Zobrazeno 1 - 10
of 44
pro vyhledávání: '"Willem P. C. Stemmer"'
Autor:
Susan E Alters, Bryant McLaughlin, Benjamin Spink, Tigran Lachinyan, Chia-Wei Wang, Vladimir Podust, Volker Schellenberger, Willem P C Stemmer
Publikováno v:
PLoS ONE, Vol 8, Iss 6 (2013)
[This corrects the article on p. e50630 in vol. 7.].
Externí odkaz:
https://doaj.org/article/284b942b9e2548579d164be13a0cbbea
Autor:
Susan E Alters, Bryant McLaughlin, Benjamin Spink, Tigran Lachinyan, Chia-wei Wang, Vladimir Podust, Volker Schellenberger, Willem P C Stemmer
Publikováno v:
PLoS ONE, Vol 7, Iss 11, p e50630 (2012)
OBJECTIVES: Glucagon-like peptide 2 (GLP2) is an intestinal growth factor that has been shown to stimulate intestinal growth and reduce disease severity in preclinical models of short bowel syndrome and inflammatory bowel disease. Teduglutide, a reco
Externí odkaz:
https://doaj.org/article/c35a68346617468b8b387601a1b942d1
Autor:
Nathan C Geething, Wayne To, Benjamin J Spink, Michael D Scholle, Chia-wei Wang, Yong Yin, Yi Yao, Volker Schellenberger, Jeffrey L Cleland, Willem P C Stemmer, Joshua Silverman
Publikováno v:
PLoS ONE, Vol 5, Iss 4, p e10175 (2010)
While the majority of current diabetes treatments focus on reducing blood glucose levels, hypoglycemia represents a significant risk associated with insulin treatment. Glucagon plays a major regulatory role in controlling hypoglycemia in vivo, but it
Externí odkaz:
https://doaj.org/article/5d3650dde8fd4000aca777c7b8cd018c
Autor:
Susan E. Alters, Jeffrey L. Cleland, Jerome A. Moore, Brian C. Rogers, Benjamin Spink, Chai-Wei Wang, Volker Schellenberger, Willem P. C. Stemmer, Nathan Geething
Publikováno v:
Journal of Pharmaceutical Sciences
A novel recombinant human growth hormone (rhGH) fusion protein (VRS-317) was designed to minimize receptor-mediated clearance through a reduction in receptor bind- ing without mutations to rhGH by genetically fusing with XTEN amino acid sequences to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::feeb1bd8d1084801cd05df9da0b24f5e
https://doi.org/10.1002/jps.23229
https://doi.org/10.1002/jps.23229
Autor:
Katja Pekrun, Malcolm A. Martin, Willem P. C. Stemmer, Nay-Wei Soong, Tatsuhiko Igarashi, Phillip A. Patten, Riri Shibata, Liana Sheppard, Margaret Reed
Publikováno v:
Journal of Virology. 76:2924-2935
DNA shuffling facilitated the evolution of a human immunodeficiency virus type 1 (HIV-1) variant with enhanced replication in pig-tailed macaque peripheral blood mononuclear cells (pt mPBMC). This variant consists exclusively of HIV-1-derived sequenc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c773f59082f7fd5e0eb8a8e01a1ed2a6
https://doi.org/10.1128/jvi.76.6.2924-2935.2002
https://doi.org/10.1128/jvi.76.6.2924-2935.2002
Autor:
Joost A. Kolkman, Willem P. C. Stemmer
Publikováno v:
Nature Biotechnology. 19:423-428
Evolution of eukaryotes is mediated by sexual recombination of parental genomes. Crossovers occur in random, but homologous, positions at a frequency that depends on DNA length. As exons occupy only 1% of the human genome and introns about 24%, by fa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a98fa1378d38779e0274799c781fd172
https://doi.org/10.1038/88084
https://doi.org/10.1038/88084
Publikováno v:
Nature Structural Biology. 8:238-242
Directed evolution can be a powerful tool to predict antibiotic resistance. Resistance involves the accumulation of mutations beneficial to the pathogen while maintaining residue interactions and core packing that are critical for preserving function
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::56afb6369e4772e3769d2b0993a7fc1d
https://doi.org/10.1038/84981
https://doi.org/10.1038/84981
Autor:
Nay-Wei Soong, Rebecca McKee, Anne Pinkstaff, Irina Burimski, Michael Pensiero, Willem P. C. Stemmer, Michele Kaloss, Sharon K. Powell, Edward Otto
Publikováno v:
Nature Biotechnology. 18:1279-1282
Manufacturing of retroviral vectors for gene therapy is complicated by the sensitivity of these viruses to stress forces during purification and concentration. To isolate viruses that are resistant to these manufacturing processes, we performed breed
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f0dd3d3ce6f2b9ed8564368d46e7ec93
https://doi.org/10.1038/82391
https://doi.org/10.1038/82391
Autor:
Halina Szadkowski, Astrid Merz, Andreas Crameri, Kasper Kirschner, Charles Yanofsky, Muh-ching Yee, Günter Pappenberger, Willem P. C. Stemmer
Publikováno v:
Biochemistry. 39:880-889
Enzymes from thermophilic organisms often are barely active at low temperatures. To obtain a better understanding of this sluggishness, we used DNA shuffling to mutagenize the trpC gene, which encodes indoleglycerol phosphate synthase, from the hyper
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::062188de582895695e1dbf5763409551
https://doi.org/10.1021/bi992333i
https://doi.org/10.1021/bi992333i
Autor:
Phillip A. Patten, Teddy Chen, Glenn Dawes, Brett W. Cox, Juha Punnonen, Chia-Chun J. Chang, Willem P. C. Stemmer
Publikováno v:
Nature Biotechnology. 17:793-797
DNA shuffling of a family of over 20 human interferon-alpha (Hu-IFN-alpha) genes was used to derive variants with increased antiviral and antiproliferation activities in murine cells. A clone with 135,000-fold improved specific activity over Hu-IFN-a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a781fcada2476c4715e8537728c5987
https://doi.org/10.1038/11737
https://doi.org/10.1038/11737