Zobrazeno 1 - 10
of 18
pro vyhledávání: '"William Bradshaw Amos"'
Publikováno v:
eLife, Vol 5 (2016)
Current optical microscope objectives of low magnification have low numerical aperture and therefore have too little depth resolution and discrimination to perform well in confocal and nonlinear microscopy. This is a serious limitation in important a
Externí odkaz:
https://doaj.org/article/e771731c9502434f8bcf423c302fe30c
Autor:
Rumelo Amor, Alison McDonald, Johanna Trägårdh, Gillian Robb, Louise Wilson, Nor Zaihana Abdul Rahman, John Dempster, William Bradshaw Amos, Trevor J Bushell, Gail McConnell
Publikováno v:
PLoS ONE, Vol 11, Iss 1, p e0147115 (2016)
We demonstrate fluorescence imaging by two-photon excitation without scanning in biological specimens as previously described by Hwang and co-workers, but with an increased field size and with framing rates of up to 100 Hz. During recordings of synap
Externí odkaz:
https://doaj.org/article/6d6924beec5e43e6bb67dd3e7e8b7334
Autor:
Shannan Foylan, William Bradshaw Amos, John Dempster, Lisa Koelln, Carsten G. Hansen, Michael J. Shaw, Gail McConnell
Publikováno v:
Single Molecule Spectroscopy and Superresolution Imaging XVI.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7cee8da16976f7a7f156a03fd62d9e97
https://doi.org/10.1117/12.2649070
https://doi.org/10.1117/12.2649070
Publikováno v:
The ISME Journal
The ability of microorganisms to grow as aggregated assemblages has been known for many years, however their structure has remained largely unexplored across multiple spatial scales. The development of the Mesolens, an optical system which uniquely a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c80be5a4e55f8addc2e4f1c82477997
https://doi.org/10.1038/s41396-020-0700-9
https://doi.org/10.1038/s41396-020-0700-9
Autor:
William Bradshaw Amos, Gail McConnell, Lisa S. Kölln, Liam M. Rooney, Ross Scrimgeour, Paul A. Hoskisson
Publikováno v:
mSphere
mSphere, Vol 5, Iss 1 (2020)
mSphere, Vol 5, Iss 1, p e00846-19 (2020)
mSphere, Vol 5, Iss 1 (2020)
mSphere, Vol 5, Iss 1, p e00846-19 (2020)
3D imaging of live bacteria with optical microscopy techniques is a challenge due to the small size of bacterial cells, meaning that previous studies have been limited to observing motility behavior in 2D. We introduce the application of confocal mul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6c2793433701df76e81858475eb0837
https://pubmed.ncbi.nlm.nih.gov/31996414
https://pubmed.ncbi.nlm.nih.gov/31996414
Biofilms are important in medicine, industry and the natural environment, however their structure is largely unexplored across multiple spatial scales. We have studied the architecture of matureEscherichia colimacro-colony biofilms by means of the Me
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f03513fdeec8c4c0d7767a3d3bad9d18
Autor:
Ross Scrimgeour, Liam M. Rooney, William Bradshaw Amos, Gail McConnell, Paul A. Hoskisson, Lisa S. Kölln
The Delta-proteobacterium,Myxococcus xanthus, has been used as a model for bacterial motility and to provide insights of bacterial swarming behaviours. Fluorescence microscopy techniques have shown that various mechanisms are involved in gliding moti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::814ebb3350f81b18b949d4a0178676a1
Autor:
Trevor J. Bushell, John Dempster, Aimee Franssen, William Bradshaw Amos, Jan Schniete, Gail McConnell
Publikováno v:
Scientific Reports
Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
We present here a fast optical sectioning method for optical mesoscopy based on HiLo microscopy, which makes possible imaging of specimens of up to 4.4 mm × 3 mm × 3 mm in volume in under 17 hours (estimated for a z-stack comprising 1000 images exc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9a21d0e6dae293c0630de01e0a96b79
https://pubmed.ncbi.nlm.nih.gov/30390029
https://pubmed.ncbi.nlm.nih.gov/30390029
Autor:
William Bradshaw Amos, Gail McConnell
In a previous paper (McConnell et al., 2016) we showed a new giant lens called the Mesolens and presented performance data and images from whole fixed and intact fluorescently-stained 12.5-day old mouse embryos. Here we show that using the Mesolens w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f305460c7d3206d4e9654b9507f125ed
Autor:
Rumelo Amor, John Dempster, Gail McConnell, Gillian Robb, William Bradshaw Amos, Johanna Trägårdh
Publikováno v:
Journal of Microscopy. 259:210-218
We have studied the wavelength dependence of the two-photon excitation efficiency for a number of common UV excitable fluorescent dyes; the nuclear stains DAPI, Hoechst and SYTOX Green, chitin- and cellulose-staining dye Calcofluor White and Alexa Fl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::309e51d3eaddb93baada588aea47119a
https://doi.org/10.1111/jmi.12255
https://doi.org/10.1111/jmi.12255