Zobrazeno 1 - 10
of 10
pro vyhledávání: '"William P K, Chong"'
Publikováno v:
TrAC Trends in Analytical Chemistry. 66:1-18
Lipids play multiple key roles in biological systems, including acting as secondary energy reserves, being components of cellular membranes and participating in cell-signaling pathways. As a result, lipid defects have been implicated in the developme
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5c184663da493b88a111939fd833fed8
https://doi.org/10.1016/j.trac.2014.10.010
https://doi.org/10.1016/j.trac.2014.10.010
Autor:
Sze-Wai Ng, Kong-Peng Lam, William P. K. Chong, Andy Hee-Meng Tan, Nurhidayah Basri, Shengli Xu
Publikováno v:
Cell Reports, Vol 9, Iss 1, Pp 24-31 (2014)
Summary: Invariant natural killer T (iNKT) cells provide cognate help via CD1d to lipid antigen-presenting B cells for antibody production, but whether B cells reciprocally regulate iNKT cells remains largely unexplored. Here, we found peripheral, bu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88fd25f07c98f26bf34fb788137d2372
http://www.sciencedirect.com/science/article/pii/S2211124714007190
http://www.sciencedirect.com/science/article/pii/S2211124714007190
Publikováno v:
Metabolomics. 9:1109-1128
Microwave-assisted extraction (MAE) has emerged as an efficient extraction technique for various kinds of biological samples due to its low usage of extraction solvents and shorter extraction time. This review will focus on the recent developments an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e14b003dc3146e1c4a284add4020ba15
https://doi.org/10.1007/s11306-013-0528-7
https://doi.org/10.1007/s11306-013-0528-7
Autor:
Satty G. Reddy, Ying Swan Ho, William P. K. Chong, Chew-Kiat Heng, Miranda G.S. Yap, Niki S.C. Wong, Dong-Yup Lee, Faraaz N.K. Yusufi
Publikováno v:
Journal of Biotechnology. 147:116-121
We have established a liquid chromatography-mass spectrometry based metabolomics platform to identify extracellular metabolites in the medium of recombinant Chinese hamster ovary (CHO) fed-batch reactor cultures. Amongst the extracellular metabolites
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f24184eafcbb2e0e45b68cb74003ca06
https://doi.org/10.1016/j.jbiotec.2010.03.018
https://doi.org/10.1016/j.jbiotec.2010.03.018
Autor:
Satty G. Reddy, Lin Tang Goh, Chew-Kiat Heng, Ying Swan Ho, William P. K. Chong, Miranda G.S. Yap, Niki S.C. Wong, Faraaz N.K. Yusufi, Dong-Yup Lee
Publikováno v:
Rapid Communications in Mass Spectrometry. 23:3763-3771
A metabolomics-based approach was used to time profile extracellular metabolites in duplicate fed-batch bioreactor cultures of recombinant Chinese Hamster Ovary (CHO) cells producing monoclonal IgG antibody. Culture medium was collected and analysed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b16b5ab8504b277c4719f33845b4d028
https://doi.org/10.1002/rcm.4328
https://doi.org/10.1002/rcm.4328
Autor:
William P. K. Chong, Ying Swan Ho, Dong-Yup Lee, Meiyappan Lakshmanan, Bevan Kai-Sheng Chung, Kok Siong Ang, Ju Xin Chin, Hock Chuan Yeo
Publikováno v:
Metabolomics. 12
In pre-processing LC–MS data for metabolomics (lipidomics) profiling studies, working parameters used in many software were still prevalently based on expert recommendations which may not be generalizable or optimal for the analytical conditions on
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6f3455458f8753d18baf74cbc6306759
https://doi.org/10.1007/s11306-015-0884-6
https://doi.org/10.1007/s11306-015-0884-6
Autor:
Eric Chun Yong Chan, Shu Hui Thng, Dong-Yup Lee, Ai Ping Hiu, Ying Swan Ho, William P. K. Chong
Publikováno v:
Biotechnology and bioengineering. 109(12)
The selection of suitable mammalian cell lines with high specific productivities is a crucial aspect of large-scale recombinant protein production. This study utilizes a metabolomics approach to elucidate the key characteristics of Chinese hamster ov
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f5c433cd7ab03d5aa52c0c957481127b
https://pubmed.ncbi.nlm.nih.gov/22711553
https://pubmed.ncbi.nlm.nih.gov/22711553
Autor:
Chew-Kiat Heng, Faraaz N.K. Yusufi, Miranda G.S. Yap, Satty G. Reddy, Dong-Yup Lee, Ying Swan Ho, William P. K. Chong, Niki S.C. Wong
Publikováno v:
Journal of biotechnology. 151(2)
A liquid chromatography–mass spectrometry (LC–MS) based metabolomics platform was previously established to identify and profile extracellular metabolites in culture media of mammalian cells. This presented an opportunity to isolate novel apoptos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4908d5a1269f35d9b7c8d695792ec00
https://pubmed.ncbi.nlm.nih.gov/21167884
https://pubmed.ncbi.nlm.nih.gov/21167884
Autor:
William P K, Chong, Lin Tang, Goh, Satty G, Reddy, Faraaz N K, Yusufi, Dong Yup, Lee, Niki S C, Wong, Chew Kiat, Heng, Miranda G S, Yap, Ying Swan, Ho
Publikováno v:
Rapid communications in mass spectrometry : RCM. 23(23)
A metabolomics-based approach was used to time profile extracellular metabolites in duplicate fed-batch bioreactor cultures of recombinant Chinese Hamster Ovary (CHO) cells producing monoclonal IgG antibody. Culture medium was collected and analysed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::113285baada762f35cbe8bc491a9c154
https://pubmed.ncbi.nlm.nih.gov/19902412
https://pubmed.ncbi.nlm.nih.gov/19902412
Publikováno v:
Biotechnology progress. 25(3)
Adenosine causes growth arrest in recombinant mammalian cell cultures, which results in enhanced productivity of the recombinant protein. Adenosine is also known to increase intracellular ATP level when added to mammalian cells. As a cell's energy le
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0191a92b76e06924316fa65f699f9523
https://pubmed.ncbi.nlm.nih.gov/19507259
https://pubmed.ncbi.nlm.nih.gov/19507259