Zobrazeno 1 - 10
of 29
pro vyhledávání: '"William Schook"'
Publikováno v:
Journal of neurochemistry. 54(1)
The 50-kilodalton (kDa) assembly polypeptide of bovine brain clathrin coated vesicles (CCVs) is phosphorylated in a cyclic nucleotide- and Ca2+-independent manner and is dephosphorylated by a Mg2+-ATP-dependent CCV phosphatase. This report provides e
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 598:447-455
Clathrin was isolated in highly purified form from bovine brain preparations rich in coated vesicles and by some improvements of our previous procedures. At pH 7.5, clathrin's solution was viscous, but clear. At pH 6.5, clathrin's solution was less v
Publikováno v:
Biochemical and Biophysical Research Communications. 115:94-99
Phospholipase A2 in the presence of Ca2+ was stimulated by calmodulin and by prostaglandin F2α. Prostaglandin E2, cyclic-AMP and cyclic-GMP inhibited phospholipase A2 in the presence or absence of calmodulin. Dimethylsuberimidate cross-linking of ph
Autor:
William Schook, Elena Puszkin, Saul Puszkin, Richard E. Rosenfield, Christine Ores, Charles Rouault, Jonathan Maimon, Shaul Kochwa
Publikováno v:
Journal of Biological Chemistry. 252:5529-5537
alpha-Actinin isolated from dog muscle was used to incite antibodies in rabbits, Antibodies, purified by affinity chromatography on CNBr-Sepharose coupled with alpha-actinin and then ferritin-labeled were found to localize on the Z disc of muscle sar
Publikováno v:
Journal of Neurochemistry. 38:1742-1747
A systematic characterization of the affinity of calmodulin for brain coated vesicles was undertaken. Binding of 125I-labeled calmodulin to coated vesicles was saturable and competed with unlabeled calmodulin, but not with troponin-C. Scatchard analy
Autor:
Lawrence Shapiro, Saul Puszkin, Michael P. Lisanti, Elaine L. Hua, William Schook, Nathan Moskowitz
Publikováno v:
European Journal of Biochemistry. 125:463-470
Clathrin-associated proteins were separated from clathrin under various clathrin-denaturing conditions, i.e. heating, freezing and isoelectric precipitation. The proteins retained biological activity; they were purified further by affinity chromatogr
Publikováno v:
FEBS Letters. 164:303-306
Clathrin-associated proteins purifies from bovine brain exhibited an ultraviolet spectrum with absorbance maximum at 256 nm and were found to contain tightly bound nucleotide. This nucleotide was identified as AMP and/or ADP by thin-layer and high-pe
Publikováno v:
Journal of Neurochemistry. 41:1576-1586
Brain synaptic vesicle phospholipase A2 (PLA2) activity was characterized. It is Ca2+-dependent and has a pH optimum of 9.0. The enzyme has a Km of 60 microM and a Vmax of 2.0 nmol/mg/h. Calmodulin, prostaglandin F2 alpha, and cAMP, and ATP all incre
Autor:
Nathan Moskowitz, Kenan Haver, E L Hua, William Schook, Michael P. Lisanti, W S Bloom, Saul Puszkin
Publikováno v:
Journal of Histochemistry & Cytochemistry. 30:497-503
The interaction of clathrin with cytoskeletal proteins was studied cytochemically by immunofluorescent staining and biochemically by the binding of actin to clathrin on the surfaces of polystyrene particles. Using a cytoskeletal-disrupting agent, the
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure. 494:144-149
Immunological identification of an antigen resolved from a protein complex by sodium dodecyl sulfate polyacrylamide gel electrophoresis has been attained. The identification is based on the formation of immunoprecipitin lines after the antigen diffus