Zobrazeno 1 - 10
of 35
pro vyhledávání: '"William R. Kenealy"'
Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood
Autor:
Sean F. Covalla, Steven D. Brown, Vasiliki Tsakraklides, William R. Kenealy, Indraneel Shikhare, David A. Hogsett, Lee R. Lynd, Abigail S. Foster, John S. Bardsley, Daniel G. Olson, W. Ryan Sillers, A. Joe Shaw, Christopher D. Herring, Philip G. Thorne, Jessica P. Johnson, Brian H. Davison, Jiayi Zhang, Dawn M. Klingeman, Stephen R. Rogers
Publikováno v:
Biotechnology for Biofuels
Background The thermophilic, anaerobic bacterium Thermoanaerobacterium saccharolyticum digests hemicellulose and utilizes the major sugars present in biomass. It was previously engineered to produce ethanol at yields equivalent to yeast. While saccha
Publikováno v:
Journal of Industrial Microbiology & Biotechnology. 38:1649-1655
Corn stover that had been treated with vapor-phase diethyl oxalate released a mixture of mono- and oligosaccharides consisting mainly of xylose and glucose. Following overliming and neutralization, a D-xylulokinase mutant of Pichia stipitis, FPL-YS30
Publikováno v:
Biomass and Bioenergy. 35:626-636
Autohydrolysis is a simple, green method of recovering sugars from biomass, using only hot water. One potential drawback is that byproducts are formed during the autohydrolysis process that could interfere with subsequent hydrolysis and fermentation
Publikováno v:
International Biodeterioration & Biodegradation. 62:372-375
Oxalate from partially decayed southern pine wood was analyzed by HPLC or colorimetric assay. Oxalate extraction efficiency, assessed by comparing analysis of whole wood cubes with ground wood, showed that both wood geometries could be extracted with
Publikováno v:
Applied Microbiology and Biotechnology. 66:635-640
Fermentation residues (consisting of incompletely fermented fiber, adherent bacterial cells, and a glycocalyx material that enhanced bacterial adherence) were obtained by growing the anaerobic cellulolytic bacteria Ruminococcus albus 7 or Clostridium
Autor:
Diane M. Dietrich, William R. Kenealy
Publikováno v:
Enzyme and Microbial Technology. 34:490-498
Phanerochaete chrysosporium BKM-F-1767 produced small amounts of ethanol from glucose, mannose, cellobiose, maltose and sucrose when grown with a limited O2 supply in sealed bottles. Under O2-limited growth on glucose, low levels of acetate or oxalat
Autor:
William R. Kenealy, Thomas W. Jeffries
Publikováno v:
Biotechnology Letters. 25:1619-1623
High-throughput screening requires simple assays that give reliable quantitative results. A microplate assay was developed for reducing sugar analysis that uses a 2,2′-bicinchoninic-based protein reagent. Endo-1,4-β-d-xylanase activity against oat
Publikováno v:
Mycopathologia. 152:155-158
Carpophilus freemani beetles' feeding on the fungusAspergillus nidulans was substantially inhibited when A. nidulans was transformed and induced to secrete the ribosome inactivating protein, restrictocin (genetic source: Aspergillus restrictus). No i
Autor:
John H. Fechner, Sanjay Kulkarni, William R. Kenealy, Hans W. Sollinger, Scan M. O'Herrin, William J. Burlingham, Jonathan P. Schneck
Publikováno v:
Human Immunology. 51:63-72
The light chain of HLA class I protein (beta 2m) has been expressed in Aspergillus nidulans. The cDNA of beta 2m was modified using the polymerase chain reaction to include overlapping extensions for its subsequent fusion into an Aspergillus vector.
Publikováno v:
Applied Biochemistry and Biotechnology. :267-276