Raman spectroscopy of a near infrared absorbing proteorhodopsin: Similarities to the bacteriorhodopsin O photointermediate.

Autor: Gaoxiang Mei, Natalia Mamaeva, Srividya Ganapathy, Peng Wang, Willem J DeGrip, Kenneth J Rothschild

Publikováno v: PLoS ONE, Vol 13, Iss 12, p e0209506 (2018)

Microbial rhodopsins have become an important tool in the field of optogenetics. However, effective in vivo optogenetics is in many cases severely limited due to the strong absorption and scattering of visible light by biological tissues. Recently, a

Externí odkaz: https://doaj.org/article/b0ec26e9fe2a4f94be076c0cd6347cc0

Optical switching between long-lived states of opsin transmembrane voltage sensors

Autor: Gaoxiang Mei, Cesar M Cavini, Willem J. DeGrip, Natalia Mamaeva, Peng Wang, Kenneth J. Rothschild

Publikováno v: Photochemistry and Photobiology, 97(5): 100, pp. 1001-1015.
Photochemistry and Photobiology
Photochemistry and Photobiology, 97(5): 100, 1001-1015.
Photochemistry and Photobiology, 97(5), 1001-1015

Opsin‐based transmembrane voltage sensors (OTVSs) are membrane proteins increasingly used in optogenetic applications to measure voltage changes across cellular membranes. In order to better understand the photophysical properties of OTVSs, we used

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f01f30e7c53e938956a0feb2049840a6
https://repository.ubn.ru.nl/handle/2066/244843

Pre-resonance stimulated Raman scattering spectroscopy and imaging of membrane potential using near-infrared rhodopsins

Autor: Hyeon Jeong Lee, Natalia Mamaeva, Kenneth J. Rothschild, Kai-Chih Huang, Willem J. DeGrip, Ji-Xin Cheng, Gaoxiang Mei

Publikováno v: Multiphoton Microscopy in the Biomedical Sciences XIX.

Voltage imaging has become an emerging technique to record membrane potential change in living cells. Yet, compared to the conventional electrophysiology, imaging approaches are still limited to relative membrane potential changes, losing important i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8bbce3432e7cd4865064cab0e4e73274
https://doi.org/10.1117/12.2506833

Heterologous expression of melanopsin: Present, problems and prospects

Autor: Willem J. DeGrip, Nazhat Shirzad-Wasei

Publikováno v: Progress in Retinal and Eye Research, 52, pp. 1-21
Progress in Retinal and Eye Research, 52, 1-21

Contains fulltext : 172669.pdf (Publisher’s version ) (Closed access) Melanopsin, the photosensory pigment of specialized mammalian retinal ganglion cells, is involved in various non-image forming tasks such as pupillary light reflex, circadian ent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e70b4e704628776a3b9b504270d48e3b
https://doi.org/10.1016/j.preteyeres.2016.02.001

Insight into the chromophore of rhodopsin and its Meta-II photointermediate by 19F solid-state NMR and chemical shift tensor calculations

Autor: Ulrich Sternberg, Isabelle Fernández Fernández, Johan Lugtenburg, Andreas Brinkmann, Arno P. M. Kentgens, Petra H. M. Bovee-Geurts, Willem J. DeGrip

Publikováno v: Physical Chemistry Chemical Physics, 20(48), 30174-30188
PCCP Physical Chemistry Chemical Physics, 20, 48, pp. 30174-30188
PCCP Physical Chemistry Chemical Physics, 20, 30174-30188

19F nuclei are useful labels in solid-state NMR studies, since their chemical shift and tensor elements are very sensitive to the electrostatic and space-filling properties of their local environment. In this study we have exploited a fluorine substi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e845557e9aae587e74f0fc65f37c7395
https://hdl.handle.net/1887/3203112