Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

Autor: Nadide Altincekic, Sophie Marianne Korn, Nusrat Shahin Qureshi, Marie Dujardin, Martí Ninot-Pedrosa, Rupert Abele, Marie Jose Abi Saad, Caterina Alfano, Fabio C. L. Almeida, Islam Alshamleh, Gisele Cardoso de Amorim, Thomas K. Anderson, Cristiane D. Anobom, Chelsea Anorma, Jasleen Kaur Bains, Adriaan Bax, Martin Blackledge, Julius Blechar, Anja Böckmann, Louis Brigandat, Anna Bula, Matthias Bütikofer, Aldo R. Camacho-Zarco, Teresa Carlomagno, Icaro Putinhon Caruso, Betül Ceylan, Apirat Chaikuad, Feixia Chu, Laura Cole, Marquise G. Crosby, Vanessa de Jesus, Karthikeyan Dhamotharan, Isabella C. Felli, Jan Ferner, Yanick Fleischmann, Marie-Laure Fogeron, Nikolaos K. Fourkiotis, Christin Fuks, Boris Fürtig, Angelo Gallo, Santosh L. Gande, Juan Atilio Gerez, Dhiman Ghosh, Francisco Gomes-Neto, Oksana Gorbatyuk, Serafima Guseva, Carolin Hacker, Sabine Häfner, Bing Hao, Bruno Hargittay, K. Henzler-Wildman, Jeffrey C. Hoch, Katharina F. Hohmann, Marie T. Hutchison, Kristaps Jaudzems, Katarina Jović, Janina Kaderli, Gints Kalniņš, Iveta Kaņepe, Robert N. Kirchdoerfer, John Kirkpatrick, Stefan Knapp, Robin Krishnathas, Felicitas Kutz, Susanne zur Lage, Roderick Lambertz, Andras Lang, Douglas Laurents, Lauriane Lecoq, Verena Linhard, Frank Löhr, Anas Malki, Luiza Mamigonian Bessa, Rachel W. Martin, Tobias Matzel, Damien Maurin, Seth W. McNutt, Nathane Cunha Mebus-Antunes, Beat H. Meier, Nathalie Meiser, Miguel Mompeán, Elisa Monaca, Roland Montserret, Laura Mariño Perez, Celine Moser, Claudia Muhle-Goll, Thais Cristtina Neves-Martins, Xiamonin Ni, Brenna Norton-Baker, Roberta Pierattelli, Letizia Pontoriero, Yulia Pustovalova, Oliver Ohlenschläger, Julien Orts, Andrea T. Da Poian, Dennis J. Pyper, Christian Richter, Roland Riek, Chad M. Rienstra, Angus Robertson, Anderson S. Pinheiro, Raffaele Sabbatella, Nicola Salvi, Krishna Saxena, Linda Schulte, Marco Schiavina, Harald Schwalbe, Mara Silber, Marcius da Silva Almeida, Marc A. Sprague-Piercy, Georgios A. Spyroulias, Sridhar Sreeramulu, Jan-Niklas Tants, Kaspars Tārs, Felix Torres, Sabrina Töws, Miguel Á. Treviño, Sven Trucks, Aikaterini C. Tsika, Krisztina Varga, Ying Wang, Marco E. Weber, Julia E. Weigand, Christoph Wiedemann, Julia Wirmer-Bartoschek, Maria Alexandra Wirtz Martin, Johannes Zehnder, Martin Hengesbach, Andreas Schlundt

Publikováno v: Frontiers in Molecular Biosciences, Vol 8 (2021)

