Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Winston X, Yan"'
Autor:
Colin McGaw, Anthony J. Garrity, Gabrielle Z. Munoz, Jeffrey R. Haswell, Sejuti Sengupta, Elise Keston-Smith, Pratyusha Hunnewell, Alexa Ornstein, Mishti Bose, Quinton Wessells, Noah Jakimo, Paul Yan, Huaibin Zhang, Lauren E. Alfonse, Roy Ziblat, Jason M. Carte, Wei-Cheng Lu, Derek Cerchione, Brendan Hilbert, Shanmugapriya Sothiselvam, Winston X. Yan, David R. Cheng, David A. Scott, Tia DiTommaso, Shaorong Chong
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-11 (2022)
Cas12i is a genome editing platform with compact size that fits in AAV vector with short 43-mer gRNA, absence of tracrRNA, ability to process pre-crRNA, and high specificity. Here the authors present an unbiased mutational scanning approach to engine
Externí odkaz:
https://doaj.org/article/b34236ad23cc4e1cb4a7c8a576cc399b
Autor:
Winston X. Yan, Reza Mirzazadeh, Silvano Garnerone, David Scott, Martin W. Schneider, Tomasz Kallas, Joaquin Custodio, Erik Wernersson, Yinqing Li, Linyi Gao, Yana Federova, Bernd Zetsche, Feng Zhang, Magda Bienko, Nicola Crosetto
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-9 (2017)
Double-strand breaks are a major DNA lesion that can occur by endogenous and exogenous processes. Here the authors present BLISS—Breaks LabellingIn Situand Sequencing—to map breaks across the genome.
Externí odkaz:
https://doaj.org/article/09a5cb9f162d419e8462fcfa6062aa33
Autor:
Annemieke, Aartsma-Rus, Alejandro, Garanto, Willeke, van Roon-Mom, Erin M, McConnell, Victoria, Suslovitch, Winston X, Yan, Jonathan K, Watts, Timothy W, Yu
Publikováno v:
Nucleic acid therapeutics.
Antisense oligonucleotides (ASOs) can modulate pre-mRNA splicing. This offers therapeutic opportunities for numerous genetic diseases, often in a mutation-specific and sometimes even individual-specific manner. Developing therapeutic ASOs for as few
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::4d19f24e2202c63305b6cb379df5b95b
https://pubmed.ncbi.nlm.nih.gov/36516128
https://pubmed.ncbi.nlm.nih.gov/36516128
Publikováno v:
PLoS Computational Biology, Vol 13, Iss 10, p e1005807 (2017)
The adaptation of the CRISPR-Cas9 system as a genome editing technique has generated much excitement in recent years owing to its ability to manipulate targeted genes and genomic regions that are complementary to a programmed single guide RNA (sgRNA)
Externí odkaz:
https://doaj.org/article/6589b9896e8c45599db19bc5212f70a6
Autor:
Philippe Horvath, Česlovas Venclovas, David R. Cheng, Feng Zhang, Daniel H. Haft, Sergey Shmakov, Yuri I. Wolf, Emmanuelle Charpentier, Stan J. J. Brouns, Sylvain Moineau, Winston X. Yan, Virginijus Siksnys, Malcolm F. White, Michael P. Terns, Francisco J. M. Mojica, Shiraz A. Shah, Omer S. Alkhnbashi, Eugene V. Koonin, Roger A. Garrett, Jaime Iranzo, David Scott, Alexander F. Yakunin, Rodolphe Barrangou, John van der Oost, Rolf Backofen, Kira S. Makarova
Publikováno v:
RUA. Repositorio Institucional de la Universidad de Alicante
Universidad de Alicante (UA)
Makarova, K S, Wolf, Y I, Iranzo, J, Shmakov, S A, Alkhnbashi, O S, Brouns, S J J, Charpentier, E, Cheng, D, Haft, D H, Horvath, P, Moineau, S, Mojica, F J M, Scott, D, Shah, S A, Siksnys, V, Terns, M P, Venclovas, Č, White, M F, Yakunin, A F, Yan, W, Zhang, F, Garrett, R A, Backofen, R, van der Oost, J, Barrangou, R & Koonin, E V 2020, ' Evolutionary classification of CRISPR-Cas systems : a burst of class 2 and derived variants ', Nature Reviews. Microbiology, vol. 18, pp. 67-83 . https://doi.org/10.1038/s41579-019-0299-x
Nat Rev Microbiol
Nature Reviews Microbiology, 18, 67-83
Nature Reviews Microbiology 18 (2020)
Universidad de Alicante (UA)
Makarova, K S, Wolf, Y I, Iranzo, J, Shmakov, S A, Alkhnbashi, O S, Brouns, S J J, Charpentier, E, Cheng, D, Haft, D H, Horvath, P, Moineau, S, Mojica, F J M, Scott, D, Shah, S A, Siksnys, V, Terns, M P, Venclovas, Č, White, M F, Yakunin, A F, Yan, W, Zhang, F, Garrett, R A, Backofen, R, van der Oost, J, Barrangou, R & Koonin, E V 2020, ' Evolutionary classification of CRISPR-Cas systems : a burst of class 2 and derived variants ', Nature Reviews. Microbiology, vol. 18, pp. 67-83 . https://doi.org/10.1038/s41579-019-0299-x
