Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering

Autor: Willem J. de Grip, Srividya Ganapathy

Publikováno v: Frontiers in Chemistry, Vol 10 (2022)

The first member and eponym of the rhodopsin family was identified in the 1930s as the visual pigment of the rod photoreceptor cell in the animal retina. It was found to be a membrane protein, owing its photosensitivity to the presence of a covalentl

Externí odkaz: https://doaj.org/article/56817ff8ba4b4a0797fe6fe67d58640a

Photoreaction Dynamics of Red-Shifting Retinal Analogues Reconstituted in Proteorhodopsin

Autor: Yusaku Hontani, Willem J. de Grip, Sean Frehan, Miroslav Kloz, Srividya Ganapathy, John T. M. Kennis

Publikováno v: Hontani, Y, Ganapathy, S, Frehan, S, Kloz, M, De Grip, W J & Kennis, J T M 2019, ' Photoreaction Dynamics of Red-Shifting Retinal Analogues Reconstituted in Proteorhodopsin ', Journal of Physical Chemistry B, vol. 123, no. 19, pp. 4242-4250 . https://doi.org/10.1021/acs.jpcb.9b01136
Journal of Physical Chemistry B: Condensed Matter, Materials, Surfaces, Interfaces and Biophysical, 123, 19, pp. 4242-4250
Journal of Physical Chemistry B: Condensed Matter, Materials, Surfaces, Interfaces and Biophysical, 123, 4242-4250
Journal of Physical Chemistry B
Journal of Physical Chemistry B, 123(19), 4242-4250
Journal of Physical Chemistry B, 123(19), 4242-4250. American Chemical Society

Microbial rhodopsins constitute a key protein family in optobiotechnological applications such as optogenetics and voltage imaging. Spectral tuning of rhodopsins into the deep-red and near-infrared spectral regions is of great demand in such applicat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::931b94935c5df33535ff1625bd0b8ff1
https://doi.org/10.1021/acs.jpcb.9b01136

Membrane matters: The impact of a nanodisc-bilayer or a detergent microenvironment on the properties of two eubacterial rhodopsins

Autor: Laura Opdam, Sean Frehan, Srividya Ganapathy, Que Chen, Huub J. M. de Groot, Klaas J. Hellingwerf, John T. M. Kennis, Willem J. de Grip, Yusaku Hontani

Publikováno v: Ganapathy, S, Opdam, L, Hontani, Y, Frehan, S, Chen, Q, Hellingwerf, K J, de Groot, H J M, Kennis, J T M & de Grip, W J 2020, ' Membrane matters : The impact of a nanodisc-bilayer or a detergent microenvironment on the properties of two eubacterial rhodopsins ', Biochimica et Biophysica Acta-Biomembranes, vol. 1862, no. 2, 183113, pp. 1-13 . https://doi.org/10.1016/j.bbamem.2019.183113
Biochimica et Biophysica Acta. Biomembranes, 1862
BBA-Biomembranes, 1862(2), 183113
BBA-Biomembranes
Biochimica et Biophysica Acta-Biomembranes, 1862(2):183113, 1-13. Elsevier
Biochimica et Biophysica Acta. Biomembranes, 1862, 2
Biochimica et Biophysica Acta-Biomembranes, 1862(2)

Multi-spanning membrane proteins usually require solubilization to allow proper purification and characterization, which generally impairs their structural and functional integrity. We have tested the efficacy of several commonly used detergents and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a18ac146f6aaecdcad07ccdafbf95a1
https://pubmed.ncbi.nlm.nih.gov/31672539

Redshifted and Near-infrared Active Analog Pigments Based upon Archaerhodopsin-3

Autor: Klaas J. Hellingwerf, SJ Kratz, Huub J. M. de Groot, Kenneth J. Rothschild, Willem J. de Grip, Srividya Ganapathy, Que Chen

Publikováno v: Photochemistry and Photobiology, 95, 959-968
Photochemistry and Photobiology, 95(4), 959-968
Photochemistry and Photobiology, 95, 4, pp. 959-968
Photochemistry and Photobiology

Archaerhodopsin‐3 (AR3) is a member of the microbial rhodopsin family of hepta‐helical transmembrane proteins, containing a covalently bound molecule of all‐trans retinal as a chromophore. It displays an absorbance band in the visible region of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f085102f7e92c67a6908a5c8f2c6ee23
http://hdl.handle.net/2066/206251

Combining retinal-based and chlorophyll-based (oxygenic) photosynthesis: Proteorhodopsin expression increases growth rate and fitness of a ∆PSI strain of Synechocystis sp. PCC6803

Autor: Otilia Cheregi, Srividya Ganapathy, Christiane Funk, Jos C. Arents, Willem J. de Grip, Klaas J. Hellingwerf, J. Merijn Schuurmans, Filipe Branco dos Santos, Que Chen

Publikováno v: Metabolic Engineering, 52, 68-76
Metabolic Engineering, 52, pp. 68-76
Metabolic Engineering, 52, 68-76. Academic Press Inc.

To fill the “green absorption gap”, a green absorbing proteorhodopsin was expressed in a PSI-deletion strain (ΔPSI) of Synechocystis sp. PCC6803. Growth-rate measurements, competition experiments and physiological characterization of the proteor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0743146ffc13d0d19257b045febe1c43
https://hdl.handle.net/1887/3202968