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pro vyhledávání: '"Willem P. Antheunisse"'
Autor:
Willem P. Antheunisse, Sven Branner, Hans Peters, Peter Ravestein, Maarten R. Egmond, Carla J. van Bemmel, Jacob de Vlieg
Publikováno v:
"Protein Engineering, Design and Selection". 7:793-800
The introduction or removal of multiple charged amino acid residues in the subtilisin Savinase by genetic engineering allowed us to modify the electrostatic properties of this enzyme in a systematic way. The effects of these charge changes were inves