Current State of Oligonucleotide Characterization Using Liquid Chromatography–Mass Spectrometry: Insight into Critical Issues

Autor: William D van Dongen, Vidya Annavarapu, J. Michael Sutton, Guilherme J Guimaraes, Michael G. Bartlett

Publikováno v: Journal of the American Society for Mass Spectrometry. 31:1775-1782

As interests increase in oligonucleotide therapeutics, there has been a greater need for analytical techniques to properly analyze and quantitate these biomolecules. This article looks into some of the existing chromatographic approaches for oligonuc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a3cb9ec2859a8927edd1e6c09905ffe
https://doi.org/10.1021/jasms.0c00179

Quantitative bottom up analysis of infliximab in serum using protein A purification and integrated μLC-electrospray chip IonKey MS/MS technology

Autor: Frédérique L. van Holthoon, Jan H. Toersche, Martha Ingola, William D van Dongen, Anne J. Kleinnijenhuis

Publikováno v: Bioanalysis. 8:891-904

Background: TNO Triskelion has applied its general workflow for the development of quantitative LC–MS methods for proteins in biological matrices to the quantification of infliximab in rat serum using bottom up μLC–MS/MS. Results/methodology: Th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0457da3ac4093ff4d53d4645c5172fd8
https://doi.org/10.4155/bio-2015-0015

Practical Considerations for the Pharmacokinetic and Immunogenic Assessment of Antibody–Drug Conjugates

Autor: William D van Dongen, Melody Sauerborn

Publikováno v: BioDrugs. 28:383-391

Currently, the most bioanalytically challenging drugs are antibody-drug conjugates (ADCs), constructs comprising a monoclonal antibody and a cytotoxic drug connected by a linker. The bioanalytical challenges arise from the heterogeneous nature of ADC

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cd1aacb41f19eb3f46231b46df1da6d
https://doi.org/10.1007/s40259-014-0096-z

Bioanalytical LC–MS of therapeutic oligonucleotides

Autor: Wilfried M. A. Niessen, William D van Dongen

Publikováno v: van Dongen, W D & Niessen, W M A 2011, ' Bioanalytical LC-MS of therapeutic oligonucleotides ', Bioanalysis, vol. 3, pp. 541-564 . https://doi.org/10.4155/bio.11.8
Bioanalysis, 3, 541-564. Future Science

Therapeutic oligonucleotides (OGNTs) are important biopharmaceutical drugs for the future, due to their ability to selectively reduce or knockout the expression of target genes. For the development of OGNTs, reliable and relatively high-throughput bi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70409b8cdb46ab0610334a8436818d2f
https://doi.org/10.4155/bio.11.8

Exploring the boundaries of bioanalytical quantitative LC–MS–MS

Autor: Jan H.G Jonkman, Ruud J.J.M Steenvoorden, William D van Dongen, Fija M. Lagerwerf, Maarten Honing

Publikováno v: TrAC Trends in Analytical Chemistry. 19:418-427

In this article, the boundaries of quantitative high-performance liquid chromatography coupled with tandem mass spectrometry (LC–MS–MS) as a bioanalytical tool for clinical pharmacology studies are described. Although LC–MS–MS is a universal,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::aee17c7ebaeec49e4b88d98aa1ff2dc4
https://doi.org/10.1016/s0165-9936(00)00009-1