Zobrazeno 1 - 10
of 56
pro vyhledávání: '"William H. J. Douglas"'
Publikováno v:
Tissue and Cell. 15:193-204
Bovine pulmonary artery endothelial cells in culture were evaluated by phase-contrast and electron microscopy at various times after being irradiated with 137 Cs in vitro . Cells irradiated prior to reaching confluency showed vacuolization and increa
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 664:380-388
Organotypic cultures of pulmonary type II epithelial cells were treated with dexamethasone at concentrations between 10 −10 and 10 −5 M for 48 h followed by a 3 h incubation in 5.6 mM [U- 14 C]glucose. A surfactant and a residual fraction was iso
Publikováno v:
In Vitro. 12:74-82
Human diploid fibroblasts can be maintained in vitro in an arrested, essentially nonmitotic state for extended periods of time by reducing the serum concentration in the medium from 10 to 0.5%. Arrested cells can be induced to re-enter the proliferat
Autor:
Louis Terracio, William H. J. Douglas
Publikováno v:
The Prostate. 3:183-191
This study describes a method for establishing primary cultures in 24-well culture vessels and evaluating the effects of serum, hormones, and other factors on cell growth using densitometry. Primary cultures of rat ventral prostate epithelial cells w
Publikováno v:
In Vitro. 12:373-381
Enzymatically dissociated lungs from rat fetuses at 19-days gestation yield single cells which reaggregate to form alveolar-like structures when cultured on gelatin sponge discs. These structures form within 2 days and have been maintained in vitro f
Publikováno v:
Journal of Tissue Culture Methods. 7:85-88
A simple and convenient method is described to aid the processing of minute tissue specimens and isolated single cells for scanning electron microscopy (SEM). The technique employs an inexpensive, disposable carrier fashioned from common Beem capsule
Publikováno v:
Tissue and Cell. 7:137-142
When rat pulmonary tissues are processed as outlined in this paper, the type II cell’s surfactant containing osmiophilic inclusion bodies possess a periodic substructure. This substructure consists of electron-opaque lamellae, 26 A in thickness alt
Publikováno v:
In Vitro. 13:55-62
Mitotic activity in confluent cultures of human diploid fibroblasts was arrested by the reduction of the serum concentration of the incubation medium to 0.5% or by the addition of 0.5 mM 6-N, 2'-O-dibutyryl-adenosine 3':5'-cyclic monophosphate (db cA
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 572:502-509
Prostaglandin production was studied in fetal and adult type II alveolar epithelial cells. Two culture systems were employed, fetal rat lung organotypic cultures consisting of fetal type II cells and monolayer cultures of adult lung type II cells. De
Publikováno v:
Journal of Andrology. 3:269-280
Stromal-epithelial cell interactions may be important for the regulation of normal and abnormal prostatic growth. While androgen transformation by rat ventral prostate and canine prostate has been investigated in vivo and in organ culture, little is