The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium

Externí odkaz: https://doaj.org/article/201170fa84b8450e9b468d9fb4480774

Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications

Autor: Altincekic, Nadide, Korn, Sophie Marianne, Qureshi, Nusrat Shahin, Dujardin, Marie, Ninot-Pedrosa, Martí, Abele, Rupert, Abi Saad, Marie Jose, Alfano, Caterina, Almeida, Fabio, Alshamleh, Islam, de Amorim, Gisele Cardoso, Anderson, Thomas, Anobom, Cristiane, Anorma, Chelsea, Bains, Jasleen Kaur, Bax, Adriaan, Blackledge, Martin, Blechar, Julius, Böckmann, Anja, Brigandat, Louis, Bula, Anna, Bütikofer, Matthias, Camacho-Zarco, Aldo, Carlomagno, Teresa, Caruso, Icaro Putinhon, Ceylan, Betül, Chaikuad, Apirat, Chu, Feixia, Cole, Laura, Crosby, Marquise, de Jesus, Vanessa, Dhamotharan, Karthikeyan, Felli, Isabella, Ferner, Jan, Fleischmann, Yanick, Fogeron, Marie-Laure, Fourkiotis, Nikolaos, Fuks, Christin, Fürtig, Boris, Gallo, Angelo, Gande, Santosh, Gerez, Juan Atilio, Ghosh, Dhiman, GOMES-NETO, Francisco, Gorbatyuk, Oksana, Guseva, Serafima, Hacker, Carolin, Häfner, Sabine, Hao, Bing, Hargittay, Bruno, Henzler-Wildman, K., Hoch, Jeffrey, Hohmann, Katharina, Hutchison, Marie, Jaudzems, Kristaps, Jović, Katarina, Kaderli, Janina, Kalniņš, Gints, Kaņepe, Iveta, Kirchdoerfer, Robert, Kirkpatrick, John, Knapp, Stefan, Krishnathas, Robin, Kutz, Felicitas, zur Lage, Susanne, Lambertz, Roderick, Lang, Andras, Laurents, Douglas, Lecoq, Lauriane, Linhard, Verena, Löhr, Frank, Malki, Anas, Bessa, Luiza Mamigonian, Martin, Rachel, Matzel, Tobias, Maurin, Damien, McNutt, Seth, Mebus-Antunes, Nathane Cunha, Meier, Beat, Meiser, Nathalie, Mompeán, Miguel, Monaca, Elisa, Montserret, Roland, Mariño Perez, Laura, Moser, Celine, Muhle-Goll, Claudia, Neves-Martins, Thais Cristtina, Ni, Xiamonin, Norton-Baker, Brenna, Pierattelli, Roberta, Pontoriero, Letizia, Pustovalova, Yulia, Ohlenschläger, Oliver, Orts, Julien, Da Poian, Andrea, Pyper, Dennis, Richter, Christian, Riek, Roland, Rienstra, Chad, Robertson, Angus, Pinheiro, Anderson, Sabbatella, Raffaele, Salvi, Nicola, Saxena, Krishna, Schulte, Linda, Schiavina, Marco, Schwalbe, Harald, Silber, Mara, Almeida, Marcius da Silva, Sprague-Piercy, Marc, Spyroulias, Georgios, Sreeramulu, Sridhar, Tants, Jan-Niklas, Tārs, Kaspars, Torres, Felix, Töws, Sabrina, Treviño, Miguel, Trucks, Sven, Tsika, Aikaterini, Varga, Krisztina, Wang, Ying, Weber, Marco, Weigand, Julia, Wiedemann, Christoph, Wirmer-Bartoschek, Julia, Wirtz Martin, Maria Alexandra, Zehnder, Johannes, Hengesbach, Martin, Schlundt, Andreas, Treviño, Miguel Á.

Publikováno v: Frontiers in Molecular Biosciences
Frontiers in Molecular Biosciences, Frontiers Media, 2021, 8, pp.653148. ⟨10.3389/fmolb.2021.653148⟩
Frontiers in Molecular Biosciences, 2021, 8, pp.653148. ⟨10.3389/fmolb.2021.653148⟩
Digital.CSIC. Repositorio Institucional del CSIC
instname
Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
Frontiers in molecular biosciences, 8, Art.Nr. 653148
Frontiers in molecular biosciences
Switzerland
Digital.CSIC: Repositorio Institucional del CSIC
Consejo Superior de Investigaciones Científicas (CSIC)

The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0ea19ee1f73bbf80134d91f789c92dd
https://hal.univ-grenoble-alpes.fr/hal-03251639

1H, 13C and 15N chemical shift assignment of the stem-loops 5b + c from the 5′-UTR of SARS-CoV-2

