Zobrazeno 1 - 7
of 7
pro vyhledávání: '"William C.A. Tacon"'
Publikováno v:
FEMS Microbiology Letters. 56:101-104
The plasmid pAT153 was lost less rapidly from carbon, nitrogen, phosphorous or sulphur-limited continuous cultures of Escherichia coli HB101 as the dilution rate increased. At a fixed dilution rate of 0.3 h−1, the plasmid was maintained longer as t
Publikováno v:
Gene. 23:255-265
Two DNA fragments which contain the Escherichia coli tryptophan promoter operator region but lack the attenuator have been used in the construction of a series of pAT153 based plasmids suitable for the regulated expression of foreign genes in E. coli
Publikováno v:
Gene. 46:103-112
It has been found that with high-copy-number vectors utilising the Escherichia coli trp promoter the amount of represser protein produced from the single chromosomally located trpR gene is inadequate for tight repression to be obtained. An attempt ha
Autor:
K. Dawson, C. Hynes, L. Dunthorne, Alan George Porter, William C.A. Tacon, J. Viney, N. Warburton, M. Finlay, S.M. Doel, M. Houghton, A.G. Stewart, M. Nugent, J.A. Davies, J.R. Adair, G.J. O'neill, L.D. Bell, J. Clarke, Keith Graham Mccullagh, R. Derbyshire, P.G. Boseley, J. Hall, I. Lindley, G. H. Catlin, J. C. Smith
Publikováno v:
DNA. 5:137-148
A series of novel, modified interferons based on the structure of human beta-interferon have been expressed in Escherichia coli. Modified interferon genes were constructed from sequences derived from the natural beta-interferon gene, a synthetic beta
Publikováno v:
Microbiology. 132:1021-1026
Summary: We have characterized a copy number mutant of the pBR322-based plasmid pWT111. A single nucleotide transversion in loop II’ of RNAI results in an eightfold increase in plasmid copy number. Removal of the rom coding region from pWT111cop re
Autor:
B. Jenkins, John Spencer Emtage, Norman Henry Carey, William C.A. Tacon, Alan George Porter, G. H. Catlin
Publikováno v:
Nature. 283:171-174
A gene sequence for the fowl plague virus (FPV) haemagglutinin molecule has been inserted into a bacterial plasmid such that its transcription is under the control of a promoter derived from the tryptophan operon. Such plasmids direct the synthesis o
Autor:
Christopher M. Thomas, Simon R. Warne, Bimal D.M. Theophilus, William C.A. Tacon, Michael Pinkney
Publikováno v:
Plasmid. 17(3)
Reverse transcriptase mapping has been used to analyze transcription from the trfA promoter of broad host range plasmid RK2. The results show that trfA operon mRNA has the same 5' end in Pseudomonas aeruginosa, Pseudomonas putida, and Escherichia col