A phylogenetic approach for weighting genetic sequences

Autor: Marc A. Suchard, William J. Coleman-Smith, Fabio Pardi, Alexander V. Alekseyenko, Nicola De Maio, Asif U. Tamuri, Jakub Truszkowski, Nick Goldman

Publikováno v: BMC bioinformatics, vol 22, iss 1
BMC Bioinformatics
BMC Bioinformatics, Vol 22, Iss 1, Pp 1-24 (2021)
BMC Bioinformatics, BioMed Central, 2021, 22 (1), pp.#285. ⟨10.1186/s12859-021-04183-8⟩

Background Many important applications in bioinformatics, including sequence alignment and protein family profiling, employ sequence weighting schemes to mitigate the effects of non-independence of homologous sequences and under- or over-representati

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::084ec05dba21d883533e341b44e0c54d
https://escholarship.org/uc/item/7rg237jg

Site-directed Mutations at D1-Thr179 of Photosystem II in Synechocystis sp. PCC 6803 Modify the Spectroscopic Properties of the Accessory Chlorophyll in the D1-branch of the Reaction Center

Autor: Eberhard Schlodder, Thomas Renger, Bruce A. Diner, Peter J. Nixon, Grzegorz Raszewski, Rachel O. Cohen, William J. Coleman

Publikováno v: Biochemistry. 47:3143-3154

D1-Thr179, which overlies the reaction center chlorophyll Chl D1 of Photosystem II was replaced with His and Glu through site-directed mutation in Synechocystis sp. PCC 6803. Spectroscopic characterization of the mutants indicates that, compared to w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d63043429e58b26635ad7b56fed92cc
https://doi.org/10.1021/bi702059f

Application of a very high-throughput digital imaging screen to evolve the enzyme galactose oxidase

Autor: Christina L. Grek, Simon Delagrave, Michael R. Dilworth, William J. Coleman, Dennis J. Murphy, Anthony M. Maffia, Douglas C. Youvan, Mary M. Yang, Edward J. Bylina, Jennifer L. Rittenhouse Pruss, Barry L. Marrs

Publikováno v: Protein Engineering, Design and Selection. 14:261-267

Directed evolution has become an important enabling technology for the development of new enzymes in the chemical and pharmaceutical industries. Some of the most interesting substrates for these enzymes, such as polymers, have poor solubility or form

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b86e523258c10ba8ee5e40b257a5ca9
https://doi.org/10.1093/protein/14.4.261

Engineering highly thermostable xylanase variants using an enhanced combinatorial library method

Autor: Roberto Crea, Craig A. Behnke, William J. Coleman, Guido Cappuccilli, Craig A. Hokanson, Michael Mendez, Tatjana Odineca, Marino Bozic

Publikováno v: Protein engineering, designselection : PEDS. 24(8)

A new directed evolution method was used to enhance the thermostability of the wild-type GH11 xylanase 2 (known as BD-11) from Hypocrea jecorina (Trichoderma reesei). Both Look-Through Mutagenesis (LTM™), which is a method for rapidly screening sel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55a2cb9ec88e2d68946a843d9bd1f1e1
https://pubmed.ncbi.nlm.nih.gov/21708791

Spectroscopic Analysis of Genetically Modified Photosynthetic Reaction Centers

Autor: William J. Coleman, Douglas C. Youvan

Publikováno v: Annual Review of Biophysics and Biophysical Chemistry. 19:333-367

REPLACEMENT OF THE AXIAL LIGANDS TO THE MONOMERIC BACTERIOCHLOROPHYLLS . . . . . . 349 THE SPECTROSCOPIC RED SHIFT OF THE PHOTOACTIVE BACTERIOPHEOPHYTIN . . . . . . . 351 THE QUINONE ELECTRON ACCEPTORS ....... . . . . ..... ....... .... 354 Electron

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e10edef2cb6a5ed77aa078bd0057cbf
https://doi.org/10.1146/annurev.bb.19.060190.002001