.king@sydney.edu.au.; Sydney Cytometry Facility, The University of Sydney and Centenary Institute, Sydney, Australia. nicholas.king@sydney.edu.au.; Ramaciotti Facility for Human Systems Biology, The University of Sydney and Centenary Institute, Sydney, Australia. nicholas.king@sydney.edu.au.; Marie Bashir Institute for Infectious Diseases and Biosecurity (MBI), Faculty of Medicine and Health, Sydney Medical School, The University of Sydney, Sydney, Australia. nicholas.king@sydney.edu.au.; Nano Institute, The University of Sydney, Sydney, Australia. nicholas.king@sydney.edu.au.

Publikováno v: Journal of neuroinflammation [J Neuroinflammation] 2021 Jul 26; Vol. 18 (1), pp. 166. Date of Electronic Publication: 2021 Jul 26.