Zobrazeno 1 - 10
of 27
pro vyhledávání: '"William Rostain"'
Autor:
Cyril Malbranke, William Rostain, Florence Depardieu, Simona Cocco, Rémi Monasson, David Bikard
Publikováno v:
PLoS Computational Biology, Vol 19, Iss 11, p e1011621 (2023)
We present here an approach to protein design that combines (i) scarce functional information such as experimental data (ii) evolutionary information learned from a natural sequence variants and (iii) physics-grounded modeling. Using a Restricted Bol
Externí odkaz:
https://doaj.org/article/05b943bf0d4e4bd9be8e836d8265bc37
Autor:
William Rostain, Tom Zaplana, Magali Boutard, Chloé Baum, Sibylle Tabuteau, Mary Sanitha, Mohandass Ramya, Adam Guss, Laurence Ettwiller, Andrew C. Tolonen
Publikováno v:
ACS Synthetic Biology
ACS Synthetic Biology, 2022, 11 (12), pp.4077-4088. ⟨10.1021/acssynbio.2c00385⟩
ACS Synthetic Biology, 2022, 11 (12), pp.4077-4088. ⟨10.1021/acssynbio.2c00385⟩
International audience
Publikováno v:
BioDesign Research, Vol 2020 (2020)
RNAs of different shapes and sizes, natural or synthetic, can regulate gene expression in prokaryotes and eukaryotes. Circular RNAs have recently appeared to be more widespread than previously thought, but their role in prokaryotes remains elusive. H
Externí odkaz:
https://doaj.org/article/3419720c30f04fcfbf4ce4dfac71658a
Autor:
Tristan Cerisy, William Rostain, Audam Chhun, Magali Boutard, Marcel Salanoubat, Andrew C. Tolonen
Publikováno v:
mSphere, Vol 4, Iss 6 (2019)
ABSTRACT Clostridia are a group of Gram-positive anaerobic bacteria of medical and industrial importance for which limited genetic methods are available. Here, we demonstrate an approach to make large genomic deletions and insertions in the model Clo
Externí odkaz:
https://doaj.org/article/ab05c80fb10a4397b257fe22ee7054a1
Autor:
William Rostain, Theophile Grebert, Danylo Vyhovskyi, Paula Thiel Pizarro, Gatwa Tshinsele-Van Bellingen, Lun Cui, David Bikard
Publikováno v:
Nucleic Acids Research
Nucleic Acids Research, 2023, 51 (7), pp.3485-3496. ⟨10.1093/nar/gkad170⟩
Nucleic Acids Research, 2023, 51 (7), pp.3485-3496. ⟨10.1093/nar/gkad170⟩
Genetic tools derived from the Cas9 RNA-guided nuclease are providing essential capabilities to study and engineer bacteria. While the importance of off-target effects was noted early in Cas9’s application to mammalian cells, off-target cleavage by
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d5a2a90b6a567fdcddb4f2416aa3ddf
https://hal-pasteur.archives-ouvertes.fr/pasteur-04130634/document
https://hal-pasteur.archives-ouvertes.fr/pasteur-04130634/document
Publikováno v:
Nucleic acids research
United States
England
Nucleic Acids Research
United States
England
Nucleic Acids Research
CRISPR technologies increasingly require spatiotemporal and dosage control of nuclease activity. One promising strategy involves linking nuclease activity to a cell's transcriptional state by engineering guide RNAs (gRNAs) to function only after comp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fafe7eb4469ab5fcbca775d23fee9e6
https://hdl.handle.net/10033/622791
https://hdl.handle.net/10033/622791
Autor:
Charlotte M Quinn, John N. Duncan, Marko Storch, William Rostain, Roberto Galizi, Manish Kushwaha, Alfonso Jaramillo
Publikováno v:
The Crispr Journal
The Crispr Journal, 2020, 3 (5), pp.398-408. ⟨10.1089/crispr.2020.0029⟩
The CRISPR Journal
The CRISPR Journal, 2020, 3 (5), pp.398-408. ⟨10.1089/crispr.2020.0029⟩
The Crispr Journal, 2020, 3 (5), pp.398-408. ⟨10.1089/crispr.2020.0029⟩
The CRISPR Journal
The CRISPR Journal, 2020, 3 (5), pp.398-408. ⟨10.1089/crispr.2020.0029⟩
International audience; CRISPR guide RNAs (gRNAs) can be programmed with relative ease to allow the genetic editing of nearly any DNA or RNA sequence. Here, we propose novel molecular architectures to achieve RNA-dependent modulation of CRISPR activi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b244e9cea2e44e375791ed09c88f7030
Publikováno v:
BioDesign Research, Vol 2020 (2020)
RNAs of different shapes and sizes, natural or synthetic, can regulate gene expression in prokaryotes and eukaryotes. Circular RNAs have recently appeared to be more widespread than previously thought, but their role in prokaryotes remains elusive. H
Autor:
Audam Chhun, Tristan Cerisy, Andrew C. Tolonen, Marcel Salanoubat, Magali Boutard, William Rostain
Publikováno v:
MSphere
MSphere, 2019, ⟨10.1128/mSphere⟩
mSphere, Vol 4, Iss 6 (2019)
mSphere, Vol 4, Iss 6, p e00710-19 (2019)
MSphere, American Society for Microbiology., 2019, ⟨10.1128/mSphere⟩
MSphere, 2019, 4 (6), ⟨10.1128/mSphere.00710-19⟩
mSphere
MSphere, American Society for Microbiology., 2019, 4 (6), ⟨10.1128/mSphere.00710-19⟩
MSphere, 2019, ⟨10.1128/mSphere⟩
mSphere, Vol 4, Iss 6 (2019)
mSphere, Vol 4, Iss 6, p e00710-19 (2019)
MSphere, American Society for Microbiology., 2019, ⟨10.1128/mSphere⟩
MSphere, 2019, 4 (6), ⟨10.1128/mSphere.00710-19⟩
mSphere
MSphere, American Society for Microbiology., 2019, 4 (6), ⟨10.1128/mSphere.00710-19⟩
Clostridia are anaerobic bacteria with important roles in intestinal and soil microbiomes. The inability to experimentally modify the genomes of clostridia has limited their study and application in biotechnology. Here, we developed a targetron-recom
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce72f433b4086e3b3896a92ebf75f858
https://hal.science/hal-03000600/document
https://hal.science/hal-03000600/document
Autor:
Magali Boutard, William Rostain, Alain Perret, Marcel Salanoubat, Christine Pelle, Andrew C. Tolonen, Tristan Cerisy, Henri-Pierre Fierobe, Alba Iglesias
Publikováno v:
Journal of Bacteriology
Journal of Bacteriology, American Society for Microbiology, 2019, 201 (15), ⟨10.1128/JB.00241-19⟩
Journal of Bacteriology, 2019, 201 (15), ⟨10.1128/JB.00241-19⟩
Journal of Bacteriology, American Society for Microbiology, 2019, 201 (15), ⟨10.1128/JB.00241-19⟩
Journal of Bacteriology, 2019, 201 (15), ⟨10.1128/JB.00241-19⟩
The mechanisms by which bacteria uptake solutes across the cell membrane broadly impact their cellular energetics. Here, we use functional genomic, genetic, and biophysical approaches to reveal how Clostridium (Lachnoclostridium) phytofermentans, a m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73c57bfb22be7215e573ab18d85b6685
https://hal.archives-ouvertes.fr/hal-02329534
https://hal.archives-ouvertes.fr/hal-02329534