Autor: Klara R. Mertinkus, J. Tassilo Grün, Nadide Altincekic, Jasleen Kaur Bains, Betül Ceylan, Jan-Peter Ferner, Lucio Frydman, Boris Fürtig, Martin Hengesbach, Katharina F. Hohmann, Daniel Hymon, Jihyun Kim, Božana Knezic, Mihajlo Novakovic, Andreas Oxenfarth, Stephen A. Peter, Nusrat S. Qureshi, Christian Richter, Tali Scherf, Andreas Schlundt, Robbin Schnieders, Harald Schwalbe, Elke Stirnal, Alexey Sudakov, Jennifer Vögele, Anna Wacker, Julia E. Weigand, Julia Wirmer-Bartoschek, Maria A. Wirtz Martin, Jens Wöhnert

Publikováno v: Biomolecular NMR Assignments, 16

The ongoing pandemic of the respiratory disease COVID-19 is caused by the SARS-CoV-2 (SCoV2) virus. SCoV2 is a member of the Betacoronavirus genus. The 30 kb positive sense, single stranded RNA genome of SCoV2 features 5'- and 3'-genomic ends that ar

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99f387aa7cf6d5f0b5a6907a6bb95fa8
https://hdl.handle.net/20.500.11850/534661

Exploring the druggability of conserved RNA regulatory elements in the SARS-CoV-2 genome

Autor: Sreeramulu, Sridhar, Richter, Christian, Berg, Hannes, Wirtz Martin, Maria A., Ceylan, Betül, Matzel, Tobias, Adam, Jennifer, Altincekic, Nadide, Azzaoui, Kamal, Bains, Jasleen Kaur, Blommers, Marcel Jules José, Ferner, Jan, Fürtig, Boris, Göbel, Michael, Grün, J. Tassilo, Hengesbach, Martin, Hohmann, Katharina F., Hymon, Daniel, Knezic, Bozana, Martins, Jason N., Mertinkus, Klara R., Niesteruk, Anna, Peter, Stephen A., Pyper, Dennis J., Qureshi, Nusrat, Scheffer, Ute, Schlundt, Andreas, Schnieders, Robbin, Stirnal, Elke, Sudakov, Alexey, Tröster, Alix Friederike, Vögele, Jennifer, Wacker, Anna, Weigand, Julia, Wirmer-Bartoschek, Julia, Wöhnert, Jens, Schwalbe, Harald

SARS-CoV-2 contains a positive single-stranded RNA genome of approximately 30 000 nucleotides. Within this genome, 15 RNA elements were identified as conserved between SARS-CoV and SARS-CoV-2. By nuclear magnetic resonance (NMR) spectroscopy, we prev

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od_______603::33ca31cb4fa187998c8715f91c92114b
http://publikationen.ub.uni-frankfurt.de/files/63928/container.zip

19F NMR-based fragment screening for 14 different biologically active RNAs and 10 DNA and protein counter-screens

Autor: Binas, Oliver, Jesus, Vanessa de, Landgraf, Tom, Völklein, Albrecht Eduard, Martins, Jason, Hymon, Daniel, Bains, Jasleen Kaur, Berg, Hannes, Biedenbänder, Thomas, Fürtig, Boris, Gande, Santosh Lakshmi, Niesteruk, Anna, Oxenfarth, Andreas, Qureshi, Nusrat, Schamber, Tatjana, Schnieders, Robbin, Tröster, Alix Friederike, Wacker, Anna, Wirmer-Bartoschek, Julia, Wirtz Martin, Maria Alexandra, Stirnal, Elke, Azzaoui, Kamal, Richter, Christian, Sreeramulu, Sridhar, Blommers, Marcel Jules José, Schwalbe, Harald

We report here the nuclear magnetic resonance 19F screening of 14 RNA targets with different secondary and tertiary structure to systematically assess the druggability of RNAs. Our RNA targets include representative bacterial riboswitches that natura

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od_______603::b89d87171f4abff9aa96cf3dee3e0936
http://publikationen.ub.uni-frankfurt.de/files/63851/container.zip