Zobrazeno 1 - 10
of 12
pro vyhledávání: '"William J. Daily"'
Autor:
Michael Scurria, Terry L. Riss, Martha O'brien, William J. Daily, Laurent Bernad, Richard A. Moravec
Publikováno v:
Analytical Biochemistry. 387:294-302
A luminescent method to individually measure the chymotrypsin-like, trypsin-like, or caspase-like activities of the proteasome in cultured cells was developed. Each assay uses a specific luminogenic peptide substrate in a buffer optimized for cell pe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::061d02f7603ce0d6ca238bf5921c2a2b
https://doi.org/10.1016/j.ab.2009.01.016
https://doi.org/10.1016/j.ab.2009.01.016
Autor:
Michael Scurria, Andrew L. Niles, Terry L. Riss, P. Eric Hesselberth, Richard A. Moravec, William J. Daily
Publikováno v:
Analytical Biochemistry. 366:197-206
A method to simultaneously determine the relative numbers of live and dead cells in culture by introducing a combination of two fluorogenic substrates or a fluorogenic and a luminogenic protease substrate into the sample is described. The method is b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb4970c71cc0861f442f527979113952
https://doi.org/10.1016/j.ab.2007.04.007
https://doi.org/10.1016/j.ab.2007.04.007
Autor:
William J. Daily, Susan Frackman, Troy Good, Dongping Ma, Daniel J. Simpson, David Liu, Mary Sobol, James J. Cali
Publikováno v:
Expert Opinion on Drug Metabolism & Toxicology. 2:629-645
Luminogenic cytochrome P450 (CYP) assays couple CYP enzyme activity to firefly luciferase luminescence in a technology called P450-Glo(TM) (Promega). Luminogenic substrates are used in assays of human CYP1A1, -1A2, -1B1, -2C8, -2C9, -2C19, -2D6, -2J2
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::54617edb66767da989da8cdda5b8dbf0
https://doi.org/10.1517/17425255.2.4.629
https://doi.org/10.1517/17425255.2.4.629
Autor:
Michael Scurria, Martha O'brien, Keith V. Wood, William J. Daily, Robert F. Bulleit, P. Eric Hesselberth, Richard A. Moravec, Dieter Klaubert
Publikováno v:
SLAS Discovery. 10:137-148
1 Using caspase-3 as a model, the authors have developed a strategy for highly sensitive, homogeneous protease assays suitable for high-throughput, automated applications. The assay uses peptide-conjugated aminoluciferin as the protease substrate and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cfcd9d3d8878f7ddec2d8cd3a435a148
https://doi.org/10.1177/1087057104271865
https://doi.org/10.1177/1087057104271865
Autor:
Timothy A. Riley, Lyle J. Arnold, Michael Scurria, William B. Marvin, Christine D. Garcia, Stephanie A. Hopkins, William J. Daily, David A. Schwartz, Michael C. Pirrung, Michael B. Atkins
Publikováno v:
Nucleosides and Nucleotides. 16:417-432
A diastereoselective dinucleoside methylphosphonate synthetic method that features coupling of diastereomerically pure 1,1,1,3,3,3-hexafluoro-2-propyl nucleoside-3′-O-methylphosphonate monomers with 3′-O-protected nucleoside monomers mediated by
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::805906a537151e5e215353bcb1f650bc
https://doi.org/10.1080/07328319708001359
https://doi.org/10.1080/07328319708001359
Autor:
Timothy A. Riley, Mark A. Reynolds, Terry A. Beck, Lyle J. Arnold, Susan K. Knowles, Morteza M. Vaghefi, William B. Marvin, David A. Schwartz, Richard I. Hogrefe, William J. Daily, Robert E. Klem, John A. Jaeger
Publikováno v:
Nucleic Acids Research. 24:4584-4591
Methylphosphonate (MP) oligodeoxynucleotides (MPOs) are metabolically stable analogs of conventional DNA containing a methyl group in place of one of the non-bonding phosphoryl oxygens. All 16 possible chiral R(P) MP dinucleotides were synthesized an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25b7be4874f51fb8b7ecc3e0d30b3b59
https://doi.org/10.1093/nar/24.22.4584
https://doi.org/10.1093/nar/24.22.4584
Autor:
Philip B. Say, Terry A. Beck, Morteza M. Vaghefi, Mark Alan Reynolds, Brian Patrick Dwyer, David A. Schwartz, Michael D. Metzler, Lyle J. Arnold, Robert E. Klem, William J. Daily
Publikováno v:
Nucleic Acids Research. 24:760-765
We have designed and synthesized a series of novel antisense methylphosphonate oligonucleotide (MPO) cleaving agents that promote site-specific cleavage on a complementary RNA target. These MPOs contain a non- nucleotide-based linking moiety near the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09174fe2b750273d00554f7606c31660
https://doi.org/10.1093/nar/24.4.760
https://doi.org/10.1093/nar/24.4.760
Autor:
Timothy A. Riley, C. D. Garcia, M. C. Pirrung, M. B. Atkins, Michael Scurria, William B. Marvin, S. A. Hopkins, William J. Daily, David A. Schwartz, L. J. Jun. Arnold
Publikováno v:
ChemInform. 28
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a5cf126970818b72410cf90b8e95e8ca
https://doi.org/10.1002/chin.199740274
https://doi.org/10.1002/chin.199740274
Autor:
James J. Cali, Carolyn C. Woodroofe, Dieter Klaubert, Jean Osterman, William J. Daily, Poncho Meisenheimer, Monika G. Wood, John Shultz
Publikováno v:
Biochemistry. 47(39)
A set of 6'-alkylated aminoluciferins are shown to be bioluminescent substrates for Ultra-Glo and QuantiLum luciferases. These studies demonstrate that both the engineered and wild-type firefly luciferases tolerate much greater steric bulk at the 6'
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6157e4a33a08710da306503345ff3d8b
https://pubmed.ncbi.nlm.nih.gov/18771284
https://pubmed.ncbi.nlm.nih.gov/18771284
Autor:
Martha A. OBrien, William J. Daily, P. Eric Hesselberth, Richard A. Moravec, Michael A. Scurria, Dieter H. Klaubert, Robert F. Bulleit, Keith V. Wood
Publikováno v:
Journal of Biomolecular Screening; Mar2005, Vol. 10 Issue 2, p137-148, 12p