Zobrazeno 1 - 10
of 32
pro vyhledávání: '"Willfried Schramm"'
Autor:
Willfried Schramm, Gundula Voss
Publikováno v:
Helvetica Chimica Acta. 83:2884-2892
Selectively C-deuterated indigotins were synthesized from halogenated indigotins 1a – c as precursors. The described reactions – used already by the ancient purple dyers – allowed the introduction of D-atoms at defined positions of the indigo m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e5f6b9131b502cb61412b4ced594fbd2
https://doi.org/10.1002/1522-2675(20001108)83:11<2884::aid-hlca2884>3.0.co
https://doi.org/10.1002/1522-2675(20001108)83:11<2884::aid-hlca2884>3.0.co
Autor:
Willfried Schramm, Se-Hwan Paek
Publikováno v:
Biotechnology and Bioengineering. 56:221-231
Factors that control the performance of a reversible immunosensor with an analyte (progesterone)-enzyme (horseradish peroxidase) conjugate as signal generator have been investigated. The conjugate is used in conjunction with two antibodies, which are
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4caeb3c7b4c2760f54b092940bbbc58e
https://doi.org/10.1002/(sici)1097-0290(19971020)56:2<221::aid-bit11>3.0.co
https://doi.org/10.1002/(sici)1097-0290(19971020)56:2<221::aid-bit11>3.0.co
Publikováno v:
Behavior Research Methods, Instruments, & Computers. 27:470-475
Cortisol can be used to indicate stress level as well as to monitor certain disease states. Although cortisol can be sampled from blood and urine, saliva sampling has decided advantages. Unfortunately, whole saliva contains a number of substances tha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::913c8707ae81ee5a50c52aac0377a1e3
https://doi.org/10.3758/bf03200445
https://doi.org/10.3758/bf03200445
Publikováno v:
ImmunoMethods. 3:93-103
The immobilization of antibodies in two-dimensional (physical adsorption and immobilization via binding proteins) and that in three-dimensional (chemical binding to polypeptide strands) coverage of solid matrices have been compared. Methods of optimi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fd26c294ae11053f7f87f8b9b0dba1d6
https://doi.org/10.1006/immu.1993.1043
https://doi.org/10.1006/immu.1993.1043
Publikováno v:
Analytical Biochemistry. 210:145-154
We investigated the synthesis of progesterone-horseradish peroxidase (P-HRP) conjugates and products purified by affinity chromatography. The obtained preparations were characterized with an immobilized monoclonal antibody in solid-phase immunoassays
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d08904134aaf32735940a9ac7dc88335
https://doi.org/10.1006/abio.1993.1165
https://doi.org/10.1006/abio.1993.1165
Autor:
Willfried Schramm, Se-Hwan Paek
Publikováno v:
Analytical Biochemistry. 205:47-56
We have investigated the complex formation between an immobilized monoclonal antibody and antigens that differ in size about 50-fold. As a model system, we used an iodinated progesterone derivative and a progesterone-horseradish peroxidase conjugate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::114e677ca26e8355c47a21ed35e043dc
https://doi.org/10.1016/0003-2697(92)90577-t
https://doi.org/10.1016/0003-2697(92)90577-t
Autor:
Se-Hwan Paek, Willfried Schramm
Publikováno v:
Analytical Biochemistry. 196:326-336
In an attempt to optimize immunosensors operating with an immobilized antibody as binding protein and an analyte-enzyme conjugate as signal generator that is significantly larger in molecular size than the analyte, in a previous communication (Part I
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a5c419f28184e520b27c369c2f576a9
https://doi.org/10.1016/0003-2697(91)90474-8
https://doi.org/10.1016/0003-2697(91)90474-8
Publikováno v:
Analytica Chimica Acta. 248:517-528
A device for the in situ collection of an ultrafiltrate of saliva was investigated. The collector consists of an osmotic pump that, when placed in the mouth, accumulates a prepurified salivary filtrate within a few minutes. The concentration of testo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ecc15e6d3a9cf7bd89056726a5d478d7
https://doi.org/10.1016/s0003-2670(00)84670-x
https://doi.org/10.1016/s0003-2670(00)84670-x
Publikováno v:
Clinical Chemistry. 36:1360-1363
We describe a simple method for estimating the association constant between an antibody and an antigen, based on a theoretical treatment of experimental dose-response data. We used this method to calculate the association constants of an anti-progest
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0fa7ce0ed5aaf7366e212b51a2e83911
https://doi.org/10.1093/clinchem/36.7.1360
https://doi.org/10.1093/clinchem/36.7.1360
Publikováno v:
Clinical Chemistry. 36:509-514
We describe, for the measurement of 6-keto prostaglandin F1 alpha in biological media, a solid-phase immunoassay with immobilized antibodies that requires a total processing time of less than 2 h with hands-on time less than 30 min for 40 samples. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::53d20119f6ffba9ae5e217a8c688fe03
https://doi.org/10.1093/clinchem/36.3.509
https://doi.org/10.1093/clinchem/36.3.509