Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Will Skyrud"'
Autor:
Matthew Jenner, Yang Hai, Hong H. Nguyen, Munro Passmore, Will Skyrud, Junyong Kim, Neil K. Garg, Wenjun Zhang, Rachel R. Ogorzalek Loo, Yi Tang
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-12 (2023)
Abstract Siderophores belonging to the ferrichrome family are essential for the viability of fungal species and play a key role for virulence of numerous pathogenic fungi. Despite their biological significance, our understanding of how these iron-che
Externí odkaz:
https://doaj.org/article/55fce6b1e0a0485a86f2208747b053b0
Autor:
Mitchell G. Thompson, Jacquelyn M. Blake-Hedges, Jose Henrique Pereira, John A. Hangasky, Michael S. Belcher, William M. Moore, Jesus F. Barajas, Pablo Cruz-Morales, Lorenzo J. Washington, Robert W. Haushalter, Christopher B. Eiben, Yuzhong Liu, Will Skyrud, Veronica T. Benites, Tyler P. Barnum, Edward E. K. Baidoo, Henrik V. Scheller, Michael A. Marletta, Patrick M. Shih, Paul D. Adams, Jay D. Keasling
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Hydroxyglutarate synthase (HglS) converts 2-oxoadipate to D-2- hydroxyglutarate during lysine catabolism in bacteria. Here the authors use structural and biochemical approaches to show that HglS acts via successive decarboxylation and intramolecular
Externí odkaz:
https://doaj.org/article/a6c6f19696ff49aaac6e98cf1d7e8b64
Autor:
Mitchell G. Thompson, Jacquelyn M. Blake-Hedges, Pablo Cruz-Morales, Jesus F. Barajas, Samuel C. Curran, Christopher B. Eiben, Nicholas C. Harris, Veronica T. Benites, Jennifer W. Gin, William A. Sharpless, Frederick F. Twigg, Will Skyrud, Rohith N. Krishna, Jose Henrique Pereira, Edward E. K. Baidoo, Christopher J. Petzold, Paul D. Adams, Adam P. Arkin, Adam M. Deutschbauer, Jay D. Keasling
Publikováno v:
mBio, Vol 10, Iss 3 (2019)
ABSTRACT Despite intensive study for 50 years, the biochemical and genetic links between lysine metabolism and central metabolism in Pseudomonas putida remain unresolved. To establish these biochemical links, we leveraged random barcode transposon se
Externí odkaz:
https://doaj.org/article/a71a6236dda04344a17ff1e8947b3078
Publikováno v:
ACS Catalysis. 10:3360-3364
The conversion of shikimate to cyclohexanecarboxyl-CoA (CHC-CoA) involves an exquisite orchestra of multiple enzymes to proceed stereospecifically and avoid aromaticity, but little is known regardi...
Autor:
Wenjun Zhang, Ella Zafrir, Colin C. Barber, Jeffrey S Li, Nicolaus A. Herman, Xuejun Zhu, Yongle Du, Wenlong Cai, Will Skyrud
Publikováno v:
Journal of industrial microbiology & biotechnology, vol 47, iss 3
J Ind Microbiol Biotechnol
J Ind Microbiol Biotechnol
Clostridium saccharoperbutylacetonicum N1-4 (Csa) is a historically significant anaerobic bacterium which can perform saccharolytic fermentations to produce acetone, butanol, and ethanol (ABE). Recent genomic analyses have highlighted this organism
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d520a2e970ed6c7145f736a208bc61e
https://escholarship.org/uc/item/6nb1k8kf
https://escholarship.org/uc/item/6nb1k8kf
Autor:
Henrik Vibe Scheller, William M. Moore, Jesus F. Barajas, Paul D. Adams, Michael S. Belcher, Jacquelyn M. Blake-Hedges, Will Skyrud, Robert W. Haushalter, Patrick M. Shih, Yuzhong Liu, Pablo Cruz-Morales, Veronica T. Benites, John A. Hangasky, Christopher B. Eiben, Michael A. Marletta, Edward E. K. Baidoo, Jose Henrique Pereira, Jay D. Keasling, Lorenzo J. Washington, Mitchell G. Thompson, Tyler P. Barnum
Publikováno v:
Thompson, M G, Blake-Hedges, J M, Pereira, J H, Hangasky, J A, Belcher, M S, Moore, W M, Barajas, J F, Cruz-Morales, P, Washington, L J, Haushalter, R W, Eiben, C B, Liu, Y, Skyrud, W, Benites, V T, Barnum, T P, Baidoo, E E K, Scheller, H V, Marletta, M A, Shih, P M, Adams, P D & Keasling, J D 2020, ' An iron (II) dependent oxygenase performs the last missing step of plant lysine catabolism ', Nature Communications, vol. 11, no. 1, 2931 . https://doi.org/10.1038/s41467-020-16815-3
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Nature Communications
Nature communications, vol 11, iss 1
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
Nature Communications
Nature communications, vol 11, iss 1
