Zobrazeno 1 - 10
of 23
pro vyhledávání: '"William W Wilfinger"'
Publikováno v:
PLoS ONE, Vol 18, Iss 11, p e0291209 (2023)
Numerous methodologies are used for blood RNA extraction, and large quantitative differences in recovered RNA content are reported. We evaluated three archived data sets to determine how extraction methodologies might influence mRNA and lncRNA sequen
Externí odkaz:
https://doaj.org/article/e8dda4681ce241eba309e59d21c63d60
Autor:
Piotr Chomczynski, Peter W Chomczynski, Amy Kennedy, Michal Rymaszewski, William W Wilfinger, Judith A Heiny, Karol Mackey
Publikováno v:
PLoS ONE, Vol 16, Iss 2, p e0246867 (2021)
Widespread diagnostic testing is needed to reduce transmission of COVID-19 and manage the pandemic. Effective mass screening requires robust and sensitive tests that reliably detect the SARS-CoV-2 virus, including asymptomatic and pre-symptomatic inf
Externí odkaz:
https://doaj.org/article/fefc16edc817473fa221cd4e7ad7984b
Autor:
Piotr Chomczynski, William W Wilfinger, Hamid R Eghbalnia, Amy Kennedy, Michal Rymaszewski, Karol Mackey
Publikováno v:
PLoS ONE, Vol 11, Iss 2, p e0148260 (2016)
Relatively little is known about the range of RNA levels in human blood. This report provides assessment of peripheral blood RNA level and its inter-individual differences in a group of 35 healthy humans consisting of 25 females and 10 males ranging
Externí odkaz:
https://doaj.org/article/e6202ed379944425a5004bd33268fd15
Autor:
Karol Mackey, Robert N.G. Miller, Hamid R. Eghbalnia, William W. Wilfinger, Piotr Chomczynski
Publikováno v:
BMC Genomics
BMC Genomics, Vol 22, Iss 1, Pp 1-19 (2021)
BMC Genomics, Vol 22, Iss 1, Pp 1-19 (2021)
Background RNA sequencing analysis focus on the detection of differential gene expression changes that meet a two-fold minimum change between groups. The variability present in RNA sequencing data may obscure the detection of valuable information whe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4bb331393d3099d311cdbf49b66ffe7f
https://pubmed.ncbi.nlm.nih.gov/33941086
https://pubmed.ncbi.nlm.nih.gov/33941086
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
Scientific Reports
Scientific Reports
RNA-Seq expression analysis currently relies primarily upon exon expression data. The recognized role of introns during translation, and the presence of substantial RNA-Seq counts attributable to introns, provide the rationale for the simultaneous co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::889c6a9bae56873b2bed33f4ecb8e679
https://doi.org/10.1038/s41598-020-72482-w
https://doi.org/10.1038/s41598-020-72482-w
Publikováno v:
eLS
The original single-step method is the first procedure to isolate purified total ribonucleic acid (RNA) from a variety of sources including tissues and cells from human, animal, plant, yeast, bacterial and viral origins, without the requirement of hi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc2d017965accd4bd94766778b6c46f3
https://doi.org/10.1002/9780470015902.a0003799.pub3
https://doi.org/10.1002/9780470015902.a0003799.pub3
Autor:
Hamid R. Eghbalnia, Piotr Chomczynski, Karol Mackey, Michal Rymaszewski, William W. Wilfinger, Amy Kennedy
Publikováno v:
PLoS ONE, Vol 11, Iss 2, p e0148260 (2016)
PLoS ONE
PLoS ONE
Relatively little is known about the range of RNA levels in human blood. This report provides assessment of peripheral blood RNA level and its inter-individual differences in a group of 35 healthy humans consisting of 25 females and 10 males ranging
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13f7bc4d0745aafcfd7a74e67bb9d5be
http://europepmc.org/articles/PMC4749217?pdf=render
http://europepmc.org/articles/PMC4749217?pdf=render
Publikováno v:
BioTechniques. 22:550-553
In this report, we present DNAzol®, a patent-pending DNA isolation reagent containing guanidine thiocyanate and a detergent mixture. It is a complete, nontoxic and ready-to-use reagent for the isolation of genomic DNA from various biological sources
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef175325764ab03a362f7b4f6d5f5b44
https://doi.org/10.2144/97223pf01
https://doi.org/10.2144/97223pf01
Publikováno v:
BioTechniques. 22:474-481
The ratio of absorbance at 260 and 280 nm (the A260/280 ratio) is frequently used to assess the purity of RNA and DNA preparations. Data presented in this report demonstrate significant variability in the RNA A260/280 ratio when different sources of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3833307a36961aacc3bd61a0a78b7e54
https://doi.org/10.2144/97223st01
https://doi.org/10.2144/97223st01
Autor:
Lin Chen, William W. Wilfinger, Carol Skowron Lomneth, William J. Larsen, E. Michael Hendrix, Simon J.T. Mao, Elliot L. Hertzberg
Publikováno v:
Tissue and Cell. 24:61-73
A radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) were used to determine relative concentrations of liver connexin32 (CX32) in rats. The RIA and ELISA utilize synthetic peptides corresponding to regions of the carboxyl-terminus a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e53fdd6d1e39ca89b53644b2d6b43303
https://doi.org/10.1016/0040-8166(92)90081-h
https://doi.org/10.1016/0040-8166(92)90081-h