Zobrazeno 1 - 10
of 169
pro vyhledávání: '"Willem F. Wolkers"'
Autor:
Sercan Içli, Meisam Soleimani, Harriëtte Oldenhof, Harald Sieme, Peter Wriggers, Willem F. Wolkers
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
Abstract Cryopreservation can be used to store equine oocytes for extended periods so that they can be used in artificial reproduction technologies at a desired time point. It requires use of cryoprotective agents (CPAs) to protect the oocytes agains
Externí odkaz:
https://doaj.org/article/42ec7603b6d742699dfadd8d7f2b2fb8
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
Abstract Even though dried sperm is not viable, it can be used for fertilization as long as its chromatin remains intact. In this study, we investigated drying- and temperature-induced conformational changes of nucleic acids and stallion sperm chroma
Externí odkaz:
https://doaj.org/article/3799dd8fb9e44ecf930c4cf73237f882
Autor:
Dejia Liu, Sükrü Caliskan, Bita Rashidfarokhi, Harriëtte Oldenhof, Klaus Jung, Harald Sieme, Andres Hilfiker, Willem F. Wolkers
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
Abstract Freeze-drying can be used to ensure off-the-shelf availability of decellularized heart valves for cardiovascular surgery. In this study, decellularized porcine aortic heart valves were analyzed by nitroblue tetrazolium (NBT) staining and Fou
Externí odkaz:
https://doaj.org/article/c8342ed5b18f41329cfb21e52d1a6688
Publikováno v:
Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
Abstract Saliva has been widely recognized as a non-invasive, painless and easy-to-collect bodily fluid, which contains biomarkers that can be used for diagnosis of both oral and systemic diseases. Under ambient conditions, salivary biomarkers are su
Externí odkaz:
https://doaj.org/article/24d31be547c8401abb3f6c4fde07df31
Autor:
Miao Zhang, Harriëtte Oldenhof, Bulat Sydykov, Judith Bigalk, Harald Sieme, Willem F. Wolkers
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
Abstract The aim of this study was to investigate preservation of biomolecular structures, particularly DNA, in freeze-dried fibroblasts, after loading with trehalose via freezing-induced uptake. Cells were freeze-dried with trehalose alone or in a m
Externí odkaz:
https://doaj.org/article/6818fc6151594e72a6ebc0ad9c7eefd8
Autor:
Sabra Zouhair, Eleonora Dal Sasso, Sugat R. Tuladhar, Catia Fidalgo, Luca Vedovelli, Andrea Filippi, Giulia Borile, Andrea Bagno, Massimo Marchesan, De Rossi Giorgio, Dario Gregori, Willem F. Wolkers, Filippo Romanato, Sotirios Korossis, Gino Gerosa, Laura Iop
Publikováno v:
Biomolecules, Vol 10, Iss 3, p 371 (2020)
Xenogeneic pericardium-based substitutes are employed for several surgical indications after chemical shielding, limiting their biocompatibility and therapeutic durability. Adverse responses to these replacements might be prevented by tissue decellul
Externí odkaz:
https://doaj.org/article/e9b4a31f55b74f53afdc38a950f1d784
Autor:
Harriëtte Oldenhof, Huaqing Yang, Xing Luo, Willem F. Wolkers, David Pruß, Harald Sieme, Dejia Liu, Jan Hegermann
Publikováno v:
Cryobiology. 101:67-77
Stallion sperm is typically cryopreserved using low cooling rates and low concentrations of cryoprotective agents (CPAs). The inevitable water-to-ice phase transition during cryopreservation is damaging and can be prevented using vitrification. Vitri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20992f5b1364ec8ad3296beb4309b927
https://doi.org/10.1016/j.cryobiol.2021.05.007
https://doi.org/10.1016/j.cryobiol.2021.05.007
Publikováno v:
Biochimica et biophysica acta. General subjects. 1867(1)
In this study we investigated hydrogen bonding interactions in hydrated and frozen solutions of different cryoprotective agents (CPAs) including dimethyl sulfoxide, glycerol, ethylene glycol, propylene glycol, and trehalose. We also investigated the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ef6da928f49caf7b72c4bd480bc7b3c
https://pubmed.ncbi.nlm.nih.gov/36243203
https://pubmed.ncbi.nlm.nih.gov/36243203
Autor:
Igor I. Katkov, Oleksandr Gryshkov, Vitalii Mutsenko, Barbara Dovgan, Ariana Barlič, Janja Dermol-Černe, Damijan Miklavčič, Bulat Sydykov, Willem F. Wolkers, Tamara Pezić, Birgit Glasmacher
Publikováno v:
Cryobiology. 91:104-114
Cryopreservation is the universal technology used to enable long-term storage and continuous availability of cell stocks and tissues for regenerative medicine demands. The main components of standard freezing media are dimethyl sulfoxide (hereinafter
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::aaed30d396aacb1e4f577fa1511e7bde
https://doi.org/10.1016/j.cryobiol.2019.10.002
https://doi.org/10.1016/j.cryobiol.2019.10.002
Membrane permeability of Jurkat cells to cryoprotective agents determined by flow imaging microscopy
Autor:
Alexandra Roesch, Roland Windisch, Christian Wichmann, Willem F. Wolkers, Gideon Kersten, Tim Menzen
Publikováno v:
Cryobiology. 109:51
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::00f6b236d212980d040ccc4f633fcda0
https://doi.org/10.1016/j.cryobiol.2022.11.164
https://doi.org/10.1016/j.cryobiol.2022.11.164