Despite intensive study, plant lysine catabolism beyond the 2-oxoadipate (2OA) intermediate remains unvalidated. Recently we described a missing step in the D-lysine catabolism of Pseudomonas putida in which 2OA is converted to D-2-hydroxyglutarate (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19d25b9f0ff3e8f1b5538f7cd43c4598
https://orbit.dtu.dk/en/publications/bebee354-ccb6-4b22-b431-1433abb4be01
https://orbit.dtu.dk/en/publications/bebee354-ccb6-4b22-b431-1433abb4be01
Autor:
Mitchell G, Thompson, Zak, Costello, Niklas F C, Hummel, Pablo, Cruz-Morales, Jacquelyn M, Blake-Hedges, Rohith N, Krishna, Will, Skyrud, Allison N, Pearson, Matthew R, Incha, Patrick M, Shih, Hector, Garcia-Martin, Jay D, Keasling
Publikováno v:
ACS synthetic biology. 8(10)
A significant bottleneck in synthetic biology involves screening large genetically encoded libraries for desirable phenotypes such as chemical production. However, transcription factor-based biosensors can be leveraged to screen thousands of genetic
Autor:
Nicholas C. Harris, Frederick F. Twigg, Paul D. Adams, Christopher J. Petzold, Will Skyrud, Adam P. Arkin, Jacquelyn M. Blake-Hedges, Jesus F. Barajas, Samuel C. Curran, Mitchell G. Thompson, Pablo Cruz-Morales, Rohith N. Krishna, William A. Sharpless, Veronica T. Benites, Jennifer W. Gin, Edward E. K. Baidoo, Jose Henrique Pereira, Jay D. Keasling, Adam M. Deutschbauer, Christopher B. Eiben
Publikováno v:
mBio, Vol 10, Iss 3, p e02577-18 (2019)
Thompson, M G, Blake-Hedges, J M, Cruz-Morales, P, Barajas, J F, Curran, S C, Eiben, C B, Harris, N C, Benites, V T, Gin, J W, Sharpless, W A, Twigg, F F, Skyrud, W, Krishna, R N, Pereira, J H, Baidoo, E E K, Petzold, C J, Adams, P D, Arkin, A P, Deutschbauer, A M & Keasling, J D 2019, ' Massively parallel fitness profiling reveals multiple novel enzymes in Pseudomonas putida lysine metabolism ', mBio, vol. 10, no. 3, e02577-18 . https://doi.org/10.1128/mBio.02577-18
mBio, vol 10, iss 3
mBio, Vol 10, Iss 3 (2019)
Thompson, M G, Blake-Hedges, J M, Cruz-Morales, P, Barajas, J F, Curran, S C, Eiben, C B, Harris, N C, Benites, V T, Gin, J W, Sharpless, W A, Twigg, F F, Skyrud, W, Krishna, R N, Pereira, J H, Baidoo, E E K, Petzold, C J, Adams, P D, Arkin, A P, Deutschbauer, A M & Keasling, J D 2019, ' Massively parallel fitness profiling reveals multiple novel enzymes in Pseudomonas putida lysine metabolism ', mBio, vol. 10, no. 3, e02577-18 . https://doi.org/10.1128/mBio.02577-18
mBio, vol 10, iss 3
mBio, Vol 10, Iss 3 (2019)
Despite intensive study for 50 years, the biochemical and genetic links between lysine metabolism and central metabolism inPseudomonas putidaremain unresolved. To establish these biochemical links, we leveraged Random Barcode Transposon Sequencing (R
Autor:
Allison N. Pearson, Zak Costello, Mitchell G. Thompson, Jacquelyn M. Blake-Hedges, Pablo Cruz-Morales, Niklas F. C. Hummel, Jay D. Keasling, Matthew R. Incha, Will Skyrud, Patrick M. Shih, Hector Garcia-Martin, Rohith N. Krishna
Publikováno v:
Thompson, M G, Costello, Z, Hummel, N F C, Cruz-Morales, P, Blake-Hedges, J M, Krishna, R N, Skyrud, W, Pearson, A N, Incha, M R, Shih, P M, Garcia-Martin, H & Keasling, J D 2019, ' Robust characterization of two distinct glutarate sensing transcription factors of pseudomonas putida L-Lysine metabolism ', ACS Synthetic Biology, vol. 8, no. 10, pp. 2385-2396 . https://doi.org/10.1021/acssynbio.9b00255
A significant bottleneck in synthetic biology involves screening large genetically encoded libraries for desirable phenotypes such as chemical production. However, transcription factor-based biosensors can be leveraged to screen thousands of genetic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8517454acbed17d6faa0f3f87e0bec1
https://orbit.dtu.dk/en/publications/7d8d07a0-af19-49f3-8bfa-f80da7a0a058
https://orbit.dtu.dk/en/publications/7d8d07a0-af19-49f3-8bfa-f80da7a0a058
Antimycins are a family of natural products possessing outstanding biological activities and unique structures, which have intrigued chemists for over a half century. Of particular interest are the ring-expanded antimycins that show promising antican
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d47b5c4c4eb04acf536ff5177d79f026
https://eprints.whiterose.ac.uk/130177/8/acschembio.pdf
https://eprints.whiterose.ac.uk/130177/8/acschembio.pdf