Zobrazeno 1 - 10
of 13
pro vyhledávání: '"William J. Hillegas"'
Autor:
Yae In Cho, Claire L. Armstrong, Ariana Sulpizio, Kofi K. Acheampong, Kameron N. Banks, Oishi Bardhan, Sydney J. Churchill, Annie E. Connolly-Sporing, Callie E. W. Crawford, Peter L. Cruz Parrilla, Sarah M. Curtis, Lauren M. De La Ossa, Samuel C. Epstein, Clara J. Farrehi, Grayson S. Hamrick, William J. Hillegas, Austin Kang, Olivia C. Laxton, Joie Ling, Sara M. Matsumura, Victoria M. Merino, Shahla H. Mukhtar, Neel J. Shah, Casey H. Londergan, Clyde A. Daly, Bashkim Kokona, Louise K. Charkoudian
Publikováno v:
Biochemistry
The strategic redesign of microbial biosynthetic pathways is a compelling route to access molecules of diverse structure and function in a potentially environmentally sustainable fashion. The promise of this approach hinges on an improved understandi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da3d24f7f8f9523274f3a391d4e081ac
https://doi.org/10.1021/acs.biochem.1c00798
https://doi.org/10.1021/acs.biochem.1c00798
Autor:
James Varani, Lloyd M. Stoolman, Yunhong Ding, Randall N. Knibbs, Michael K. Dame, William J. Hillegas, Melissa R. Allen
Publikováno v:
Biotechnology Progress. 19:9-13
The present study shows that COS-7 cells transiently transfected and maintained on positively charged (trimethylamine-coated) microcarrier beads synthesize recombinant protein at higher levels and for longer periods of time than cells transfected and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eabe53ec3146b2eb76dad6b8fe9ee499
https://doi.org/10.1021/bp020092r
https://doi.org/10.1021/bp020092r
Publikováno v:
Cytotechnology. 28:101-109
The present study describes a novel microcarrier substrate consisting of a swellable, copolymer of styrene and divinylbenzene, derivatized with trimethylamine. The co-polymer trimethylamine microcarriers support the growth of a number of different ce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6bc1a10ef191ae79e02986ba27799b4c
https://doi.org/10.1023/a:1008029715765
https://doi.org/10.1023/a:1008029715765
Publikováno v:
Cytotechnology. 22:111-117
Polystyrene microcarriers were prepared in four size ranges (53-63 μm, 90-125 μm, 150-180 μm and 300-355 μm) and examined for ability to support attachment and growth of human diploid fibroblasts. Cells attached rapidly to the microcarriers and t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93744fa4c1171e74b5a01e6695bae382
https://doi.org/10.1007/bf00353930
https://doi.org/10.1007/bf00353930
Publikováno v:
Cytotechnology. 13:89-98
Polystyrene culture dishes and polystyrene microcarriers were coated with Pronectin-F and poly-L-lysine (polylysine), either alone or in combination. Pronectin-F is a recombinant peptide containing repeats of the RGD cell-attachment sequence from fib
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d506accbd1cb07e2d22c0e75c0edb233
https://doi.org/10.1007/bf00749935
https://doi.org/10.1007/bf00749935
Publikováno v:
Journal of biomedical materials research. 29(8)
Human squamous epithelial cells produce lower amounts of laminin and fibronectin when cultured on DEAE-dextran than when cultured on gelatin-coated polystyrene (Biotechnol. Bioeng., 33:1235). The epithelial cells also spread much more slowly on DEAE-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e348eb5644711c27deec188b49fdafc3
https://pubmed.ncbi.nlm.nih.gov/7593043
https://pubmed.ncbi.nlm.nih.gov/7593043
Autor:
William J. Hillegas, James Varani
Publikováno v:
Animal Cell Technology: Basic & Applied Aspects ISBN: 9789401043663
Polystyrene culture dishes and polystyrene microcarriers were coated with two different peptides — Pronectin-F and polylysine, alone and in combination. Each of the two peptides alone supported cell attachment but cell spreading occurred only on Pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3c9d450cabc2ecc8c6378b139865c1cf
https://doi.org/10.1007/978-94-011-0848-5_71
https://doi.org/10.1007/978-94-011-0848-5_71
Publikováno v:
Cytotechnology. 9(1-3)
mRNA levels for urokinase type plasminogen activator (uPA), tissue type plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1) and plasminogen activator inhibitor-2 (PAI-2) were examined in human diploid (neonatal foreskin) fibroblast
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8e7c9fe6a9c2414ce5f2f663ce2174c
https://pubmed.ncbi.nlm.nih.gov/1369169
https://pubmed.ncbi.nlm.nih.gov/1369169
Publikováno v:
Journal of Biological Standardization. 13:67-76
Normal diploid human fibroblasts and first passage monkey kidney epithelial cells were examined for growth and metabolic activity on microcarriers made from glass and on microcarriers made from DEAE-dextran. The cells grew to a higher density (cells
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29ed7260706061e91bfa94afa4fcb1d7
https://doi.org/10.1016/s0092-1157(85)80035-4
https://doi.org/10.1016/s0092-1157(85)80035-4
Autor:
James Varani, Matthew J. Bendelow, David L. Helmreich, Suzanne E.G. Fligiel, Dennis R. Inman, William J. Hillegas
Publikováno v:
Biotechnology and Bioengineering. 33:1235-1241
Two human squamous carcinoma cell lines and human diploid fibroblasts were examined for the production of extracellular matrix (ECM) molecules including fibronectin (FN), laminin (LN), and thrombospondin (TSP) when grown on a number of different subs
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a9165b4dbf19ba8980995c90d0e55ae
https://doi.org/10.1002/bit.260331003
https://doi.org/10.1002/bit.260331003