Zobrazeno 1 - 10
of 45
pro vyhledávání: '"Willy S. Bont"'
Publikováno v:
International Journal of Protein Research. 1:193-197
The incorporation of amino acids into TCA * precipitable polypeptides has been studied in a cell-free system isolated from rat liver. A new procedure is described for the isolation of all amino-acyl-tRNA synthetases necessary for optimal amino acid i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b6af7ccc2778a7d6a8c5681f8cd4b73
https://doi.org/10.1111/j.1399-3011.1969.tb01643.x
https://doi.org/10.1111/j.1399-3011.1969.tb01643.x
Autor:
Willy S. Bont
Publikováno v:
Molecular Biology Reports. 36:959-964
In order to explain the experimentally obtained results with biomembranes it has been assumed, that the diameter of all vesicles, defined by a lipid bilayer, form a perfect crystal. Later on it appeared that biomembranes are liquid crystals. Neverthe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fbc99923f79a897f0d651af9c8701e58
https://doi.org/10.1007/s11033-008-9268-6
https://doi.org/10.1007/s11033-008-9268-6
Publikováno v:
Journal of Immunological Methods. 29:1-16
An improved method of separating human peripheral blood lymphocytes and monocytes by velocity sedimentation at unit gravity is described. Cell separation capacity is considerably increased, while the time required for optimal separation is markedly r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba396ec9f749e91546587fc7c435a632
https://doi.org/10.1016/0022-1759(79)90120-0
https://doi.org/10.1016/0022-1759(79)90120-0
Publikováno v:
International Journal of Cancer. 25:73-84
Lymphocytes from 12 healthy donors were depleted of monocytes by velocity sedimentation at unit gravity (average monocyte contamination 0.2%) and tested for spontaneous cytotoxicity (SC) against tumor target cells in 24 h microcytotoxicity assays. Si
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::516cc7ec8a954ca0e161bba23d12c54f
https://doi.org/10.1002/ijc.2910250110
https://doi.org/10.1002/ijc.2910250110
Publikováno v:
Blood. 60:46-53
Human peripheral blood monocytes were isolated by counterflow centrifugation elutriation (CCE). This technique was modified in such a way that various monocyte fractions (viability greater than 99%) could be elutriated by increasing the density of th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ac4b488474f3e07a62c4f8157344d869
https://doi.org/10.1182/blood.v60.1.46.46
https://doi.org/10.1182/blood.v60.1.46.46
Publikováno v:
Molecular Biology Reports. 2:379-384
Aminoacyl-tRNA synthetases from rat-liver cytoplasm were fractionated into two groups, characterized by their sedimentation coefficients of about 20S and 5S, respectively. These two groups of synthetases could be isolated from postmicrosomal supernat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a29b22c9186a4df0bf8cdb7ec26ae87
https://doi.org/10.1007/bf00366259
https://doi.org/10.1007/bf00366259
Autor:
Willy S. Bont
Publikováno v:
Journal of Theoretical Biology. 112:505-512
Vesicles formed by the fragmentation of biomembranes form discrete size classes. Their surface areas can be represented by two geometric series with a common ratio of 2. The surface areas of membrane vesicles found in intact cells, including nuclear
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a696df8551f084aea47cce61ef48db49
https://doi.org/10.1016/s0022-5193(85)80018-7
https://doi.org/10.1016/s0022-5193(85)80018-7
Autor:
W.L. van Es, Willy S. Bont
Publikováno v:
Analytical Biochemistry. 103:295-301
Minor alterations of the electrical circuitry of the centrifuge required for centrifugal elutriation are described. Furthermore it is shown that elutriation can be performed by hydrostatic pressure without the utilization of a pump. This approach ena
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85051a8bacef35dc4bace435f3b75f90
https://doi.org/10.1016/0003-2697(80)90613-2
https://doi.org/10.1016/0003-2697(80)90613-2
Autor:
Willy S. Bont
Publikováno v:
Journal of Theoretical Biology. 96:707-722
Vesicular fragments of biomembranes either induced or present as vesicles inside cells, have diameters which fall into two geometric series. Cells with intact cytoskeletons can take up a range of sizes but at mitosis, the cytoskeleton disappears, the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ab6e0dec44c65c3f4aa4cf06cfa79dd
https://doi.org/10.1016/0022-5193(82)90238-7
https://doi.org/10.1016/0022-5193(82)90238-7
Publikováno v:
The Journal of Immunology. 122:1099-1107
Studies were performed to determine the role of monocytes in human lymphocyte activation by mitogens. Velocity sedimentation at 1 × G in a new apparatus was utilized to obtain highly purified lymphocyte fractions (LF) nearly free of monocytes (0.02
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c2fdeb052bd515e03665755f2d38282d
https://doi.org/10.4049/jimmunol.122.3.1099
https://doi.org/10.4049/jimmunol.122.3.1099