Nat Rev Microbiol
Nature Reviews Microbiology, 18, 67-83
Nature Reviews Microbiology 18 (2020)
The number and diversity of known CRISPR–Cas systems have substantially increased in recent years. Here, we provide an updated evolutionary classification of CRISPR–Cas systems and cas genes, with an emphasis on the major developments that have o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cce78d031229e83206ad0cdc202de35f
https://doi.org/10.1038/s41579-019-0299-x
https://doi.org/10.1038/s41579-019-0299-x
Autor:
Wen Y. Wu, Prarthana Mohanraju, Chunyu Liao, Belén Adiego-Pérez, Sjoerd C.A. Creutzburg, Kira S. Makarova, Karlijn Keessen, Timon A. Lindeboom, Tahseen S. Khan, Stijn Prinsen, Rob Joosten, Winston X. Yan, Anzhela Migur, Charlie Laffeber, David A. Scott, Joyce H.G. Lebbink, Eugene V. Koonin, Chase L. Beisel, John van der Oost
Publikováno v:
Molecular Cell, 82(23), 4487-4502.e7. Cell Press
Molecular Cell 82 (2022) 23
Molecular Cell, 82(23), 4487-4502.e7
Molecular Cell 82 (2022) 23
Molecular Cell, 82(23), 4487-4502.e7
CRISPR-Cas are prokaryotic adaptive immune systems. Cas nucleases generally use CRISPR-derived RNA guides to specifically bind and cleave DNA or RNA targets. Here, we describe the experimental characterization of a bacterial CRISPR effector protein C
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e31cb63451bcbb54c861a5935573b7b
https://doi.org/10.1016/j.molcel.2022.11.003
https://doi.org/10.1016/j.molcel.2022.11.003
Autor:
Guilhem Faure, Eugene V. Koonin, David Scott, David R. Cheng, Kira S. Makarova, Sergey Shmakov, Joseph E. Peters, Winston X. Yan
Publikováno v:
Nature Reviews Microbiology. 17:513-525
The principal function of CRISPR–Cas systems in archaea and bacteria is defence against mobile genetic elements (MGEs), including viruses, plasmids and transposons. However, the relationships between CRISPR–Cas and MGEs are far more complex. Seve
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f6a8fcfa428502e79c5daa136f79c98a
https://doi.org/10.1038/s41579-019-0204-7
https://doi.org/10.1038/s41579-019-0204-7
Autor:
Takashi Yamano, Linyi Gao, Winston X. Yan, Osamu Nureki, Nicola Crosetto, Feng Zhang, Martin W. Schneider, David Benjamin Turitz Cox, Hiroshi Nishimasu, John C. Manteiga
Publikováno v:
Nature Biotechnology. 35:789-792
The RNA-guided endonuclease Cpf1 is a promising tool for genome editing in eukaryotic cells. However, the utility of the commonly used Acidaminococcus sp. BV3L6 Cpf1 (AsCpf1) and Lachnospiraceae bacterium ND2006 Cpf1 (LbCpf1) is limited by their requ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::09584ecdd97276c972ffc989b48c5da8
https://doi.org/10.1038/nbt.3900
https://doi.org/10.1038/nbt.3900
Autor:
David Benjamin Turitz Cox, Kira S. Makarova, Feng Zhang, Aaron Smargon, David A. Scott, Jonathan S. Gootenberg, Omar O. Abudayyeh, Yuri I. Wolf, Eugene V. Koonin, Konstantin Severinov, Neena Pyzocha, Sergey Shmakov, Winston X. Yan
Publikováno v:
PMC
Class 2 CRISPR-Cas systems are characterized by effector modules that consist of a single multidomain protein, such as Cas9 or Cpf1. We designed a computational pipeline for the discovery of novel class 2 variants and used it to identify six new CRIS
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc7588e000f82b8630be9362c907cfd9
https://doi.org/10.1038/nrmicro.2016.184
https://doi.org/10.1038/nrmicro.2016.184
Autor:
Lauren E. Alfonse, David R. Cheng, Elise Keston-Smith, Anthony J. Garrity, Jason M. Carte, Eugene V. Koonin, Pratyusha Hunnewell, Shaorong Chong, David Scott, Shanmugapriya Sothiselvam, Kira S. Makarova, Winston X. Yan
Publikováno v:
Science (New York, N.Y.). 363(6422)
Additional, diverse CRISPR systems CRISPR systems have been revolutionizing molecular biology. Mining the metagenomic database, Yan et al. systematically discovered additional subtypes of type V CRISPR-Cas systems. The additional Cas12 effectors disp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::75481061a93c976c932d3ab9a064e070
https://pubmed.ncbi.nlm.nih.gov/31021231
https://pubmed.ncbi.nlm.nih.gov/31021231