Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Wim Pomp"'
Autor:
Ineke Brouwer, Heta Piyush Patel, Joseph Victor Willem Meeussen, Wim Pomp, Tineke Laura Lenstra
Publikováno v:
STAR Protocols, Vol 1, Iss 3, Pp 100142- (2020)
Summary: This protocol describes how to image fluorescently tagged proteins, RNA, or DNA inside living Saccharomyces cerevisiae cells at the single-molecule level. Imaging inside living cells, as opposed to fixed materials, gives access to real-time
Externí odkaz:
https://doaj.org/article/978925e111a44a238ec5f246a4609285
Single-molecule imaging inside living cells has revealed that transcription factors (TFs) bind to DNA transiently, but a long-standing question is how this transient binding is related to transcription activation. Here, we devised a microscopy method
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::975d3dcd2e6ab797062003a229260ee7
https://doi.org/10.1101/2023.05.15.540758
https://doi.org/10.1101/2023.05.15.540758
Autor:
Joseph V.W. Meeussen, Wim Pomp, Ineke Brouwer, Wim J. de Jonge, Heta P. Patel, Tineke L. Lenstra
Many transcription factors (TFs) localize in nuclear clusters of locally increased concentrations, but how TF clustering is regulated and how it influences gene expression is not well understood. Here, we use quantitative microscopy in living cells t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23882642f4bfb58d5c94321ba910b1f4
https://doi.org/10.1101/2022.12.13.520200
https://doi.org/10.1101/2022.12.13.520200
Autor:
Heta P. Patel, Stefano Coppola, Wim Pomp, Umberto Aiello, Ineke Brouwer, Domenico Libri, Tineke L. Lenstra
Publikováno v:
Molecular Cell. 83:1573-1587.e8
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6361d1f829ba48f21aac64adbbb7fd53
https://doi.org/10.1016/j.molcel.2023.04.015
https://doi.org/10.1016/j.molcel.2023.04.015
DNA supercoiling has emerged as a major contributor to gene regulation in bacteria. The impact of DNA supercoiling on transcription dynamics in eukaryotes is less clear. Here, using single-molecule dual-color RNA imaging in budding yeast, we show tha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::46f9f6376208fcefee6f5ba8b7fa83e6
https://doi.org/10.1101/2022.03.04.482969
https://doi.org/10.1101/2022.03.04.482969
Autor:
Luca Giomi, Jeremy Ernst, Thomas Schmidt, Wim Pomp, Erik H.J. Danen, Roeland M. H. Merks, Koen Schakenraad
Publikováno v:
Soft Matter, 16(27), 6328-6343
We investigate the mechanical interplay between the spatial organization of the actin cytoskeleton and the shape of animal cells adhering on micropillar arrays. Using a combination of analytical work, computer simulations and in vitro experiments, we
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f72faa29b393b73534ff4db16d179b3
https://pubmed.ncbi.nlm.nih.gov/32490503
https://pubmed.ncbi.nlm.nih.gov/32490503
Autor:
David Peale, Gleb Shtengel, David J. Solecki, Jennifer Lippincott-Schwartz, Daniel R. Stabley, Tom Kirchhausen, Harald F. Hess, Nirmala Iyer, Song Pang, David P. Hoffman, Kathy Schaefer, Melanie Freeman, Daniel E. Milkie, Lei Wang, Eric Betzig, H. Amalia Pasolli, Abbas Shirinifard, Chi-Lun Chang, Kirby R. Campbell, John A. Bogovic, Wim Pomp, C. Shan Xu
Publikováno v:
Science
Visualizing whole cells at many scales Cells need to compartmentalize thousands of distinct proteins, but the nanoscale spatial relationship of many proteins to overall intracellular ultrastructure remains poorly understood. Correlated light and elec
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4020d4606b2e1a7dbdff319dc7424a2
Autor:
Hedde van Hoorn, Wim Pomp, Luca Giomi, Hayri E. Balcioglu, Erik H.J. Danen, Roeland M. H. Merks, Koen Schakenraad, Thomas Schmidt
Publikováno v:
Physical Review Letters
We investigate the geometrical and mechanical properties of adherent cells characterized by a highly anisotropic actin cytoskeleton. Using a combination of theoretical work and experiments on micropillar arrays, we demonstrate that the shape of the c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b636c8f30acc3d79328b5d46808320c1
http://arxiv.org/abs/1702.03916
http://arxiv.org/abs/1702.03916
Autor:
Sven H. C. Askes, Hadi Arjmandi-Tash, Thomas Schmidt, Vincent Christiaan Leeuwenburgh, Wim Pomp, Sylvestre Bonnet, Stefania Tanase
Publikováno v:
ACS Biomaterials Science & Engineering
ACS Biomaterials Science and Engineering, 3(3), 322-334
ACS Biomaterials Science and Engineering
ACS Biomaterials Science and Engineering, 3(3), 322-334
ACS Biomaterials Science and Engineering
Light upconversion by triplet-triplet annihilation (TTA-UC) in nanoparticles has received considerable attention for bioimaging and light activation of prodrugs. However, the mechanism of TTA-UC is inherently sensitive for quenching by molecular oxyg
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::620f41434db67d77bea8ea8af0c20ec4
https://hdl.handle.net/1887/58227
https://hdl.handle.net/1887/58227
Autor:
Samantha L. Hopkins, Sven H. C. Askes, Alexander Kros, Thomas Schmidt, Si Wu, Wim Pomp, Sylvestre Bonnet
Publikováno v:
Small, 12(40), 5579-5590
Small
Small
Light upconversion is a very powerful tool in bioimaging as it can eliminate autofluorescence, increase imaging contrast, reduce irradiation damage, and increase excitation penetration depth in vivo. In particular, triplet-triplet annihilation upconv
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aea972c7b6b81e029f90e86c16f79058
https://pubmed.ncbi.nlm.nih.gov/27571308
https://pubmed.ncbi.nlm.nih.gov/27571308