Methods for Precisely Localised Transfer of Cells or DNA into Early Postimplantation Mouse Embryos

Autor: Huang, Yali, Wilkie, Ron, Wilson, Valerie

Publikováno v: Huang, Y, Wilkie, R & Wilson, V 2015, ' Methods for Precisely Localised Transfer of Cells or DNA into Early Postimplantation Mouse Embryos ', Journal of Visualized Experiments (JoVE), no. 106, e53295 . https://doi.org/10.3791/53295

We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the prec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______3094::e67d8b6db8b5d5283f41b052e4880bbb
https://hdl.handle.net/20.500.11820/d8777dad-e42b-4b8e-877d-7003d6da6bbb

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Expression of a novel homeobox gene Ehox in trophoblast stem cells and pharyngeal pouch endoderm.

Autor: Jackson M; John Hughes Bennett Laboratory, University of Edinburgh, Scotland, United Kingdom., Baird JW, Nichols J, Wilkie R, Ansell JD, Graham G, Forrester LM

Publikováno v: Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2003 Dec; Vol. 228 (4), pp. 740-4.